ABSTRACT
Objective To study the quality control method of Feireqingjie Oral Liquid. Methods UV was used to determine the content of total flavonoid. Cortex Mori was identified qualitatively by TLC. Pheretima was identified qualitatively by PC. Results Total flavonoid showed a good linear relationship in the range of 0.005 26-0.052 60 mg/mL, r=0.999 8. The average recovery rate was 98.65%, RSD=0.28% (n=6). The TLC spot of Cortex Mori was coincident with the standard reference, and Pheretima showed the same color as the reference in PC, with no interference of negative control. Conclusion The method is convenient, accurate and can be used as one of the quality control methods of Feireqingjie Oral Liquid.
ABSTRACT
Poor stability existed in the anaphase of the high-cell-density fermentation of Saccharomyces crevisiae for S-adenosyl-L-methionine (SAM) production in 5 L fermentor. To improve the fermentation stability, we studied the addition of diammonium hydrogen phosphate, sodium glutamate and adenosine disodium triphosphate into glucose feeding solution. Study of four fed-batch cultures showed that, after 34 h fermentation, when dry cell weight reached 100 g/L, the addition of 50 g pre-L-methionine and glucose feeding with 10 g/L adenosine disodium triphosphate was optimal for SAM production. Under this condition, after 65.7 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 180 g/L and 17.1 g/L respectively.
Subject(s)
Adenosine Triphosphate , Pharmacology , Fermentation , Phosphates , Pharmacology , S-Adenosylmethionine , Genetics , Saccharomyces cerevisiae , Genetics , Sodium Glutamate , PharmacologyABSTRACT
OBJECTIVE:To conduct a pharmacognostical study of Rosa laevigata so as to provide scientific evidence for clinical application and development of this herbal plant. METHODS:The pharmacognosy of R.laevigata was studied from aspects of its plant resource,characteristics,microscopic and physiochemical identifications,etc. RESULTS:The R.laevigata was distinctive in plant resource,characteristics,and microscopic and physiochemical identifications. CONCLUSION:The results of the study serve as scientific evidences for clinical application and development of R.laevigata.