ABSTRACT
Objective@#To investigate the potential effect of proteoglycans (PG) and glycosaminoglycans (GAG) on the stability of resin-dentin bonds against artificial saliva storage.@*Methods@#Seventy-two extracted molars were used to obtain standard dentin bonding surface, and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random number. Then the three groups undergone different incubating procedures as follow: specimens in chondroitinase ABC (C-ABC) group were incubated with C-ABC, specimens in trypsin (TRY) group were incubated with trypsin, and specimens in the control group were incubated with deionized water. All specimens were incubated at 37 ℃ for 48 h in the oscillators. Then specimens in each group were randomly assigned into three subgroups (n=8) as follows: immediate control subgroup, aging subgroups with artificial saliva storage for 6 months and 12 months. Microtensile bond strength (μTBS), fracture mode, bonding interface morphology and nanoleakage were evaluated.@*Results@#Immediately and with artificial saliva storage for 6 months and 12 mouths, the μTBS of TRY group ([49.04±3.57], [37.01±3.21] and [35.27±3.56] MPa) were significantly higher than those in the control group ([40.71±3.32], [28.87±2.34] and [24.20±2.07] MPa) (P<0.05). The immediate μTBS of C-ABC group ([32.94±2.45] MPa) was significantly lower than that of the control group (P<0.05). While with artificial saliva storage for 6 months and 12 mouths, the μTBS of C-ABC group ([26.46±2.45] and [22.50±2.58] MPa) were no differences with those of the control group (P>0.05). The ratio of cohesive fracture increased with the extension of aging time. Some narrow gaps were found in hybrid layer of the control group with artificial saliva storage for 6 months and 12 mouths.@*Conclusions@#Removal of PG increased the μTBS and durable bonds to dentin, while removal of GAG decreased the μTBS, however, it can be of help to create more durable bonds to dentin.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential effect of proteoglycan (PG) and glycosaminoglycan (GAG) on the bonding of etch and rinse adhesive to dentin, in order to improve the bonding effect of dentin.</p><p><b>METHODS</b>Forty-two extracted molars were used to obtain standard dentin bonding surface, and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random numbers. Then the three groups undergone different incubating procedures as follow: specimens in chondroitinase ABC (C-ABC) group were incubated with C-ABC at 37 °C for 48 h in vibrator. Specimens in trypsin (TRY) group were incubated with trypsin, and specimens in the control group were incubated with deionized water for 48 h in the oscillators. Then specimens in each group were randomly assigned into two subgroups, A (Adper(TM) Single Bond 2) and B (Prime & Bond NT) (n = 7). The microtensile bond strength (µTBS), fracture mode and bonding interface morphology of the specimens were evaluated via microtensile testing, stereo microscope and field emission scanning electron microscopy (FE-SEM) respectively after specimens being incubated in 37 °C water for 24 h.</p><p><b>RESULTS</b>The immediate µTBS of C-ABC group bonding with adhesive A and B [(32.9±2.5) and (26.8±2.2) MPa] were significantly lower than that of the control group [(40.7±3.3) and (34.6±3.7) MPa] (P < 0.05). While the immediate µTBS of TRY group [(49.0 ± 3.6) and (44.5 ± 3.0) MPa] were significantly higher than that of the control group(P < 0.05).</p><p><b>CONCLUSIONS</b>Dentin PG participates in the dentin bonding process. Removal of PG increased the immediate µTBS of dentin and total etching adhesives, while removal of GAG decreased the immediate µTBS.</p>
Subject(s)
Humans , Acid Etching, Dental , Methods , Bisphenol A-Glycidyl Methacrylate , Pharmacology , Chondroitin ABC Lyase , Pharmacology , Dental Bonding , Dental Stress Analysis , Dentin , Chemistry , Dentin-Bonding Agents , Pharmacology , Glycosaminoglycans , Pharmacology , Hot Temperature , Phosphoric Acids , Polymethacrylic Acids , Proteoglycans , Pharmacology , Random Allocation , Resin Cements , Tensile Strength , Trypsin , PharmacologyABSTRACT
Objective To evaluate the efficacy and safety of DanHong injection in treatment of diabetic nephropathy.Methods 80 patients with diabetic nephropathy were selected and divided into two groups randomly after admission;40 cases for the observation group,received Danhong injection treatment ;40 cases as control group treated with losartan potassium tablets.Two treatment situations and adverse reactions were assessed after 4wk treatment.Results Effective rate in observation group was 50% significantly higher than that in control group of 27.5% (P<0.05,see Table 1);During treatment period both groups had no obvious adverse reactions,all patients can be tolerated.Conclusion Danhong injection for treatment of diabetic nephropathy was not only good,but also relatively safe.