Chromatographical profiles of fluid extracts and tinctures obtained from Mikania glomerata Sprengel sterilized by gamma ray irradiation

Aerial parts of Mikania glomerata Sprengel, were irradiated with gamma rays in an apparatus with a Cesius-137 source in two different doses: 3.5 and 5.0 KGy. Double-blind HPLC studies on fluid extracts and tinctures prepared from the irradiated drug material were utilized to verify possible chemical changes. Extracts from the same plant (untreated) were used as standards. The results obtained showed that there was an increase in the coumarin content in the extracts obtained from irradiated plants (3.5 and 5.0 KGy) as well as a decrease in the o-coumaric acid concentration.