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Objective:To analyze the effects of continuous nursing intervention on patients with intestinal stoma using WeChat, China′s most popular messaging App.Methods:A total of 60 patients undergoing permanent intestinal stoma surgery, who were admitted to Dalian Third People′s Hospital from January 2018 to December 2019, were selected as the research objects. They were divided into the control group and the observation group with 30 cases each using random number table method. The control group adopted the traditional continuous nursing model while the observation group received both traditional continuous nursing model and continuous nursing interventions via WeChat. By using the Exercise of Self-Care Agency Scale (ESCA), City of Hope-Quality of Life-Ostomy Questionnaire Chinese Version (C-COH), and Ostomy Adjustment Inventory (OAI), the differences in self-care capability, quality of life, stoma adaptability, incidence of intestinal stoma complications, and patients′ satisfaction with the new nursing model in the two groups were compared before the intervention, 1 month and 3 months after the intervention, respectively.Results:Before intervention, there was no significant difference between the two groups in ESCA total score, C-COH score and OAI score ( P>0.05). After 1 month and 3 months of intervention, the observation group had the total ESCA scores of (91.50 ± 2.86) and (104.87 ± 3.45) points, which were higher than control group′s (81.37 ± 2.98) and (92.80 ± 2.91) points, respectively, and the differences between the two groups were statistically significant ( t=13.13, 14.38, both P<0.05); the observation group had the C-COH scores of (5.00 ± 1.13) and (6.37 ± 1.22) points, which were higher than the control group′s (3.90 ± 1.14) and (4.67 ± 1.04) points, respectively, and the differences between the two groups were statistically significant ( t=3.71, 5.69, both P<0.05); the observation group had the OAI scores of (48.13 ± 8.55) and (60.07 ± 7.43) points, which were higher than the control group′s (39.57 ± 7.43) and (43.47 ± 7.39) points, respectively, and the difference between the two groups was statistically significant ( t=4.07, 8.53, both P<0.05); after 3-month intervention, the observation group had a 16.7 % (5/30) incidence of stoma complications, which was lower than the control group′s 46.7% (14/30), and the difference between the two groups was statistically significant ( χ2=6.24, P<0.05); after 3-month intervention, the observation group had a score of (95.90 ± 1.66) points for the patient′s satisfaction with continuous care, which was higher than the control group′s (89.80 ± 2.44) points, and the difference between the two groups was statistically significant (t value was 11.13, P<0.05). Conclusions:Delivering the WeChat continuous nursing interventions for patients with intestinal stoma has improved the patients′ self-care capability, enhanced stoma adaptation level and quality of life, reduced the incidence of intestinal stoma complications, and improved patients′ satisfaction. Thereby, continuous nursing on WeChat is worthy of clinical application.
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How the brain perceives objects and classifies perceived objects is one of the important goals of visual cognitive neuroscience. Previous research has shown that when we see objects, the brain's ventral visual pathway recognizes and classifies them, leading to different ways of interacting with them. In this paper, we summarize the latest research progress of the ventral visual pathway related to the visual classification of objects. From the perspective of the neural representation of objects and its underlying mechanisms in the visual cortex, we summarize the current research status of the two important organizational dimensions of object animacy and real-world size, provide new insights, and point out the direction of further research.
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Brain Mapping/methods , Magnetic Resonance Imaging , Pattern Recognition, Visual , Photic Stimulation , Visual Cortex , Visual PathwaysABSTRACT
Objective To achieve the purpose of promoting movement function of the injury nerve by using the joint therapy of NT- 3- HUMSCs and SOCS3 gene silencing on SD rats'spinal cord injury. Methods (1) We used adherence method in vitro human umbilical cord-derived mesenchymal cells (HUMSC) during separation, purification and identification. (2) Then constructed NT-3 gene eukaryotic expression vector, which was transfected into its HUMSC, and constructed NT-3- HUMSC cell survival in vitro assay conditions and NT-3 expression. (3) We selected specific targets for SOCS3 screening and for sequence homology analysis. A negative control group was established. siRNA was designed and synthesized in vitro detection. (4) SD rats with spinal cord injury model were divided into two categories: (1) sham group with 10 rats; (2) T12 whole spinal cord injury model with 40 rats. The 40 rats were randomly divided into four groups with 10 rats in each group (saline treatment group,siRNA +NT-3-HUMSCs treatment group,NT-3-HUMSCs treatment group and siRNA treated group) . Motor function of the rats were evaluated respectively in 1, 2 and 3 months after the modeling was established successfully.Results(1) siRNA + NT-3-HUMSCs treatment group's BBB scores was significantly higher than NT-3-HUMSCs, SOCS3-siRNA and physiological saline groups ( P＜0.05) . (2) The grid climbing experiments showed that the neural functional recovery performed better in siRNA+the NT- 3- HUMSCs treatment group compared to the NT - 3 - HUMSCs, SOCS3 - siRNA and physiological saline groups (P＜0.05) . Conclusion The NT- 3- HUMSCs joint SOCS3 gene silencing in the treatment of SD rat spinal cord injury can improve the motor function of SD rat spinal cord injury.
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Objective To investigate the effect of joint therapy by NT-3-HUMSCs and SOCS3 gene silencing in promoting the injury nerve regeneration repair after spinal cord injury in SD rats. Methods (1) Adherence method was used to culture human umbilical cord-derived mesenchymal cells (HUMSC) in vitro for separation, purification and identification. (2) We constructed NT-3 gene eukaryotic expression vector, and used gene transfection technology into its HUMSC, and tested the survival of NT-3-HUMSC cells and NT-3 expression in cells. (3) We screened specific targets of SOCS3, made sequence homology analysis, and set a negative control, designed and synthesized siRNA and detected the function. (4) SD rats model of spinal cordinjury were established and divided into: 1. sham group 10; 2.T12 whole spinal cord injury model 40, were randomly divided into four groups, respectively; saline treatment group 10; siRNA + NT-3-HUMSCs treatment group 10; NT-3-HUMSCs treatment group 10; siRNA treated group 10. After each group above modeling success, they received respectively the neural electrophysiological monitoring for 12 weeks survival. (5) We perfused SD rats for fixation and collect samples, and observed the local glial scar degradation situation and axon regeneration, meanwhile, used biotin glucan fluorescent (BDA) anterograde tracing. The injury transplant area-host junction spinal cord tissues were collected to observe the corticospinal tract regeneration under microscope. Results (1) In siRNA + NT-3-HUMSCs treatment group, the transection syringomyelia was significantly reduced as compared with normal saline group (P ＜ 0.05). (2) BDA anterograde tracing results showed that in the siRNA + NT-3-HUMSCs treatment group, neural axon grew significantly compared with the normal saline group. (3) Neural electrophysiological testing 12 weeks after injury: in the treatment group, the incubation period P40 was shorter as compared with control group; in siRNA + NT-3-HUMSCs treatment group, the incubation period was shorter obviously than normal saline, but the amplitude increased obviously (P ＜ 0.05). Conclusion NT-3-HUMSCs joint with SOCS3 gene silencing can promote the injury nerve regeneration repair in the treatment of SD rat spinal cord injury.
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Glioma is the most common intracranial malignant tumor,with high incidence and recurrence rate,high fatality rate and the characteristics of low cure rate.Current treatment is given priority to with surgical treatment,auxiliary comprehensive therapy such as radiotherapy and chemotherapy.Hydrogen peroxide (H2O2) is a intermediate product of the cell oxygen metabolism which is a universal phenomenon in aerobic organisms.H2O2 is indispensable at various stages of tumor cell proliferation,infiltration and metastasis.Studies of the production of H2O2 and its function,the mechanism of apoptosis and the relationship between H2O2 and glioma cells can provide corresponding guidances for looking for a target gene for the treatment of gliomas.
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Objective To investigate the molecular mechanism of retinoic acid in targeted treatment of craniopharyngioma by detecting the expression of RARαand PPARβ/δin craniopharyngioma cells and analyzing the correlation between the expression and effect of retinoic acid. Methods The expression of RARα and PPARβ/δ in craniopharyngioma cells from 31 patients cultured in vitro was quantified by reverse transcription-PCR. The inhibition rates of RA on craniopharyngioma with different expression of RARα and PPARβ/δ were detected by using MTT assay, and the correlation between the expression of RARα and PPARβ/δand the effect of RA was analyzed. Results 1. The RT-PCR results showed that the expression levels of PPARβ/δand RARα mRNA were different. Craniopharyngioma cells from 31 patients in primary culture were divided into three groups according the expression levels of nuclear receptor: PPARβ/δ>RARα group, RARα>PPARβ/δ group and RARα>>PPARβ/δ group. 2.MTT results showed that the inhibition rate of RARα>>PPARβ/δgroup was significantly higher than the other groups under same drug, the differences had statistical significance ( <0.01) . Conclusions The expression of PPARβ/δ, RARα can be used to evaluate the effect of RA in treatment of craniopharyngioma. The craniopharyngioma with low-expression of PPARβ/δ is more sensitive to RA. Targeting higher RARα or targeting lower PPARβ/δ is beneficial to the treatment of craniopharyngiomas.
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Objective To explore the relationship between the expression of XRCC1 and glioma. Methods Total of 26 samples of glioma were divided into 4 groups:gradeⅠ,gradeⅡ,gradeⅢand gradeⅣ. The expression of XRCC1 in 26 Gliomas tissues were examined using SP immunohistochemical staining.Results The positive staining of XRCC1 protein was localized in nucleus of tumor cells in Glioma. There was no correlation among them. The difference of XRCC1 expression among gradeⅠ~Ⅳ was not significant ( >0.05) .Conclusion The difference of XRCC1 expression among gradeⅠ~Ⅳ was not significant. The expression of XRCC1 was closely correlated with the effect of radiotherapy.