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Zhonghua Bing Li Xue Za Zhi ; (12): 238-241, 2004.
Article in Chinese | WPRIM | ID: wpr-283539

ABSTRACT

<p><b>OBJECTIVE</b>To investigate BCL-6 gene mutations in B-cell non-Hodgkin lymphomas (B-NHL) and their implications in lymphoma pathogenesis.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) and direct DNA sequencing methods were used to identify mutations in the 5'-noncoding region of BCL-6 gene in 135 cases of B-NHL, 5 cases of T-NHL, 5 cases of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and 10 cases of reactive hyperplasia of lymph node.</p><p><b>RESULTS</b>Mutations were identified in 6 cases of nodal DLBCL (27.3%), 4 cases of FL (22.2%), 4 cases of MALT lymphoma (22.2%), 4 cases of extranodal DLBCL (20.7%) and 2 cases of LRH (20%). No mutations were detected in T-NHL and NLPHL (P < 0.05). There were no significant differences in incidences of BCL-6 gene mutations between nodal and extranodal DLBCL (P > 0.05). All mutations were base substitutions and the frequency of single-base change was 0.14 x 10(-2)/bp approximately 0.68 x 10(-2)/bp.</p><p><b>CONCLUSIONS</b>Mutations of the 5'non-coding region of BCL-6 gene may be involved in the pathogenesis and progression of B-NHL. Molecular demonstration of such mutations may provide a marker of lymphomas derived from the germinal center-related B cells.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , 5' Untranslated Regions , Genetics , Base Sequence , DNA-Binding Proteins , Genetics , Lymphoma, B-Cell , Genetics , Pathology , Lymphoma, Non-Hodgkin , Genetics , Pathology , Molecular Sequence Data , Point Mutation , Proto-Oncogene Proteins , Genetics , Proto-Oncogene Proteins c-bcl-6 , Transcription Factors , Genetics
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