ABSTRACT
To analyze the clinical significance of COX-2, GLUT-1 and VEGF expressions in endometrial cancer tissues. One hundred and eight tissue samples from the patients with endometrial cancer enrolled in our hospital from August 2011 to July 2014 were selected, including 60 normal tissue samples [normal group], 60 neoplastic tissue samples [neoplastic group] and 60 cancer tissue samples [cancer group]. All the samples were subjected to immunohistochemical assay to detect the expressions of COX-2, GLUT-1 and VEGF. The clinical data were also investigated for correlation analysis. The positive rates of COX-2 in normal group, neoplastic group and cancer groups were 3.3%, 21.7% and 55.0% respectively. The positive rates of GLUT-1 in normal group, neoplastic group and cancer groups were 3.3%, 25.0% and 70.0% respectively. The positive rates of VEGF in normal group, neoplastic group and cancer groups were 1.7%, 23.3% and 63.3% respectively. With increasing stage of such cancer, decreasing degree of differentiation and lymphatic metastasis, the positive expression rates of COX-2, GLUT-1 and VEGF proteins were raised significantly [P<0.05]. Spearman's correlation analysis showed that the expressions of COX-2 and GLUT-1 [r=0.207, P<0.05], COX-2 and VEGF [r=0.243, P<0.05], as well as GLUT-1 and VEGF [r=0.758, P<0.05] were positively correlated. COX-2, GLUT-1 and VEGF were highly prominent in endometrial cancer, especially in the patients with low degree of differentiation, late stage and metastasis. They functioned synergistically in the onset and progression of this cancer
Subject(s)
Humans , Female , Cyclooxygenase 2 , Glucose Transporter Type 1 , Vascular Endothelial Growth Factor AABSTRACT
Objective To study on the establishment of human ovarian cancer models with nude mice which can be investigated efficiently.MethodsHuman epithelial ovarian cancer 3AO cells cultured in vitro was injected by S.C.and I.P.in nude mice,so as to establish and observe human epithelial ovarian carcinoma models in nude mice.When the transplanted tumor grew big enough,the speciments of transplanted tumors were identified by histological and electron microscope observation and DNA content was analysed by flow cytometry.ResultsThe human ovarian cancer models with nude mice have been established successfully.There were many differences between the two kinds of models.The success rate of transplantation subcutaneously was 93.3%(14/15),whereas a little shorter rate was 86.7%(12/15) in transplantation peritoneally.ConclusionHuman ovarian cancer models with nude mice are easy to establish.They could display the invasive growth behavior and metastasis as same as human ovarian carcinoma.They are good models for experimental research of ovarian carcinoma.