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OBJECTIVE： To rapidly identify chemical components of Zhitong huazheng capsules （shorted for “ZTHZC”）， and to provide reference for further elucidating the pharmacodynamic material basis and overall quality control. METHODS： UPLC-Q-TOF/MS method was adopted to separate the chemical components of ZTHZC. By reviewing literatures that related to chemical components of ZTHZC and its single Chinese traditional medicinal crops， the chemical structure of the chemical components was saved into a .mol format file， and supplemented the UNIFI database. The chemical constituents of ZTHZC were qualitatively analyzed by UNIFI database screening， and validated according to their precise molecular mass， characteristic ions， neutral loss， secondary mass spectrometry cleavage， chromatographic retention behavior， combined reference information and literature reports. RESULTS： A total of 70 chemical components were identified in this experiment， including 38 organic acids and organic acid esters， 8 alkaloids， 9 flavonoids， 5 triterpenoid saponins， 5 aldehydes， 2 ketones， 1 quinone， 1 polyacetylene and 1 monoterpenoid. CONCLUSIONS： This study provides a rapid detection and identification method for chemical components of ZTHZC， and also provides a scientific basis for the clarification of ZTHZC’s quality control and pharmacodynamics.
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Objective To evaluate the uncertainty of the pseudo-ginsenoside GQ (PGQ) concentration in human plasma by HPLC-MS/MS.Methods The whole process of PGQ determination by HPLC-MS/MS in human plasma was evaluated and the uncertainty caused by repeatability,weighing,standard solution preparation,biological sample preparation,extraction recovery process,recovery,instrument precision and calibration curve fitting were evaluated,respectively.The combined and expanded uncertainty values were both calculated.Results The expanded uncertainty values for low (15.16 ng·mL-1),medium (2 516.67 ng·mL-1) and high (3 902.00 ng·mL-1) levels of PGQ were 1.39,177.74 and 262.69 ng·mL-1,respectively (P =95 ％,k =2).Conclusion The uncertainty of the PGQ determination in human plasma by HPLC-MS/MS is mainly caused by recovery,repeatabihty and sample preparation at low concentration,by sample preparation and recovery at medium and high concentration.
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Objective To observe the effect of panax quinquefolium fruit saponin(PQFS) on hemodynamics and correlative indexes in the coronary artery ligated dogs.Methods The dogs were divided into five groups randomly(n=6): control group,gingkgo leaf tablet group(24 mg?kg~(-1)),24,12 and 6 mg?kg~(-1) PQFS groups.The myocardial infarction size(MIS)and the changes of the serum enzymes were determined by using the acute myocardial infarction models with the ligation of left anterior descending coronary artery of dogs.The parameters of hemodynamics and myocardial oxygen metabolism were measured in the anesthetic dogs with thoracotomy.(Results Compared) with control group,the myocardial blood flow was increased(P
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Objective To study the ameliorative effects of pseudoginsenoside GQ (PGQ) on electrocardiogram changes in rats with acute myocardial ischemia induced by isoproterenol (ISO). Methods 50 Wistar rats were divided into five groups (n=10) randomly. Group I : i. v. 0.9% sodium chloride water liquor; Group II : i. v. verapamil hydrochloride of 0. 2 mg ? kg-1 ; Group III : i. v. 3. 0 mg ? kg-1 of PGQ; Group IV : i. v. 6. 0 mg ? kg-1 of PGQ; Group V : i. v. 12. 0 mg ? kg-1 of PGQ. Myocardial ischemia was induced by intravenous injection of ISO (2.0 mg ? kg-1). ST-segment changes in the lead II , precordial in lead V1 and precordial in lead V2 were examined. Results In lead II , lead precordial V 1 and lead precordial V 2 , ST-segment elevation of three PGQ groups were not obvious at every time point, the extents of ST-segment elevation were lower than those in sodium chloride water liquor group (P
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Objective To investigate the effects of ginsenoside Rg3 (GS-Rg3) on the hypertrophic scars of rabbit ears and provide experimental foundation for study on its inhibition on the hypertrophic scars. Methods Hypertrophic scars were proved on 24 white rabbits,of which the whole level of the skin was excised for 2 cm?2 cm,4-6 points for each ear,controlled by itself. GS-Rg3 0.1 mL(concentration 3 g?L-1) was injected into experimental group and the same volume of saline solution into control group,once every three days regionally. The scar tissues were collected 2,4 and 6 weeks after the injection respectively,the thickness of the scar,structure under the microscope,and the expressions of PCNA,Bcl-2 and Bax were observed. Results In control group,three weeks after the epithelization of the wound,the thickness of the hypertrophic tissue was 3-4 times of ventro ear skin. Under microscope,the dermis was hyperplasia and got thicker,consisted with amount of fibroblast cells,collagen and vessels,the collagen was untidy,nodule or vortex,and the cartilage could be observed in some region.In experimental group, six weeks after the injection,the skin got thinner,the collagen became neath and the quantity of the vessels decreased. In the hypertrophic scars,there was high expression of PCNA,the percent of positive cells was higher (39.55%?6.07%) compared normal tissue (11.18%?1.71%).In GS-Rg3 group,the expression of Bcl-2 was gradually decreased two weeks after injection and obviously decreased six weeks later,there was significant difference compared with before injection (P
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Four compounds were isolated from the fruit of Panax quinquefolius L. , They were iden-tified as 20 (R)-ginsenoside-Rg3( Ⅲ ), ginsenoside-Ra1( Ⅱ ), pseudo ginsenoside-RTs (iⅣ) and a new com-pound named quinquetriose ( Ⅰ ). Its structure was established as β3-D-xylopyranosyl-(1→6)-α-D-glucopy-ranosyl (1→6)-β-D-glucopyranoside, on the basis of chemical and spectroscopic evidences.
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Objective To study the influences of ginsenoside Re on learning and memory abilities of natural apolexis rats and to probe its preliminary mechanism.Method Water maze test for old rats was used to observe the effect of ginsenoside Re on learning and memory,and electrophysiological technique to record the long-term potentiation(LTP)in basic synaptic transmission of the dentate gyrus in anesthetized rats.Results Ginsenoside Re can markedly counteract memory acquisition impairment in natural apolexis rats,and enhance the synaptic transmission in the dentate gyrus and form the LTP consequence.Conclusion Ginsenoside Re can improve the learning and memory obstacle in rats,the mechanism may correlate with its enhancing the basic synaptic transmission and promoting the magnitude of LTP of the dentate gyrus.