ABSTRACT
We sought to evaluate the hypolipidemic activity of extracts of Bidens odorata and Brickellia eupatorioides using a model of hyperlipidemia induced in rats by Triton WR-1339 [300mg/kg intraperitoneally]. The rats were divided into 5 groups of 3 rats each: normal control group, hyperlipidemic control group, hyperlipidemic with 20_mg/kg atorvastatin, hyperlipidemic with 300 mg/kg B. odorata extract, and hyperlipidemic with 300mg/kg B. eupatorioides extract, respectively. After 10 d of treatment by intragastric administration, the extract of B. odorata caused a significant decrease of serum total cholesterol and triglyceride levels without altering the liver enzymes aspartate transaminase and alanine aminotransferase. In addition, the extract had antioxidant potential as shown by the 2,2?-diphenyl-1- picrylhydrazyl technique. These findings indicate that B. odorata has potential as a hypolipidemic agent and might be beneficial in treatment of hyperlipidemia and atherosclerosis
ABSTRACT
Hamelia patens is widely used in the traditional medicine of Mexico and Central America for the treatment of illnesses associated with inflammatory processes. In this study, antioxidant and hepatoprotective activity were assayed on the methanolic crude [ME], hexane [HE], ethyl acetate [AE], and butanol [BE] extracts of H. patens. The total phenolic content [TPC] as mg of gallic acid equivalents per g of dry extract was determined by Folin-Ciocalteu's method [ME=141.58 +/- 11.99, HE=33.96 +/- 1.13, AE=375.18 +/- 13.09, BE=132.08 +/- 3.62], and antioxidant activity by 2,2-diphenyl-1- picryl-hydrazyl [DPPH] free radical-scavenging method [EC50 ME=77.87 +/- 5.67, HE=236.64 +/- 26.32, AE=45.87 +/- 2.24, BE=50.97 +/- 0.85microg/mL]. Hepatoprotective activity was evaluated through AST activity on HepG2 cells subjected to damage with CCl4 [ME=62.5 +/- 3.41, HE=72.25 +/- 2.87, AE=63.50 +/- 4.20, BE=43.74 +/- 4.03]. BE showed the greater hepatoprotective activity and a good antioxidant capacity, while HE did not show hepatoprotective or antioxidant activity. Cytotoxicity was evaluated on Vero cells cultures; none showed significant toxicity