ABSTRACT
Background: Otitis media is defined as an infection of themiddle ear fluid and is the second most common diagnosis inthe emergency department following upper respiratoryinfections. Hence; under the light of above mentioned data, weplanned the present study to assess microbiological profile ofpatients with otitis media.Materials & Methods: A total of 100 patients with otitis mediawere included in the present study. From the diseased ear ofall the patients, ear discharge was obtained use sterilizedswabs. All the swabs were inoculated in 5% sheep blood agar(BA), MacConkey's agar and chocolate agar (CA) andSabouraud Dextrose Agar with chloramphenicol (0.05%),followed by incubation at 28°C and 37°C. The slants were laterexamined for gross and the microscopic morphology of thefungi.Results: Among aerobes, the predominant species identifiedwere Pseudomonas aeruginosa, Kebsiella penmoniae,Escherichia coli and Diptheoides. Among anaerobesClostridium species, Peptococcus speciesand Peptostreptococcus species were the most commonlyidentified species. Among fungal isolates, the most commonlyisolated species were that of Aspergillus niger, Aspergillusfumigatus and Candida species.Conclusion: Aerobic bacteria are the most common isolatesamong otitis media patients.