ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and significance of transforming growth factor-β(1) (TGF-β(1)) and Ki-67 nuclear antigen in salivary gland mucoepidermoid carcinoma.</p><p><b>METHODS</b>The expression of TGF-β(1) and Ki-67 nuclear antigen in 20 cases of salivary gland pleomorphic adenoma and 45 cases of mucoepidermoid carcinoma were detected by the streptavidin-peroxidase immunohistochemical method.</p><p><b>RESULTS</b>Expression of TGF-β(1) (39/45) and Ki-67 nuclear antigen (43/45) in mucoepidermoid carcinoma was significantly higher than in pleomorphic adenoma (P < 0.05). TGF-β(1) expression was significantly higher in moderately/poorly differentiated mucoepidermoid carcinoma (16/16) than that in well differentiated mucoepidermoid carcinoma (23/29) and pleomorphic adenoma (12/20) (P < 0.05), but not different between well differentiated mucoepidermoid carcinoma (6/23) and pleomorphic adenoma (8/12) (P > 0.05). The expression of Ki-67 was increased with the increment of TGF-β(1) expression in mucoepidermoid carcinoma (P < 0.05).</p><p><b>CONCLUSIONS</b>The high expression of TGF-β(1) may play an important role in cell differentiation and malignant proliferation of mucoepidermoid carcinoma.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Adenoma, Pleomorphic , Metabolism , Pathology , Carcinoma, Mucoepidermoid , Metabolism , Pathology , Cell Differentiation , Ki-67 Antigen , Metabolism , Salivary Gland Neoplasms , Metabolism , Pathology , Transforming Growth Factor beta1 , MetabolismABSTRACT
Objective To study the effect of Fas gene transfection on bladder cancer cells. Methods Fas cDNA were transduced into bladder cancer cells EJ by DOTAP liposomal transfection regeant. Northern blot,in situ hybrization and flow cytometric evaluation were used to confirm the Fas mRNA and protein expression. The apoptosis and proliferation of EJ cells pre and posttransfection induced by cisplatin were analysed by flow cytometry、DNA ladder and MTT methods. Results Transfection of Fas gene can significantly upregulate the expression of Fas in human bladder cancer EJ cells. Apoptosis and decrease of proliferation were easily induced by cisplatin in the transfected EJ cells. Conclusions Fas system might involve in the development and progression of urogenital malignant tumors. Transfection of Fas gene by lipofectin can significantly upregulate the Fas expression in target cell. The synergistic cytotoxic effect obtainted in EJ cells suggested that combined use of Fas gene transfection and cisplatin may help in the treatment of cisplatin resistant bladder cancer.