Resistance training combined with athletic training / Treinamento de resistência associado ao treino de atletismo / Entrenamiento de resistencia asociado al entrenamiento de atletismo

ABSTRACT Introduction Athletics has high requirements for resistance and strength. Therefore, more and more researchers are focusing on improving these athletes' endurance and muscular strength, prioritizing their sports performance. Objective Explore methods of adding resistance to conventional training to increase performance and muscle strength in track and field. Methods Volunteers in the experiment and control groups were randomly assigned. Both groups received 10 weeks of physical training. The experimental group used a 10-meter lap run on the track, with squats, left and right-side squat jumps, fast bow squats, fast squats, fast trot runs, and lower limb lifting activities. The control group used only running in their conventional training. Results Significant differences were found in the blood lactate test rates of the experimental groups. The maximum heart rate and heart rate at 3 minutes after the end of the test decreased, the recovery rate increased, the number of continuous squats and average squats in one minute increased significantly, the 8RM squat, 1RM squat, vertical jump. Long jump had very significant differences, including the vertical stiffness in the drop jump. Conclusion The endurance and muscle strength of track and field athletes had positive impacts through the experimental training presented. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução O atletismo tem elevados requisitos para resistência e força. Portanto, cada vez mais pesquisadores direcionam o foco da pesquisa ao aprimoramento da resistência e da força muscular desses atletas, priorizando o seu desempenho esportivo. Objetivo Explorar métodos com a adição de treinamento de resistência ao treino convencional visando aumentar o desempenho e a força muscular no esporte de atletismo. Métodos Os voluntários dos grupos experiência e controle foram designados aleatoriamente. Ambos os grupos receberam 10 semanas de treinamento físico. O grupo experimental utilizou corrida de 10 metros de volta na pista, com agachamentos, salto de agachamento lateral à esquerda e à direita, agachamento rápido de proa, agachamento rápido, corrida rápida de trote e atividades de elevação de membros inferiores. O grupo de controle utilizou apenas a corrida em seu treino convencional. Resultados Foram encontradas diferenças significativas nos índices de teste de lactato no sangue dos grupos experimentais. A frequência cardíaca máxima e a frequência cardíaca aos 3 minutos após o final do teste diminuíram, a taxa de recuperação aumentou, o número de agachamentos contínuos e média de agachamentos em um minuto aumentou significativamente, o agachamento de 8RM, agachamento de 1RM, salto vertical e salto em posição longa tiveram diferenças muito significativas, incluindo a rigidez vertical no salto de queda. Conclusão A resistência e a força muscular dos praticantes de atletismo tiveram impactos positivos através do treinamento experimental apresentado. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción El atletismo tiene altas exigencias de resistencia y fuerza. Por ello, cada vez más investigadores dirigen el foco de la investigación a la mejora de la resistencia y la fuerza muscular de estos atletas, priorizando su rendimiento deportivo. Objetivo Explorar métodos con la adición de entrenamiento de resistencia al entrenamiento convencional con el fin de aumentar el rendimiento y la fuerza muscular en el deporte del atletismo. Métodos Se asignaron aleatoriamente voluntarios de los grupos experimental y de control. Ambos grupos recibieron 10 semanas de entrenamiento físico. El grupo experimental utilizó la carrera de 10 metros en la pista, con sentadillas, salto en cuclillas a la izquierda y a la derecha, sentadilla rápida, carrera de trote rápido y actividades de elevación de las extremidades inferiores. El grupo de control sólo utilizó la carrera en su entrenamiento convencional. Resultados Se encontraron diferencias significativas en las tasas de prueba de lactato en sangre de los grupos experimentales. La frecuencia cardíaca máxima y la frecuencia cardíaca a los 3 minutos de terminar la prueba disminuyeron, la tasa de recuperación aumentó, el número de sentadillas continuas y el promedio de sentadillas en un minuto aumentaron significativamente, la sentadilla 8RM, la sentadilla 1RM, el salto vertical y el salto de longitud tuvieron diferencias muy significativas, incluyendo la rigidez vertical en el salto de caída. Conclusión La resistencia y la fuerza muscular de los practicantes de atletismo tuvieron impactos positivos a través del entrenamiento experimental presentado. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Traditional Chinese Medicine Treatment of Psoriasis by Regulating microRNA： A Review / 中国实验方剂学杂志

The incidence of psoriasis which is characterized by dryness， scaling and itching of the skin has been on the rise. It can have a profound psychological impact on patients' quality of life. Accumulating research has been conducted on the mechanisms of psoriasis in western medicine， from the difference of pathological manifestations of terminal keratinocytes， the disorder of expression of related factors and cells， to the immune imbalance of T lymphocytes and their subsets， or the abnormal transcription dominated by genetic genes. As a result， a complex and huge mechanism network has formed and many hypotheses have emerged. In recent years， with the in-depth research on non-coding genes， it has been clarified that microRNA-modified multi-pathway effect interferes with the occurrence and development of psoriasis and affects the proliferation， apoptosis， and differentiation of keratinocytes. The research on microRNA involves both genetics and immunology， which can help improve the key links in the micro pathway of psoriasis. Thus， it is a key part in the pathogenesis of psoriasis and has also become the hotspot and difficulty of modern research on psoriasis. At the same time， we should give full play to the advantages of traditional Chinese medicine （TCM） in syndrome differentiation and treatment. To be specific， microRNA targets of compound Chinese medicine preparations with the functions of clearing heat， cooling blood， detoxifying and removing blood stasis or effective medicinal monomers such as paeonol， tripterygium glycosides， shikonin， curcumin， total glucosides of paeony and indirubin should be explored， and microRNA can be used as the basis for blood syndrome differentiation of psoriasis. Thereby， the syndrome differentiation theory of TCM and micro indicators of western medicine are integrated， to make full use of characteristics of TCM and guide the clinical syndrome differentiation and treatment. At present， the intervention on microRNA in TCM is rarely studied， and available studies mainly focus on several targets such as microRNA-155， microRNA-210， microRNA-21， microRNA-203， microRNA-320， microRNA-124， microRNA-330， microRNA-146a， and microRNA-15a-5p. This paper summarizes the research on compound Chinese medicine prescriptions and monomers in the treatment and syndrome differentiation of psoriasis through the intervention of microRNA， which is expected to provide a reference for the research on psoriasis in TCM and western medicine and the establishment of microRNA-based database.

Follicular Helper T Cells， Psoriasis， and Chinese Medicine Intervention： A Review / 中国实验方剂学杂志

Follicular helper T （Tfh） cells are a newly discovered subset of CD4+ T cells. As reported， abnormalities in their development， differentiation， and function are closely related to the occurrence of autoimmune diseases. Psoriasis is an autoimmune skin disease and it is intractable with a prolonged course. At present， it is generally believed that immune imbalance mediated by T cells is the core mechanism of the pathogenesis of psoriasis. In the context of this mechanism， Tfh cells are associated with psoriasis， and their cellular level and abnormal expression of related candidates can promote the occurrence of psoriasis. In terms of treatment， Chinese medicine， by virtue of the characteristics of wide application and low price， serves as a good complementary and alternative treatment option for psoriasis. As confirmed by previous findings， some active ingredients or preparations of Chinese medicine used in the treatment of psoriasis can also intervene in and regulate the immune response mediated by Tfh cells and the related candidates. Based on the research reports and experimental data， the present study reviewed the research progress from the differentiation of Tfh cells， the relationship between Tfh cells and psoriasis， and the intervention and regulation of Tfh cells and related molecules by Chinese medicine， which is expected to provide certain theoretical support and references for the determination of new strategies for psoriasis treatment and research in related fields.

Clinical application of the thumb-tack needle for subcutaneous embedding therapy on treating insomnia with insufficiency of heart and spleen among breast cancer patients / 中国实用护理杂志

Objective@#To explore the clinical therapeutic method and effect of the thumb-tack needle for subcutaneous embedding therapy on treating insomnia with insufficiency of heart and spleen among breast cancer patients.@*Methods@#Totally 80 breast cancer patients with insomnia with insufficiency of heart and spleen treated in department of oncology, the Second Affiliated Hospital of Xi′an Jiao Tong University from March 2018 till November 2018 were selected and assigned into an experiment group and a control group by random number table, 40 cases in each one. The experiment group applied thumb-tack needle for subcutaneous embedding therapy, selecting Shenmen, Sanyinjiao, Zusanli, Anmian as major acupoint and Taibai as assistant acupoint. The control group used fake needles at the same places. After four-week treatment, we evaluated the therapeutic effect using the Pittsburgh Sleep Quality Index (PSQI) and observed side effects.@*Results@#Before treatment, no significantly statistical differences were observed between the two groups on global and each dimension′s PSQI scores (P>0.05). After treatment, the PSQI scores and PSQI global score of the experiment group on sleep quality, sleep latency, sleep duration, sleep efficiency, sleep disturbances, and daytime dysfunction were (1.58±0.53), (1.72±0.33), (1.57±0.64), (1.61±0.68), (1.05±0.42), (1.27±0.53), (8.91±2.73) points respectively, which were (1.86±0.71), (1.90±0.41), (1.85±0.47), (1.97±0.83), (1.42±0.91), (1.56±0.45), (10.86±4.53) points respectively in the control group. There was significant difference between the two groups on PSQI global scores (t=1.998-2.637, P<0.05). The number of patients in the experiment group assessed as clinical cure, obviously effective, effective and noneffective were 8, 13, 9, 10 respectively, which were 1, 5, 12, 22 in the control group respectively. The clinical efficacy showed significant difference between the two groups (χ2=27.68, P<0.01). No side effects, such as infection, needle drop-off and etc. were observed.@*Conclusions@#The thumb-tack needle for subcutaneous embedding therapy could improve sleep quality of breast cancer patients with insomnia with insufficiency of heart and spleen, and it′s easy to operate, safe with little side effects.

Application of bedside color Doppler ultrasound-guided popliteal vein catheter thrombolytic therapy in treatment of lower extremity deep venous thrombosis / 中国介入影像与治疗学

Objective To evaluate the feasibility of beside color Doppler ultrasound-guided popliteal vein catheter in treatment of lower extremity deep venous thrombosis (LEDVT).Methods Data of 50 patients with unilateral LEDVT were reviewed,including 25 cases underwent beside color Doppler ultrasound-guided catheter thrombolytic therapy (catheter thrombolytic group) and 25 cases underwent systemic thrombolysis and anticoagulant therapy (anticoagulant thrombolysis group).The cure rate,hospitalization time and the bilateral diameter differences were compared between the 2 groups.Results Thrombolytic effective rate was 100％ in both the 2 groups.Thirty patients were clinical cured,including 22 cases in catheter thrombolytic group and 8 cases in anticoagulant thrombolysis group.The cure rates were significantly different between the 2 groups (88.00％ [22/25] vs 32.00％ [8/25],x2 =16.333,P＜ 0.001).The hospitalization time of catheter thrombolytic group ([12.32 ± 1.49] days) was shorter than that of anticoagulant thrombolysis group ([16.44±2.95]days;t=-6.426,P＜0.001).The diameter differences between LEDVT side and contralateral side of the upper and lower 15 cm form knees in catheter thrombolytic group showed no statistical difference compared with anticoagulant thrombolysis group before the treatment (both P＞0.05),while of catheter thrombolytic group were smaller than those of anticoagulant thrombolysis group after the treatment (both P＜0.05).And the bilateral diameter differences before the treatment were larger than those after the treatment in both groups (all P ＜ 0.05).Conclusion Both of beside color Doppler ultrasound-guided catheter thrombolytic therapy and systemic thrombolysis and anticoagulant therapy can be used to treat LEDVT,while the efficacy of ultrasound-guided catheter thrombolytic therapy is better.

Value of prenatal ultrasonography in diagnosis of Pentalogy of Cantrell / 中华全科医师杂志

Four cases of Pentalogy of Cantrell were diagnosed by prenatal ultrasonography and confirmed by autopsy after labor induction.There were 1 case of complete Pentalogy of Cantrell and 3 cases of incomplete Pentalogy.In 3 cases of incomplete Pentalogy of Cantrell,1 case presented with high umbilical cord prolapse,extraspectoral heart,partial defect of the lower part of the sternum,partial defect of the pericardium,single umbilical artery and cervical lymphatic cyst;1 case presented with high umbilical cord prolapse,extraspectoral heart,thoracic fissure and ventricular septal defect;and 1 case presented with umbilical cord,extraspectoral heart,partial inferior sternal segment defect,ventricular septal defect and bilateral clubfoot varus.In l case of complete Pentalogy of Cantrell,there were high umbilical cord bulge,extraspectoral heart,chest fissure and inferior segment defect,anterior diaphragm and pericardium defect,total forebrain deformity,right 12th ribs loss and ventricular septal defect.Ectopic heart and omphalocele are typical echographic features of Pentalogy of Cantrel and prenatal ultrasonography is of important value in early diagnosis of this congenital malformation.

Ultrasound measurement of congenital cystic adenomatoid malformation volume ratio in prenatal evaluation on prognosis in fetus with congenital pulmonary sequestrations / 中国介入影像与治疗学

Objective To investigate the value of cystic adenomatoid malformation volume ratio (CVR) using prenatal ultrasound in evaluation of congenital pulmonary sequestrations (PS) prognosis in fetus.Methods Totally 88 cases of fetal PS diagnosed with prenatal ultrasound were enrolled.The fetal CVR were recorded,and the clinical outcomes were observed.The fetus were further divided into CVR≥1.6 group and CVR＜1.6 group,then the fetal hydrops rate,incidence of respiratory distress symptoms after birth and perinatal infant survival rate between two groups were compared.Results In 88 cases,prenatal ultrasound diagnosed intralobar pulmonary sequestration (ILS) type in 62 cases (62/88,70.45％),extralobar pulmonary sequestration (ELS) type I in 19 cases (19/88,21.59％),and ELS type Ⅱ in 7 cases (7/88,7.95％).There were 81 (81/88,92.05％) live births,5 (5/88,5.68％) of induction,and 2 (2/88,2.27％) of intrauterine fetal death,respectively.In 44 cases of CVR≥1.6 group,36 cases (36/44,81.82％) had respiratory symptoms after birth,and 39 (39/44,88.64％) had combined fetal hydrops.The live birth rate was 84.09％ (37/44).In 44 cases of CVR＜1.6 group,3 cases (3/44,6.82％) had respiratory symptoms after birth and 3 (3/44,6.82％) had combined fetal hydrops.The live birth rate was 100％ (44/44).The fetal hydrops rate,incidence of respiratory symptoms after birth and perinatal infant survival rate were statistically different between the two groups (all P＜0.05).Conclusion Prenatal ultrasound CVR is an effective index for screening and assessing the prognosis in fetus with PS.

Qufeng Tongluo Prescription () inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression / 中国结合医学杂志

<p><b>OBJECTIVE</b>To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription (, QFTL) on the regulation of mesangial cells (MCs) proliferation and apoptosis.</p><p><b>METHODS</b>The MCs used in this experiment have undergone five passages induced by lipopolysaccharide (LPS). Changes in the proliferation, apoptosis, cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium (MTT) reduction assay, flow cytometry, Western blot and quantitative real-time polymerase chain reaction (qRT-PCR), respectively.</p><p><b>RESULTS</b>The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24, 48 and 72 h, respectively (P<0.05 or P<0.01). Moreover, the inhibitory effect is more significant in the QFTL group at 48 h (P<0.05). Compared with the control group, LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases, while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase (P<0.05 or P<0.01) to cells at S phase (P<0.01), implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis, compared with the control group (P<0.05), while QFTL and Benazepril serum increased the level of MC apoptosis (P<0.01). Moreover, the difference between the QFTL group and the Benazepril group was statistically significant (P<0.01). Compared with the control group, the protein and mRNA expression levels of cylinD1, cyclin dependent kinase 2 (CDK2) and p21 were significantly increased (P<0.05 or P<0.01), p27 was decreased but with no statistical significance (P>0.05); After being treated with QFTL and Benazepril serum, the protein and mRNA expression levels of cylinD1, CDK2, p21 were decreased and p27 increased significantly (P<0.05 or P<0.01); Compared with the Benazepril group, QFTL show better effects on protein and mRNA expression levels of cylinD1, CDK2 (P<0.05 or P<0.01) and p21 protein expression (P<0.05).</p><p><b>CONCLUSION</b>QFTL inhibits MCs proliferation, promotes MCs apoptosis through an underlying mechanism of down-regulating the protein and mRNA expression levels of cylinD1, CDK2, p21 and up-regulation of the expression level of p27.</p>

Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effects of Qufengtongluo Recipe (QFTLR) on the expressions of cell cycle regulatory proteins in rat mesangial cells (MCs) in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation.</p><p><b>METHODS</b>Using the methods of serum pharmacology, we studied the expressions of cell cycle regulatory proteins in rat MCs treated with QFTLR by laser scanning confocal microscope and immunohistochemistry.</p><p><b>RESULTS</b>Compared with the normal control cells, the cells challenged with lipopolysaccharide (LPS) showed significantly enhanced expressions of cyclin D1, CDK2, and P21 (P<0.01) and obviously lowered protein expression of P27 (P<0.01). Treatment of the LPS-challenged cells with QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1, CDK2, and P21 and obvious increase of P27 expression (P<0.05 or P<0.01), but QFTLR produced stronger effects than benazepril in regulating of cyclinD1, P21 and P27 protein expressions (P<0.05 or P<0.01).</p><p><b>CONCLUSION</b>QFTLR inhibits rat MC proliferation in vitro possibly by down-regulating the cellular expressions of cyclin D1, CDK2, and P21 and up-regulating the expression of P27 protein.</p>

Effect of acupuncture on renal function and pathologic changes of kidney in rabbits with nephritis / 中国针灸

<p><b>OBJECTIVE</b>To observe the effect of acupuncture on cationized bovine serum albumin (C-BSA) nephritis model in rabbits and to explore its mechanism.</p><p><b>METHODS</b>Fifty rabbits were randomly divided into a blank group, a model group, a metoprolol group, a irbesartan group and an acupuncture group, 10 rabbits in each group. The model was established by ear vein intravenous injection with C-BSA. The positive control groups were treated by intragastric administrated with metoprolol and irbesartan, respectively. The acupuncture group was treated by acupuncture at "Fengmen" (BL 12) and "Shenshu" (BL 23). No interventions were added on the blank group and the model group. The changes of blood pressure (BP), heart rate (HR), plasma norepinephrine (NE), serum creatinine (Scr), blood urea nitrogen (BUN) and 24 hours urine protein (24 h UP) in rabbits at the time points of 3rd, 6th and 8th week of treatment were observed.</p><p><b>RESULTS</b>After the model was established, the Scr of (194.30 +/- 20.09) micromol/L, BUN of (9.19 +/- 0.66) mmol/L and 24 h UP of (277.70 +/- 20.09) mg/24 h in the model group were all higher than the Scr of (66.03 +/- 4. 76) micromol/L, BUN of (4.11 +/- 0.71) mmol/L and 24 h UP of (14.28 +/- 1. 47) mg/24 h in the blank group (all P < 0.01), and the diffused mesenteria hyperplasia and the increase of intercapillary cells in the model group were showed in the pathological sections. After 3 weeks of treatment. The Scr of (99.82 +/- 9.29) micromol/L, BUN of (6.32 +/- 0.75) mmol/L and 24 h UP of (189.67 +/- 15.45) mg/ 24 h in the acupuncture group were all decreased significantly, furthermore, the decrease of BP, HR, NE were better than the other treatment groups (P < 0.05, P < 0.01). Except the level of 24 h up and HR at 8th week, other results were as same as the 3rd week.</p><p><b>CONCLUSION</b>Acupuncture can improve the function of kidney, decrease the content of 24 h UP and the underlying therapeutic mechanism could be correlated with that acupuncture can lower excitability of sympathetic nerve and alleviate the renal pathological lesion induced by nephritis.</p>

Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice / 中国结合医学杂志

<p><b>OBJECTIVE</b>To investigate the therapeutic effects and mechanisms of using artemisinin (Art) combined with glucocorticoid (GC) to treat lupus nephritis (LN) mice.</p><p><b>METHODS</b>Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table: control group, model group, prednisone group administrated with 6.45 mg/(kg·d) prednisone suspension, and Art+prednisone group administrated with 150 mg/(kg·d) Art suspension and 3.225 mg/(kg·d) prednisone suspension. A mice model of LN was established by injection with living lymph cell suspension. The changes of urine protein/24h, the expressions of GC receptor α (GRα) mRNA, GC receptor β (GRβ) mRNA in peripheral blood mononuclear cells (PBMCs), and transcriptional coactivator P300/CBP protein in renal tissue were measured.</p><p><b>RESULTS</b>Compared with the model group, the treatment groups had significant decrease in urine protein/24 h, and renal pathological lesion (P<0.01). In the same groups, the expression of transcriptional coactivator P300/CBP protein in renal tissue and GRα mRNA were significantly increased, and GRβ mRNA expression was significantly decreased (P<0.01). And the Art+prednisone group has a better therapeutic effect than the prednisone group (P<0.01).</p><p><b>CONCLUSIONS</b>Art has therapeutic sensitization effects on GC in the LN mice. The underlying mechanism could be correlated with the effect of Art on the increase of the expressions of GRα mRNA and transcriptional coactivator P300 300/CBP protein in renal tissue and on the decrease of the expression of GRβ mRNA in PBMC.</p>

Roles of monocyte chemoattractant protein-1, RANTES and Fractalkine on promoting vulnerability of atherosclerotic plaques / 中华心血管病杂志

<p><b>OBJECTIVE</b>To elucidate the roles of monocyte chemotactic factors (MCP-1, RANTES and Fractalkine) on the vulnerability of atherosclerotic plaques in patients with stable (SAP) and unstable angina pectoris (UAP).</p><p><b>METHODS</b>Patients with SAP (n = 50) and UAP (n = 50) underwent coronary angiography (CAG) and intravenous ultrasound (IVUS) were included in the study. Monocyte chemotaxis was assayed by the transwell chamber. Concentrations of hs-CRP, MCP-1, RANTES and Fractalkine were measured by Enzyme-linked-immunosorbent assay (ELISA). mRNA expression of MCP-1, RANTES and Fractalkine in the monocytes was detected by RT-PCR.</p><p><b>RESULTS</b>IVUS evidenced soft lipid plaques in 48% UAP patients and in 16% SAP patients (P < 0.05). SAP patients had mainly fibrous and mixed plaques. Plaque burden and vascular remodeling index were significantly higher in UAP patients than in SAP patients (P < 0.01). The averaged number of migrated monocytes in the UAP patients were higher than that in patients with SAP (P < 0.01). Concentration of hs-CRP, MCP-1, RANTES and Fractalkine were significantly higher in UAP patients than those of SAP patients (P < 0.05 or P < 0.01). mRNA expression of MCP-1, RANTES and Fractalkine in patients with UAP was significantly higher than those of SAP patients (P < 0.05).</p><p><b>CONCLUSION</b>Upregulated monocyte chemotactic factors (MCP-1, RANTES and Fractalkine) might promote coronary plaque vulnerability in UAP patients.</p>

Effects of Yishen Capsule on sEPCR and CD62P in patients with chronic glomerulonephritis / 西安交通大学学报(医学版)

Objective To explore the effects of Yishen Capsule on the soluble endothelium protein C receptor (sEPCR) and P-selectin (CD62P) as well as the serum levels of immunoglobulin (Ig) and complement 3 (C3) in patients with chronic glomerulonephritis (CGN). Methods The changes of sEPCR and CD62P levels in plasma as well as Ig and C3 levels in serum were assessed in 40 normal subjects and 78 patients before and after treatment by double-antibody enzyme-linked immunosorbent assay, flow cytometry and immunoturbidimetry, respectively. Results Compared with those in normal group before treatment, the levels of sEPCR and CD62P in plasma as well as IgA in serum increased obviously (P<0.01), and the levels of IgG and IgM as well as C3 in serum decreased significantly in CGN patients (P<0.01). After three months' treatment with Yishen Capsule, the levels of sEPCR and CD62P in plasma and IgA in serum decreased significantly (P<0.05 or P<0.01), and the levels of IgG and IgM in plasma as well as C3 in serum increased obviously (P<0.05 or P<0.01). Conclusion The mechanism of Yishen Capsule's effect in treating patients with CGN may be correlated with decreasing sEPCR and CD62P levels in plasma as well as regulating the levels of Ig in serum and C3.

Two novel mutations of GLA gene in Chinese patients with Fabry disease / 中华心血管病杂志

<p><b>OBJECTIVE</b>To observe the disease-causing GLA gene mutations in Chinese patients with Fabry disease and the correlation between the genotype and phenotype.</p><p><b>METHODS</b>DNA from 2 Chinese patients with Fabry disease and their relatives were collected. The seven exons and nonjunctional regions of GLA gene were amplified with polymerase chain reaction and the products were sequenced. The correlation between the genotype and phenotype was analyzed.</p><p><b>RESULTS</b>Two mutations, G1168A and G1170A, located in 5' untranslated regions (5'UTR) were identified in the two probands and the two mutations were absent in normal controls. Three patients with the same genotype were found in the pedigree with G1168A mutation and there was no gene mutation carrier in the pedigree with G1170A mutation. Symptoms of the disease are less in female patients than that in male patients.</p><p><b>CONCLUSION</b>GLA gene mutation in 5'UTR may also be involved in the disease process of patients with Fabry disease and the phenotype is partly affected by gender.</p>