ABSTRACT
The aim of this work was to identify efficient vigor tests for differentiating the seed lots, forecasting seedling emergence in the field and assessing the physiological quality of Panicum maximum seeds. 12 seed lots from the cultivar Tanzania and 11 seed lots from the cultivar Massai were evaluated for water content, germination, first count and germination speed index, emergence and first emergence count of seedlings in sand, root length and shoot length, analysis of SVIS® images (seedling length, vigor and uniformity index) and seedling emergence in the field. The work was conducted in a completely randomized design for tests performed in the laboratory and in randomized blocks for tests in the field. The data were subjected to analysis of variance and the means compared by Scott Knott's test at 5% probability and statistical multivariate clustering analysis and principal components analysis. The shoot and root length tests are efficient for the evaluation of the physiological potential of P. maximum cv. Massai, while the seedling length, vigor index and growth uniformity index tests using image analysis, seedling emergence in sand and first seedling emergence count in sand are efficient in assessing the physiological potential of seeds of P. maximum cv. Tanzania, and providing information similar to that of seedling emergence in the field.
Subject(s)
Seeds , Seedlings , Panicum/physiologyABSTRACT
The incidence of fungi in seeds depends on the sanitary and climatic conditions in which the seeds were produced. So, the objective of this study was to identify the fungi present in commercial lots of Brachiaria brizantha cv. 'Marandu' and'Xaraés' seeds harvested in different areas in Brazil. Seeds from ten lots of each cultivar, produced in the Brazilian states of Mato Grosso do Sul, Mato Grosso, Goiás, Minas Gerais, São Paulo, and Bahia were evaluated as to moisture content, germination and sanity. The experiment was conducted according to a completely random design with eight replications of 50 seeds for each lot. In both cultivars, the seeds were found to be infected with Aspergillus sp., Chaetomium sp., Curvularia sp., Fusarium sp., and Helminthosporium sp; the highest incidences were those of Chaetomium sp. and Fusarium sp. Seeds of the 'Marandu' cultivar were the only ones showing the presence of Phoma sp. and Pyricularia grisea and those of the 'Xaraés' cultivar with Colletotrichum sp. and Nigrospora sp. The incidence of Fusarium sp., Aspergillus and Nigrospora sp. reduces 'Xaraés' seeds germination whereas it does not interfere in the 'Marandu' seeds germination. The presence of the fungus Pyricularia grisea is for the first time reported in tropical forage grass seeds produced in the Southeast and Northeast regions of Brazil.
A incidência de fungos em sementes depende das condições de sanidade e climáticas dos locais de procedência. Assim, o objetivo do presente estudo foi identificar os fungos presentes em lotes comerciais de sementes de Brachiaria brizantha cv. Marandú e Xaraés coletadas em diferentes áreas. Sementes de dez lotes de cada cultivar produzidas nos Estados de Mato Grosso do Sul, Mato Grosso, Goiás, Minas Gerais, São Paulo e Bahia foram avaliados quanto ao teor de água, germinação e a sanidade. Nas sementes de ambos os capins verificou-se a incidência de Aspergillus sp., Chaetomium sp., Curvularia sp., Fusarium sp. e Helminthosporium sp. com maior importância para Chaetomium sp. e Fusarium sp. As sementes de capim-marandú foram as únicas com presença de Phoma sp. e Pyricularia grisea e de capim-xaraés com Colletotrichum sp. e Nigrospora sp. A incidência de Fusarium sp., Aspergillus sp. e Nigrospora sp. reduz a germinação de sementes de capim-xaraés e a ocorrência de fungos não interfere na germinação de capim-marandú. O fungo Pyricularia grisea é relatado pela primeira vez em sementes de gramíneas forrageiras tropicais produzidas no Sudeste e Nordeste do Brasil.
Subject(s)
Aspergillus , Germination , Fusarium , PoaceaeABSTRACT
Os testes de germinação e de vigor são componentes essenciais do processo de controle de qualidade das empresas produtoras de sementes, e o teste de envelhecimento acelerado é um dos mais utilizados para avaliação do vigor de sementes. Nesse sentido, a pesquisa teve como objetivo estudar a metodologia do teste de envelhecimento acelerado, visando à identificação de diferentes níveis de vigor de lotes de sementes de quiabo (Abelmoschus esculentus (L.) Moench). Para isso, quatro lotes de sementes da cultivar 'Colhe Bem' e cinco lotes da cultivar 'Santa Cruz 47' foram submetidos às seguintes avaliações: germinação, emergência de plântulas, envelhecimento acelerado tradicional e em solução saturada com NaCl (38 e 41ºC durante 24, 48, 72 e 96 horas) e grau de umidade no início dos testes e após a incubação para o teste de envelhecimento acelerado. Os testes de envelhecimento acelerado tradicional e em solução saturada com cloreto de sódio, a 41ºC durante 96 horas, constituem em opções promissoras para detectar diferenças de vigor entre lotes de sementes de quiabo.
The vigor and germination tests are essential components of seed quality control for the seed industry. Accelerated aging is one for the most useful tests used for the evaluation of seed vigor. In this sense, this paper aimed to study the methodology of the accelerated aging test, in order to identify different levels of vigor seed lots of okra (Abelmoschus esculentus (L.) Moench). Four seed lots of the 'Colhe Bem' cultivar and five seed lots of the 'Santa Cruz 47' were submitted to the following assessments: germination, seedling emergence, accelerated aging by traditional procedures and saturated NaCl (38 and 41°C for 24, 48, 72 and 96 hours) and moisture content at the beginning of the tests and, after incubation to accelerated aging test. The accelerated aging test at 41°C/96 hours (traditional procedure and saturated NaCl) consisted efficient to detect vigor differences among seed lots of okra.