ABSTRACT
Objective: To establish a method for the quality standard of Biyankang granule. Methods: Scutellaria baicalensis Georgi and Puerariae Iobatae radix were identified by TLC. The content of baicalin was determined by HPLC with a Dubhe C18 column (250 mm × 4. 6 mm, 5 mm). The mobile phase was methanol and 0. 4% phosphoric acid with gradient elution. The flow rate was 1. 0 ml· min-1 and the detection wavelength was 280 nm. The column temperature was 30℃ and the injection volume was 10 ml. Re-sults:The spots in TLC were clear without any interference. A good linear relationship was shown within the range of 15. 02-300. 43 m g·ml-1 for baicalin (r=1.000 0). The average recovery of baicalin was 102.62% (RSD =0.72%, n =6). Conclusion: The method is specific with high accuracy and good reproducibility, which can be used for the quality control of Biyankang granule.