ABSTRACT
Blood group antigens include more than 260 antigens on the erythrocyte cell membrane,forming 24 different blood group systems. These can be categorized in 11 major systemswhich account for 172 of the antigens. Incompatibility of some of these antigens, especially inmultiple transfused patients, results in hemolytic reactions. Although intermediate thalassemicsneed fewer blood transfusions than major ones, alloimmunization is more frequent in the former. This can be due to the different frequenct rates of blood group phenotypes in intermediate thalassemics as compared with healthy people.Red blood cell antigen typing was done in 39 intermediate thalassemics and 150 healthy blood donors for Rh, Kidd, MNSs, Lutheran and Duffy blood systems. K and M antigens had different frequency rates in both groups [p<0.01, p<0.0005] showing higher frequency inpatients.Complementary studies are recommended to be performed in order to evaluate high-riskalloimmunization in intermediate thalassemias. Assessment of the frequency of other red bloodcell antigens, antibody screening, and identification of alloantibodies in immunizedintermediate thalassemics can be helpful in this regard.Kantigen is very immunogenic; however its prevalence rafe is high among intermediatethalassaemics, but this contradiction can not be posed as the increasing cause of alloimmune reactions in such patients. Supplemental studies including screening and detection of posttransfusioncreated antibody types not only help in selecting the more appropriate blood typefor thalassaemics but also help in justifying increasing alloimmune reactions in thalassaemics
ABSTRACT
In this study our aim was to determine HLA-Class I and II antigens freguencies of Hamedani ethnic group. In addition to demographic studies and disease association, it has wide application in bone marrow donor registeries. In order to establish DNA-based HLA typing in central Laboratory of Iranian Blood Transfusion Organization, a comparison of serological and molecular [sequence specific primers "SSP"] methods for HLA-DRB has been performed. The study was descriptive and the population under study were selected out of the native people of Hamedan; 100 healthy volunteer blood donors were chosen by questionnaire. 10ml heparinized and 3ml EDTA blood were collected from each selected donor. EDTA [PCR] samples were then frozen.N.I.H standard microlymphocytotoxicity and Nylon wool T and B cells separation was used for serological I and II typing. HLA-Class I plates were prepared from Iranian Blood Fractionation and Research Company and for Class II we used Biotest DR/DQ Typing Trays. PCR was done using "Roche high pure DNA extraction" Kit and HLA-DRBSSP [Biotest]. The most and least frequent HLA-B antigens were B5 group [B51/B52] and B16 [38,39] respectively. Because of low resolution of HLA-DRB Kit, no significant difference was observed between serological and PCR methods. Although some blanks have been determined by PCR.The HLA-DRB determination by PCR is mandatory for donor/recipient pairs [even sibling] for bone marrow transplantation; for donors it should be done by high resolution kits