ABSTRACT
RESUMEN: El parvovirus B19 (B19V), descubierto en 1974, es un patógeno humano de distribución global. Si bien su infección puede transcurrir de manera asintomática, es conocido por ser capaz de causar un amplio espectro de manifestaciones clínicas como son el eritema infeccioso, artropatías, crisis aplásica en individuos con recambio acelerado de glóbulos rojos por una enfermedad de base, y anemia crónica en inmunocomprometidos, entre otras patologías en las que aún se investiga la causalidad del B19V. La infección durante el embarazo es un riesgo potencial para el feto...
ABSTRACT: Parvovirus B19 (B19V), discovered in 1974, is a pathogen of global distribution. While the infection can be asymptomatic, it is known for being able to cause a wide range of clinical manifestations such as erythema infectiosum (or fitth disease), arthropathy, aplastic crisis in persons with accelerated red blood cell renewal due to a base disease, and chronic anemia in immunocompromised individuals, among other pathologies inwhich B19V causality is still under research. In addition, infection during pregnancy is a potential risk for the fetus...
Subject(s)
Male , Female , Humans , Biomarkers, Pharmacological/analysis , Parvoviridae Infections/diagnosis , Parvoviridae Infections/immunology , /immunology , Argentina/epidemiologyABSTRACT
Human bocavirus (HBoV) is a parvovirus whose association with respiratory disease is currently under investigation. OBJECTIVE: To determine HBoV prevalence in children with lower acute respiratory infection. METHODS: We investigated HBoV in 433 nasopharyngeal aspirates collected in 2007-2009 from children 0 to 5 years old hospitalized with bronchiolitis or pneumonia in Córdoba, Argentina. RESULTS: The general prevalence of HBoV was 21.5 percent and the positive cases (HBoV+) were more frequent during winter and spring. The mean age of HBoV+ patients was 6.9 months, with 87.1 percent of the detections corresponding to infants less than 1 year old (among which the prevalence of HBoV was 26.3 percent in patients < 3 months of age, 22.1 percent in 3 to 6 months, 25.3 percent in 6 to 9 months, and 18.8 percent in 9 to 12 months). The sequence analysis of the NP1 coding region of 15 isolates showed that all isolates from Cordoba were HBoV1 which exhibited a homology of nearly 100 percent both among themselves and with the originally discovered virus from 2005. CONCLUSION: Overall, our results indicate that HBoV is a significant pathogen that contributes to acute respiratory infection both on its own and during coinfection with other viruses.
Subject(s)
Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Bronchiolitis, Viral/virology , Human bocavirus , Parvoviridae Infections/virology , Pneumonia, Viral/virology , Acute Disease , Argentina/epidemiology , Bronchiolitis, Viral/epidemiology , DNA, Viral/analysis , Human bocavirus/genetics , Human bocavirus/isolation & purification , Nasopharynx/virology , Phylogeny , Polymerase Chain Reaction , Prevalence , Parvoviridae Infections/epidemiology , Pneumonia, Viral/epidemiologyABSTRACT
Durante el período 1999-2000, se realizó el aislamiento de Chlamydia trachomatis (C trachomatis) a partir de 132 muestras genitales de una población seleccionada de la ciudad de Córdoba. Las inclusiones chlamydiales se revelaron por coloración de Lugol y por inmunofluorescencia indirecta con anticuerpos monoclonales (IFI-AcMn). Se obtuvieron 43 aislamientos positivos por IFI-AcMn, en cambio, sólo 40 fueron positivos por coloración de Lugol. Los 3 aislamientos IFI positivos y Lugol negativos desarrollaron inclusiones semejantes a las inclusiones chlamydiales, pero en su interior no habían retenido el colorante con iodo. Si como los resultados demuestran, existen cepas de C. trachomatis con nuevas características, el aislamiento en cultivo celular y el revelado por IFI-AcMn sería el método de elección para el diagnóstico de la infección por C. trachomatis