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Objective:To investigate the effect of doctor-nurse-patient collaborative nursing mode on long-term self-management ability and cardiovascular events of patients after percutaneous coronary intervention (PCI).Methods:Totally 160 patients who received PCI from the department of cardiology of the First Affiliated Hospital of Chengdu Medical College were randomly assigned to control group ( n=80) and experimental group ( n=80). The traditional nursing model was adopted in the control group. However, the nursing mode of doctor-nurse-patient collaborative was adopted in experimental group. The self-management ability and cardiovascular events in patients after PCI at 6 months and 12 months in two groups were both measured by chronic disease management software, self-behavioral management scale, Minnesota Living Scale, self-made medical care work assessment scale and self-made satisfaction questionnaire. Results:The rates of adverse habits management, symptom management, emotional cognition, emergency management, disease knowledge management, general life management, and therapy compliance management in the experimental groups, 6 months after operation, were 96.3%(77/80), 73.8%(59/80), 80.0%(64/80), 91.3%(73/80), 85.0%(68/80), 81.3%(65/80), respectively. In the control group, the above indexes were 85.0%(68/80), 56.3%(45/80), 62.5%(50/80), 75%(60/80), 75%(60/80), 65%(52/80). The difference between the two groups was statistically significant ( χ 2 value was 5.375-7.530, P<0.05). In the experimental group, 12 months after PCI, the rates of the above indexes were 91.3%(73/80), 78.8%(63/80), 82.5%(66/80), 93.8% (75/80), 87.5%(70/80), 86.3%(69/80), 93.8%(75/80). While, the ratios in the control group were 77.5%(62/80), 52.5%(42/80), 66.3%(53/80), 78.8%(63/80), 66.3%(53/80), 65.0%(52/80), 75.0%(60/80), there was significantly difference between two groups ( χ 2 value was 5.542-12.218, P<0.05). Furthermore, there were 12 and 38 cases of cardiovascular events at 6 months and 12 months after operation, and 28 and 60 cases in the control group, respectively. Meanwhile, the number of re-hospitalizations events in experimental group were 13, and 27 in the control group, after 12 months operation, there was significantly difference between two groups( χ 2 value was 8.533,12.745, 6.533, P<0.05). The quality of life scores and medical work quality score in the experimental group in different months were 31.26±8.39 (6 months), 26.39±4.76 (12 months), 77.44±8.21 (6 months) and 71.50±5.73(12 months), respectively. However, the scores in the control group were 40.51±8.38, 35.39±4.89, 65.90±5.62, 63.55±5.62, there was significant difference between two groups ( t value was -10.373-11.804, P<0.01). And the overall satisfaction of patients in the experimental group [96.3%(77/80)] was significantly higher than the control group [85.0% (68/80)]. The difference between the two groups was statistically significant ( χ 2 value was 5.959, P<0.05). Conclusion:The doctor-nurse-patient collaborative nursing mode can improve the long-term self-management ability, reduce the occurrence of cardiovascular events and the number of re-hospitalizations in patients after PCI, and improve the quality of life and satisfaction of patients.
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Objective To investigate the correlation between circulating uncoupling protein 2(UCP2) level and severity of coronary artery disease(including Gensini score and criminal vessel counts)in patients with stable coronary artery disease(SCAD),and to analyze the predictive value of circulating UCP2 and urine acid (UA)for SCAD. Methods Three hundred and thirty patients from June 2015 to June 2017 were enrolled. Two hundred and forty patients with SCAD(SCAD group),90 patients without coronary artery disease(control group) were diagnosed. The circulating UCP2 level was detected by enzyme linked immunosorbent assay (ELISA) sandwich method. Results The levels of circulating UCP2 and UA in SCAD group were higher than those in the control group(UCP2[1.60(0.67,4.60)ng/mL]vs.[0.42(0.28,0.59)ng/mL](P<0.01),UA[(365.74 ± 66.06) μmol/L] vs. [(268.11 ± 45.81)μmol/L],P < 0.01). Multivariate logistic regression analysis showed that UCP2 (OR = 1.010 ,95% CI :1.001 ~ 1.020 ,P = 0.025)and UA(OR = 1.039 ,95% CI :1.007 ~ 1.072 ,P < 0.05)were independently associated with SCAD. Correlation analysis showed that the circulating UCP2 level was positively correlated with Gensini score(r=0.780,P<0.01)and criminal vessel counts(r=0.543,P<0.01). The receiver operating characteristic curve(ROC)showed that the optimal cutoff point of the circulating UCP2 level predicting SCAD was 0.64 ng/mL,and the sensitivity was 0.833 and the specificity was 0.944. No significant difference was observed in area under the curve between circulating UCP2 and UA(ΔAUC). Conclusion The high circulating UCP2 level indicates more severe coronary lesions in patients with SCAD. Circulating UCP2 level may be a new indicator of predicting SCAD,equal to the traditional oxidative stress related indicator of serum UA.
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Objective To discuss the correlation between serum uric acid (SUA) level and the severity of coronary borderline lesions in acute coronary syndrome (ACS) and the prognosis of percutaneous coronary intervention (PCI).Methods The 192 patients who were diagnosed as coronary borderline lesions in ACS and PCI were measured.All Patients were divided into two groups:hyperuricemia group and normal SUA group.The severity of coronary arterial lesions and flow in PCI was compared.The cardiac function changes and incidence of major adverse cardiovascular events (MACE) were recorded.Results The results of correlation analysis showed the positive correlation of SUA level and Gensini score (r =0.710,P ＜ 0.05).Patients with hyperuricemia group had a significantly higher prevalence of no-reflow on angiography,in-hospital and six-month prevalence of MACEs compared with the normal SUA group (P ＜ 0.05).The UA on admission has an independent association with coronary slow flow following primary PCI and in-hospital MACEs among patients with ACS (P ＜ 0.05).Conclusion The patients has higher the level of SUA of ACS borderline lesions patients will has more serious the coronary arterial lesions.The risk of coronary slow flow increased in the patients complicated with hyperuricemia who underwent PCI.
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Objective To investigate the effect of Salvianolic Acid B(Sal B)on vascular function of db/db mice and reveal the potential mechanism. Methods 20 male db/db mice were divided into 2 groups,the con-trol group(n=10)and Sal B group(n=10). 10 age-matched male C57BL/KsJ mice were used as the wild type control. Mice in Sal B group were given Sal B ,100 mg/(kg · d)by tube. Mice in db/db control group and in wild type control were given the same volume of saline. Body weight,tail blood pressure,heart rate and fasting blood glucose level were measured every week. After 6-weeks treatment ,thoracic aorta was obtained and used to detect the levels ofsuperoxide anion and NO,vascular function,eNOS,p-eNOS,AMPK and p-AMPK. Results Sal B could reduce the body weight and fasting blood glucose level of db/db mice ,but had no effect on blood pressure. Sal B could decrease the level of superoxide inon,increased NO level,and improved endothelium-dependent but not endothelium- independent diastolic function. Sal B could increase phosphorylation levels of eNOS and AMPK. Conclusion Sal B can reduce the oxidative stress ,increases NO level in vasculature ,and improves the endo-thelium-dependent vasodilation in the diabetic mice ,which may be associated with the promotion of AMPK phos-phorylation.
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Objective To investigate the effect of Salvianolic Acid B(Sal B)on vascular function of db/db mice and reveal the potential mechanism. Methods 20 male db/db mice were divided into 2 groups,the con-trol group(n=10)and Sal B group(n=10). 10 age-matched male C57BL/KsJ mice were used as the wild type control. Mice in Sal B group were given Sal B ,100 mg/(kg · d)by tube. Mice in db/db control group and in wild type control were given the same volume of saline. Body weight,tail blood pressure,heart rate and fasting blood glucose level were measured every week. After 6-weeks treatment ,thoracic aorta was obtained and used to detect the levels ofsuperoxide anion and NO,vascular function,eNOS,p-eNOS,AMPK and p-AMPK. Results Sal B could reduce the body weight and fasting blood glucose level of db/db mice ,but had no effect on blood pressure. Sal B could decrease the level of superoxide inon,increased NO level,and improved endothelium-dependent but not endothelium- independent diastolic function. Sal B could increase phosphorylation levels of eNOS and AMPK. Conclusion Sal B can reduce the oxidative stress ,increases NO level in vasculature ,and improves the endo-thelium-dependent vasodilation in the diabetic mice ,which may be associated with the promotion of AMPK phos-phorylation.
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AIM:To explore the effects of PPARγon the elevated level of reactive oxygen species ( ROS) in-duced by high glucose and its mechanism .METHODS:Human umbilical vein endothelial cells ( HUVECs) were cultured with DMEM containing high glucose (33 mmol/L D-glucose), and DMEM containing lower glucose (5.5 mmol/L D-glu-cose) was used as control .Superoxide anion and nitric oxide fluorescence probes were used to observe the effects of PPAR γagonist on ROS and NO productions in the HUVECs .The uncoupling protein 2 (UCP2) protein level in the HUVECs was detected by Western blotting .RESULTS:PPARγagonist pioglitazone inhibited the ROS generation and prevented the de-crease in NO level under high glucose condition , and these effects were reversed by pretreatment with PPARγantagonist GW9662.The results of Western blotting indicated that PPARγagonist pioglitazone up-regulated the UCP2 expression un-der high glucose condition , and this effect was also blocked by GW 9662.Inhibition of UCP2 by genipin attenuated the effect of pioglotazone on the ROS production .CONCLUSION: Activation of PPARγinhibits ROS generation under high glucose condition , and this effect may mediate by up-regulation of UCP2.
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Objective To explore the effects of one time loading of different doses of simvastatin before percutaneous coronary intervention (PCI)on post-PCI inflammation,oxidation stress and the endothelium function in (ACS) patients. Methods Totally 124 cases with ACS were randomly divided into two groups:high dose simvastatin group(40 mg,62 cases),low dose simvastatin group (20 mg,62 cases). Each group was given the same basic treatment. Blood samples were obtained from all the patients before and 12 h after PCI,and endothelin-1 (ET-1),nitric oxide (NO),interleukin-10(IL-10),high sensitive - C reactive protein(hs-CRP),superoxide dismutase (SOD) and malondialdehyde(MDA) were detected. Results The baseline information,distribution of sex,age,and implanted frames had no significant differences between the two groups (P＞0.05).Before PCI,the levels of ET-1,NO,IL-10,hs-CRP,SOD and MDA had no significant differences (P＞0.05) between the two groups.After PCI,ET-1,IL-10 and hs-CRP levels in simvastatin 40 mg group were significant lower while NO level was higher than in simvastatin 20 mg group[(4.4 ± 1.1)ng/L vs.(4.8±1.2)ng/L,t=2.03,P=0.044; (15.0±6.3) ng/L vs.(18.7±9.0)ng/L,t=2.68,P=0.008;(26.9±10.0)ng/L vs.(31.5± 11.7)ng/L,t=2.52,P =0.022;(51.9± 10.9)μmol/L vs.(47.1±11.8)μmol/L,t=2.37,P=0.020].There were no significant differences in MDA and SOD levels between the two groups.For safety,all the patients had no abnormality in liver and kidney function after treatment. Conclusions Compared with 20 mg simvastatin loading before PCI,the 40mg simvastatin loading may decrease the inflammatory cytokines and improve the endothelium function more effectively.
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Transient receptor potential vanilloid type-1 channel (TRPV1) is a non-selective cation channel with a preference for calcium ions that is able to sense a vast range of endogenous physical and chemical stimuli and plays an important role in transducing the sensations of noxious heat and pain signaling. Recent studies showed that TRPV1 is widely expressed in different tissues and organs beyond the sensory nerves and has multiple biological effects that are involved in functional regulation in the pancreas, blood vessel, adipose tissue and liver. To further understand the link between TRPV1 and cardiometabolic diseases, we reviewed the role of TRPV1 in hypertension, diabetes, obesity, and dyslipidemia. This review provides new insights into the involvement of TRPV1 channels in the pathogenesis of cardiometabolic disorders and implicates this channel as a potential therapeutic target for the management of cardiometabolic diseases.
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Adipose Tissue , Aluminum Hydroxide , Blood Vessels , Calcium , Capsaicin , Carbonates , Dyslipidemias , Glycosaminoglycans , Hot Temperature , Hypertension , Ions , Liver , Obesity , Pancreas , SensationABSTRACT
【Objective】To observe the effect of Ma Xing Shi Gan Decoction(MXSGD)on metabolism of oxygen free radicals in mice with bronchial asthma.【Methods】Forty BALB/c mice were randomized into 4 groups:normal group,model group,low-dose MXSGD group(20?g?kg~(-1)?d~(-1))and high-dose MXSGD group (80?g?kg~(-1)?d~(-1)).Except the normal group,the mice in other 3 groups received sensitization by intraperitoneal injection of ovalbumin(OVA)and challenge by inhalation of OVA to induce bronchial asthma.Meanwhile,MXSGD groups were given the corresponding doses according to the experimental design by gastric gavage,and normal group and model group received the same volume of normal saline for 27 days.Twelve-four hours after last challenge,blood sample was taken and homogenate of lung tissue was prepared for the detection of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)contents in the plasma and the lung tissue.【Results】High-and low-dose MXSGD increased SOD and GSH-Px contents and decrease MDA content in the lung tissue and plasma(P
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Objective To observe the changes of chief chemical components(hypaconitine,ephedrine,pseudoephedrine,peoniflorin,glycyrrhetic acid) in the combinations of different-dose herbs of Wutou Decoction(WD). Methods Uniform design method was adopted to design the experiments,and HPLC was applied to determine the contents of chief chemical components of hypaconitine,ephedrine,pseudoephedrine,peoniflorin,glycyrrhetic acid. DPS V7.55 was used to carry out the nonlinear regression analysis of experimental data such as decocting rates of the chief chemical components. The mathematical model was established to analyze the experimental results. Finally,with the decocting rates of the chief chemical components as the optimizing indexes,the combination of optimal-dose herbs of WD was seeked. Results There existed an interaction between each herb of WD,and each herbs as well as the interaction between the herbs had a certain effects on the decocting rate of the chemical components. In particular,the interaction of prepared Radix Aconiti and prepared Radix Glycyrrhizae had obvious effects on the decocting rates of hypaconitine and glycyrrhetic acid. On the basis of the different optimization index,different combinations of optimal-dose herbs of WD can be obtained. Conclusion Through the method of nonlinear regression analysis,rich connotation of compatibility in Chinese herbal formula can be obtained.The optimization of optimal-dose herbs combination of the formula should be based on the pharmacological actions.
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Objective To determine the contents of hypaconitine and mesaconitine in Shubining Granules. Methods The HPLC method was established for the determin ation of hypaconitine and mesaconitine in Shubining Granules. The HPLC was perfo rmed on Akasil C18(4.6 mm?250 mm,5 ?m) column,mobile phase consisted of meth anol-0.1 %triethylamine (70 ∶30),column temperature 30 ℃,and the flow rare was 1.0 mL/min.The detection wavelength was 230 nm.Results A good linearity w as obtained in the range of 0.126～2.520 ?g of Hypaconitine and 0.152～3.04 ?g of Mesaconitine.The average recovery of Hypaconitine was 101.12 %.The average recovery of Mesaconitine was 99.68 %. Conclusion This method is simple and qui ck,and can be used as quality control of Shubining Granules.
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Objective To determine the contents of strychnine and brucine in Xiaobining Spray.Methods A HPLC method was adopted and the chromatogram conditions were as follows:column of Kromasil C18(4.6 mm? 250 mm,5 ? m),mobile phase consisting of acetonitrile-0.01 mol/L sodium heptanesulfonate and 0.02 mol/L potassium dihydrogen phosphate equivalent volume mixture(10 % phosphoric acid adjusting pH to 2.8)(21:79),column temperature of 30 ℃,flow rate of 1.0 mL/min,detection wavelength of 260 nm.Results A good linearity was obtained form 0.109 2 ? g to 0.546 0 ? g for brucine(r=0.999 6)and 0.173 6 ? g to 0.868 0 ? g for strychnine(r=0.999 7).The average recovery of brucine was 100.59 % with RSD being 1.81 %,and of strychnine was 100.83 % with RSD being 1.69 % .Conclusion This method is simple,reproducible,and can be used to determine the strychnine and brucine in Xiaobining Spray.