ABSTRACT
ABSTRACT Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecoma stans extracts against lung cancer cell line in comparison with vincristine drug. The antioxidant activity was studied using the standard DPPH assay and the cytotoxic activity using MTT assay. DPPH assay results show that methanolic extract of T. stans in higher concentration show better antioxidant potential than the standard L-ascorbic acid. They exhibited strong antioxidant potential at 20 µg/mL concentration. The absorbance at 517 nm showed that in the range of 0.201-0.0203 compared to that of absorbance of ascorbic acid at 0.023.Cytotoxic activity was studied using MTT assay which showed that the increase in concentration of extract increases the cell death. At 100µg/mL concentration there is an increased cytotoxic activity, i.e., 99% of cell inhibition. The results of antioxidant and anticancerous activity may be positively correlated.
Subject(s)
Plant Extracts/analysis , Cell Line , Bignoniaceae/adverse effects , Cytotoxins/analysis , Lung Neoplasms/prevention & control , Antioxidants/analysisABSTRACT
The strawberries [Fragaria x ananassa] of Rosaceae family are an accomplished source of bioactive compounds such as ascorbic acid and diverse range of polyphenols including anthocyanins, phenolic acids, flavonols, ellagitannins etc. These phenolic compounds classify strawberry as an important health promoting food. Strawberries are proved to have potent antioxidant capacity in various in vitro assay systems. The in vivo beneficial effects are getting explored against various ailments including cancer, metabolic syndrome, and cardiovascular diseases. The present research study was designed to analyze the effect of strawberry fruit extracts [water and methanol] against alloxan induced hyperglycemia in albino rats of Wister strain. Upon alloxan [150mg/kg body weight] induction, the diabetic animals showed marked increase in the values of plasma glucose, urea, uric acid, creatinine and concomitant decrease in body weight and plasma insulin level. The oral administration of strawberry extracts for 45 days in diabetic animals reversed the biochemical changes significantly [P[private use character]0.05] to near normal. Furthermore, the restoration of body weight loss was also observed. The results suggest that the strawberry extract has effective hypoglycemic activity against alloxan diabetes. The poly phenolic antioxidant contents of the strawberry fruit extracts are responsible for the observed biological effect
ABSTRACT
Fishery waste and by-products are valuable sources of raw material for recovery of antioxidant and bioactive 3tides
Due to the increased demand for protein hydrolysates with antioxidative properties by various sectors of nsumable food, health care and pharmaceutical industries, the present study focused in the production of fish protein olysate [FPH] by enzymatic digestion from the backbone of Rastrelliger kanagurta [Indian mackerel] and evaluated | antioxidant potential
The observed results of the degree of hydrolysis suggest that the rapid phase of proteolytic savage was occurred in the first 60 minutes of incubation and during this period, the rate of hydrolysis was found to be with increasing ratio of enzyme to substrate concentration
The result of the antioxidant properties clearly tficates that thel, 1- diphenyl-2 picrylhydrazyl [DPPH] radical scavenging efficacy of FPH was similar to that of Jtic antioxidants like butylated hydroxyl toluene [BHT]
The FPH also exhibited significant reducing power ability I great potential to inhibit lipid peroxidation in equivalence with that of synthetic and natural antioxidants such as fand a-tocopherol respectively
The overall findings of the study reveal that, FPH produced by tryptic digestion has able amount of bioactive peptides with potent antioxidant properties
The synthesized FPH is a good candidate irfurther development into a commercial food additive