ABSTRACT
Abstract INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.
Subject(s)
Animals , Bacillus thuringiensis , Bacterial Toxins/pharmacology , Houseflies/drug effects , Insecticides/pharmacology , Larva/drug effects , Colony Count, Microbial , Pest Control, Biological/methods , Reproducibility of Results , Analysis of Variance , Microscopy, Electron, Transmission , ExotoxinsABSTRACT
: This study evaluated whether different strains of
Mortality rates were recorded after 1-mL suspensions of sporulated cells of 14 different strains of
Strains Bon 707, IGM 16-92, and Shi 3 showed the highest toxicity toward the larvae producing 70.5%, 64.5%, and 51.6% of larval mortality, respectively, which was significantly higher than that in the control group (p < 0.05). In contrast, strains NRS 1642, NRS 661, NRS 590 BL 856, NRS 342, ATCC 6457, Bon 712, and NRS 1247 showed limited or no pathogenic activity against the target larvae.
Our preliminary data indicated that