Estimate the Serum Trace Minerals of Female Black Bengal Goats at Different Stages

Serum hormone level was estimated to find out FSH, LH and total estrogen for all the animals under three treatment groups from 20 to 55 week. The FSH level (from 0.56 to 1.32 ng/ml; 0.91 to 1.91 ng/ml and 0.46 to 1.36 ng/ml for first, second and third treatment group, respectively) was higher in the second group; and third group had lowest level out of the three groups. The serum LH level (0.46 to 1.40 ng/ml and 0.27 to 1.91 ng/dl in first and second treatment group, respectively) showed similar pattern like FSH. The total estrogen was higher in the first treatment group (1.08 to 4.2 pg/ml) than that of the second group (0.14 to 2.30pg/ml) and third group ((0.04 to 2.37 pg/ml)). The serum biochemical profiles of the animals under three groups were estimated by serum glucose, total protein in blood and serum cholesterol levels. The glucose level varied from 65.66 to 77.11, 50.03 to 63.78 and 55.22 to 68.66 mg/dl for the first, second and third group, respectively, with higher value at the time of service. The total protein level did not vary much among the animals under three treatment groups (64.45 to 78.55 g/l). The serum cholesterol level varied between 50.17 to 110.05 mg/dl among all the animals irrespective of groups. During prepubertal period this value was low, but it was higher during pubertal period, time of several other services and towards pregnancy for all groups of animals. Regarding the serum trace mineral status, serum copper level showed higher value from 7 months onwards; serum zinc and iron level were almost similar in first and second group; and the third group showed lower value for all the trace minerals. All the good effects noticed in the first treatment group were due to the non-hormonal factors applied to them through nutrition and buck. The effects in the second group of animals was due to the nutritional effect, whereas, the third group showed poor performance without any support from nutrition and social interaction through buck.

A study to assess anxiety, stress and depression among private medical practitioners during COVID-19 pandemic: An observational study

Objectives: This study aims to assess the magnitude of anxiety, stress, and depression among private practitioners during the COVID pandemic 2020. Materials and Methods: A descriptive, observational and cross-sectional study was done using a semi-structured questionnaire through electronic media in the form of Google Forms. Patient health questionnaire 4 (PHQ4) and Perceived Stress Scale-10 were incorporated into the questionnaire to assess the psychological status in November 2020. Results: A total of 157 private practitioners participated in the study. Males constituted 60%. Participants have mean age (SD) of 44.2 (6.9) years with work experience and a mean (SD) of 16.3 (6.78) years. The anxiety and depression subscales of the PHQ-4 scale showed that 54.7% of the private practitioners have anxiety subscale ?3 and 28% have depression subscale >3 needing further psychiatric evaluation. Significant predictors of anxiety and depression were female gender, younger and less experienced, and those providing inpatient services in COVID care isolation facilities. Conclusion: There is a high prevalence of anxiety, stress, and depression among private practitioners comparable with other countries.

Omicron and vaccine booster dose – an update

Mutations in coronavirus is not unheard off. The variant, Omicron B.1.1.529 has overall 50 mutations and is considered as a variant of concern. Though the new variant has shown changes throughout its genome, S gene changes predominate. Significant changes in the S encoding gene, S gene, influences not only the transmissibility properties of SARS-CoV-2 but also the efficacy of existing vaccines. Increased transmissibility and ability of this variant to evade host immune responses conferred either due to past infections or due to vaccination driving increase in number of infections. However, the severity of the infections is low in comparison to its predecessors. A meta-analysis of ten studies analyzing the effect of COVID-19 vaccine booster dose, it was found studies report 10-to-42-fold increase in omicron neutralization. COVID appropriate behaviors, if followed rigidly can bring a meaningful change in the viral spread and resultant health impacts.

Tillage and nutrient management influence net global warming potential and greenhouse gas intensity in soybean-wheat cropping system

Conservation tillage has proven advantageous in improving soil health and productivity. However, the greenhouse gases (GHGs) emission and intensity from different conservation tillage and nutrient management systems under Indian conditions are less understood. Therefore, here, we compared the effect of tillage and nutrient management on GHGs emissions, net global warming potential (NGWP), and greenhouse gas intensity (GHGI) from a field experiment under five years in a soybean-wheat cropping system in the Vertisols. The tillage treatments comprised of reduced tillage (RT) and no tillage (NT). The three nutrient management treatments included application of 100% NPK (T1), 100% NPK + 1.0 Mg FYM-C ha-1 (T2), 100% NPK +2.0 Mg FYM-C ha-1 (T3). The results showed significantly higher SOC sequestration under NT (1388 kg ha-1 yr-1) followed byRT (1134 kg ha-1 yr-1) with application of FYM (2.0 Mg C ha-1) (T3) every year. Across tillage, integrated nutrient management(T2 and T3) lowered NGWP and GHGI compared to NPK (T1). The GHGI of NT system was less by 33% compared to RT. The results suggest that GHGs mitigation and sustained food production in the soybean-wheat system can be achieved in NT and RT with integrated use of organic and inorganic fertilizer as the major component of nutrient management.

A clinico-epidemiological study of the first outbreak of Nipah virus in India – report from ground zero

Introduction: The first Nipah Virus (NiV) outbreak occurred in India in the year 2001 at Siliguri. The second outbreak happened at Nadia in 2007. Nipah Virus exhibits neurological and pneumonic tropism with the predominant clinical presentation being encephalitis in humans. Material and Methods: The present study was a record based prospective study on 67 cases admitted with pyrexia of unknown origin in North Bengal Medical College during the period from 18.02.2001 to 30.02.2001 and a parallel study on epidemiological record carried out by PSM department also taken into account. All necessary investigations including autopsy examination, pathological, and microbiological study were done. Results: There was a clustering of cases around Bhaktinagar. There was a strong H/O Medinova Nursing Home Contact among the patients. 18 out of 20 cases were staff of that Nursing Home. Serum samples tested show NiV specific IgM and IgG in 9 out of 17 samples with one sample which was positive for IgG only suggesting past infection. The cases were admitted with predominant neurological symptoms (53.73% cases) but about 80% recovered with no residual neuro deficit. The natural reservoir of NiV is present in Bangladesh and in Northern India. Conclusion: When NiV infection is suspected, infection control practices must be strengthened to avoid an outbreak in a hospital setting. Here the present study is presenting the experience in the first outbreak of the Nipah virus in India at Siliguri for awareness of clinical personnel to control further outbreak at the very beginning.

Effectiveness of first-aid training on school children of urban area of Sambalpur District, Odisha

Background: Delay in accessing appropriate medical care and/or lack of knowledge regarding treatment results in the death of the injured which can be avoided by immediate resuscitation measures. First aid, as the name implies, is the first care given to a victim of an accident, injury, or sudden illness, before the provision of advanced medical care. First aid should be aimed to preserve life, promote recovery, and prevent worsening of the victim’s condition. Objectives: The present study was aimed to assess the knowledge of school students regarding first-aid management and to evaluate the effectiveness of education on selected first-aid measures among school students. Materials and Methods: A total of 95 students of 6th, 7th, and 8th standard students were given a self‑administered questionnaire for assessing their baseline knowledge about management of common injuries followed by educational intervention. Post-intervention evaluation of their knowledge acquisition was done after 10 days with the same questionnaire. Results: The objective of providing knowledge was accomplished with maximum students retaining knowledge shown as per the percentage increase in post-test scores. The maximum increase was seen in epistaxis (65.26%), head injury (44.21%), choking (42.11%), snakebite (52.94%), and dog bite (42.11%) which signifies less number of students had knowledge about these first-aid measures. Paired t-test showed a statistically significant difference in the pre- and post-education scores. Conclusion: Inculcating first‑aid training in the school curriculum can be a fruitful investment in ensuring proper and timely management of illnesses and injuries.

Morphological Variations of the Thyroid Gland in the North Indians: A Cadaveric Study with its Clinical Relevance

Introduction: The morphological variations of the thyroid gland are not an uncommon phenomenon and may due to embryological remnant or non-specific development of different parts of it. Prior anatomical knowledge of these variations is of immense importance to prevent catastrophies during or after thyroid surgeries. Therefore, in this study we aimed to investigate the prevalence of morphological variations of the thyroid glands in north Indian cadavers. Subjects and Methods: This study was conducted on 50 formalin embalmed adult human cadavers aging between 40–65 years, of which 40 were males and 10 were females. Thyroid glands were dissected and examined properly for the presence of pyramidal lobe, levator glandulae thyroidae, accessory thyroid tissue and complete absence of isthmus. Results: The pyramidal lobe was present in 9 (18%) and frequently arising from the right side of the isthmus. LGT was found in 7 (14%) and almost in all cases it was extending from the apex of the pyramidal lobe to the hyoid bone. Only 2 (4%) cadavers did not show an isthmus while accessory thyroid tissue was found only in one case. Morphological variations were more common in females than in males and the difference was statistically significant (p value<0.05). The means of all measured parameters were higher in female than in male but these gender differences were not significant (p>0.05). Conclusion: Morphological variation of the thyroid gland is a common phenomenon, particularly in female. Hence it requires proper detection and documentation prior to any thyroid surgery, so that iatrogenic catastrophies can be avoided

Food and feeding behaviour of Asiatic elephant (Elephas maximus Linn.) in Kuldiha Wild Life Sanctuary, Odisha, India.

The feeding behaviour of Asiatic elephant (Elephas maximus) with food reference was studied in Kuldiha Wildlife Sanctuary in Odisha during 2007 to 2009. Though the study area houses a good number of plant species only 71 species were identified as elephant fodder plants. The food trail of elephant was observed as twig breaking, bark peeling, branch breaking, stem twisting uprooting and flower plucking in different regions of study area during different seasons. Alteration of predominantly browsing strategy with that of grazing around the year was related to seasonal variation of food plants. Consumption of tree species (56%) was highest as compared to shrubs (20%), herbs (14%) and climbers (10%). A high degree of variation in dicot- monocot ratio (61:10)) was marked during identification of elephant fodder plant by direct observation. Microscopic analysis of dung showing a high degree of variation in average dicot- monocot ratio suggested that the food plant selection of elephant was highly opportunistic and seasonal. The elephants extensively fed on the plant species like Careya arborea, Kydia calycina, Helicteres isora, Mallotus philippinensis, Aegle marmelos, Zizyphus mauritiana, Bauhinia racemosa, Bauhinia vahlii, Mimosa pudica, Asparagus racemosus, Smilax zeylanica and Diosporea species. They were fond of Madhuca indica (Mahula) flowers in winter and fruits of Mangifera indica (Mango) in summer. They were never found feeding on Tectona grandis and Eucalyptus maculate inside the study area.

Assessment of the current practice regarding Diarrhea Management by pharmacists/chemist in Moradabad.

Diarrhea is one of the GIT disorders that create discomfort of normal life. Diarrhea is defined by the World Health Organization as having 3 or more loose or liquid stools per day or as having more stools than is normal for that person. It may be due to infection by viruses, Bacteria and parasites etc. Generally in small cities large number of diarrheal patients visit chemist shop and took medicine with or without advice/precaution from over the counter. Present study deal with specific aim focus on evaluating the role of Pharmacist/Chemist in management of diarrhea. Pharmacist helped in out in managing diarrhea to some extent by prescribing drugs either in single or combinations form but they don’t know about the which patients to test, what tests to order, what accurate medical treatments to use, and what steps to take in case of acute as well as in chronic case of diarrhea.

Lunar rhythm in the planktonic biomass of the Nicco Park lake, Bhubaneswar.

The lunar cycle imparts certain stimulatory effects on the rhythmic behaviour of plankton and their life processes by which they actively migrate to the surface and gradually sink as the moon fades. This may also result in morphological changes in organisms, such as Lorica which becomes smaller towards the full moon night by changing the size of postero-lateral spines and surface area. In addition, cyclomorphic forms have been observed in zooplankton, which appears to be a suitable achivement for swarming and for the differentiation of sex by secondary sexual characteristics. Lunar rhythm in plankton appears to be governed by an internal clock, that is the "Biological clock".

Purification and partial characterization of PfHRP-II protein of Plasmodium falciparum.

The human malarial parasite Plasmodium falciparum secretes various intra-and extra-cellular proteins during its asexual life cycle in human RBC. Histidine rich protein-II (HRP-II) is one of the most prominent proteins, found to be secreted by P. falciparum throughout the asexual cycle with the peak during mature schizont stage of the parasite development in human IRBC. The high histidine content (35% of the total amino acids in protein) of this protein suggested the potential to bind divalent metal ions. We have demonstrated by metal chelate chromatography, an extraordinary capacity of HRP-II to bind nickel ions (Ni++) and employed this characteristic to purify the extra-cellular HRP-II protein secreted by P. falciparum from culture supernatant. The identity of the purified protein was verified by the relative molecular weight on SDS-PAGE, by reacting with polyclonal antibodies directed against it using Western blot technique.

Diltiazem vs. nifedipine in chilblains: a clinical trial.

BACKGROUND: The treatment of chilblains remains unsatisfactory. Nifedipine in higher doses remains the mainstay of treatment. AIMS: To compare the efficacy of diltiazem with that of nifedipine, and to determine the efficacy of nifedipine in lower doses, 36 chilblains cases were studied. MATERIAL AND METHODS: Group A (12 patients) was treated with diltiazem 60 mg thrice daily, and Group B (24 patients) with 10 mg nifedipine thrice daily till complete relief and then maintained on a sustained release preparation of nifedipine 20 mg twice daily. RESULTS: Two patients in Group A showed complete relief in 7 days, and 3 patients in about 21 days, but in 7 cases there was little or no response. In group B, 21 cases showed 80-90% relief by the fourteenth day. CONCLUSIONS: We conclude that nifedipine remains the drug of choice in chilblains but can be used in a smaller dosage in the Indian population. Diltiazem is less effective in the conventional dose, which may be optimized.

Epidermolysis bullosa.

A case of recurrent vesiculobullous eruptions over shins, clinically diagnosed as epidermolysis bullosa of pretibial variety confirmed by electron microscope is reported here as a rare variety of localised epidermolysis bullosa (EB).

Fine needle aspiration as a primary diagnostic procedure of breast lumps.

Two hundred and sixty-seven patients from the ages between 17 to 70 years with breast lumps attending the Cytology Clinic of the Out-patients Department and Cytology Section of the Department of Pathology, M.K.C.G. Medical College, Berhampur (Orissa), were subjected to Fine-Needle Aspiration Cytology (FNAC). Sixty-four patients were discarded from study because of non-availability of biopsy specimens and unsuitable smears. When cytological results were compared with those of histological sections, 100 per cent correct diagnosis was made in chronic mastitis and lobular carcinoma, 94.24 per cent in infiltrating duct carcinoma and 84.60 percent in duct papilloma and with the maximum over diagnosis to the tune of 14.75 per cent was found in fibro-adenoma. An overall correct diagnosis was made in 88.67 percent of cases by FNAC.

Bilateral involvement of sole and palm in leprosy. A case report.

A case of BT leprosy in reaction with lesions over uncommon (immune) sites like palm and sole has been reported.

Multiple synovial swellings in BT leprosy. A case report.

A case of Leprosy with multiple synovial swellings has been reported. Rarely these swellings may be an initial presentation and at sites other than dorsum of hand or ankle as was in this case. The literature on the subject is briefly reviewed.