ABSTRACT
An experimental development based on the combination of microwaves action with one of the methods of silver staining by Del RÝo Hortega is presented. Materials from normal and pathological tissues were studied. The stuff were fixed in 10 formalin and received a paraffined embedding. The impregnation was made with ammoniacal silver carbonate in a domestic microwave oven during twelve seconds at 375 w. As a result of this, a time shortage of the technique, the high degree of definition of the nuclear, cytoplasmatic and extracellular matrix (collagenus fibbers with an unsteady reticular representation) details and the easy visualization of mitosis was observed. Also, omitting the reduction, they were clearly evidenced the argirophilics granules (melanin pigment)
Subject(s)
Humans , Carcinoma, Squamous Cell/pathology , Carcinoma, Basal Cell , Silver Staining/methods , Tissue Fixation/methods , Microwaves , Skin NeoplasmsABSTRACT
Se describen los signos clínicos de paracoccidioidomicisis (PCM) y la lepra lepromatosa observados en un hombre de 58 años de edad, procedente de la zona endémica argentina de ambas patologías. El diagnóstico se confirmó por exámenes micológicos, bacteriológicos e histopatológicos y el paciente falleció sin haber podido comenzar el tratamiento. Este caso constituye la primera asociación de lepra y PCM observada por este equipo de trabajo, se destaca que en las publicaciones consultadas no se constató dicha coexistencia pese a la superposición de las endemias en Sud América