ABSTRACT
Hepatitis B vaccination and blocking mother-to-child transmission of HBV are of great significance in the prevention and control of HBV infection. Hepatitis B vaccination is the most effective way to prevent HBV infection. Mother-tochild transmission of HBV can be greatly reduced by these measures, which include strengthening the screening of HBV in women of childbearing age, antiviral treatment in pregnant women with high viral load, and combined immunization of hepatitis B vaccine and hepatitis B immunoglobulin for newborns of HBsAg positive mothers. In the recent 30 years, remarkable achievements have been made in the prevention and control of HBV infection in China.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possibility of differentiation of human mesenchymal stem cells (hMSC) into epidemic cells in vitro.</p><p><b>METHODS</b>hMSCs were segregated from normal adult human bone marrow by Percoll solution (1.073 g/ml) , and were cultured, purified, and amplified to 3th passage in vitro. Then the hMSCs were randomly divided into control group ( with treatment of normal L-DMEM medium) and experimental group (with treatment of L-DMEM medium containing epidermal growth factor,insulin,tretinoin, calcium chloride). After 7 days of culture, the morphologic changes of hMSCs in the 2 groups were observed with inverted phase contrast microscope. The expressions of P63 and PCK of hMSCs were assessed with immunohistochemical methods.</p><p><b>RESULTS</b>The shape of hMSCs in experimental group became irregular or oblong in shape, while that in control group were still in spindle shape. Immunohistochemical results showed that hMSCs were P63 and PCK positive in the experimental group, while those in control group were negative.</p><p><b>CONCLUSION</b>Human mesenchymal stem cells can differentiate into epidemic cell in vitro.</p>
Subject(s)
Humans , Bone Marrow Cells , Cell Biology , Metabolism , Cell Differentiation , Cells, Cultured , Epithelial Cells , Cell Biology , Keratins , Metabolism , Membrane Proteins , Metabolism , Mesenchymal Stem Cells , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To isolate MSCs from adult human bone marrow cells and to induce them into adipocytes.</p><p><b>METHODS</b>MSCs were isolated from adult human bone marrow aspirated by Percoll and expanded in L-DMEM. The surface antigen of MSCs, CD14, CD34, CD45, CD44, VLA-1, HLA-DR and cell cycle were analysed on a FACScan flow cytometer. MSCs were cultured in adipogenisis inducing medium including insulin, 1-methyl-3-isobutylxanthine, indomethine and dexamethasone for 7 days and stained with Oil Red O.</p><p><b>RESULTS</b>MSCs grew as adherent cells and expanded more than 10 passages. They were positive for CD44 and negative for CD14, CD34, CD45, HLA-DR. The expression of VLA-1 was weak. After 7 days of adipocyte inducing, about 85%of the cells displayed accumulation of lipid vacuoles, as detected by Red Oil O.</p><p><b>CONCLUSION</b>MSCs isolated and cultured from adult human bone marrow can be induced to adipogenisis committed differentiation.</p>