ABSTRACT
The aim of this study was to evaluate in vitro anti-proliferative [tested on MCF-7, MDA-MB-231, and MCF- 10A cell lines] and anti-inflammatory [evaluated as inhibition of prostaglandin E[2] synthesis catalyzed by cyclooxygenase-2] effect of various extracts from Vaccinium bracteatum leaves and fruits. The highest anti-proliferative effect possessed leaf dichloromethane extract with IC[50] values ranging from 93 to 198 microg/mL. In the case of cyclooxygenase-2 inhibition, n-hexane, dichloromethane, and ethanol fruit extracts showed the best activity with IC[50] values = 2.0, 5.4, and 12.7 microg/mL, respectively. These results indicate that V. bracteatum leaves and fruits could be useful source of anti-cancer and anti-inflammatory compounds.
Subject(s)
Humans , Anti-Inflammatory Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Plant Extracts/pharmacology , Plant Leaves , Cell Line, Tumor , Cell Proliferation/drug effects , FruitABSTRACT
Crude methanolic extracts of selected legumes namely, black gram [Vigna mungo L.], green gram [Vigna radiata [L.] R. Wilczek], soybean [Glycine max [L.] Merr.] and lentil [Lens culinaris Medik.] were investigated for anti-inflammatory effects, using COX-2 producing PGE[2] inhibitory assay. Percentage inhibition observed was 73.93, 79.84, 92.17 and 74.47 for black gram, green gram, soybean and lentil respectively at 20microg/ml extract concentration. The l00 micro g/ml concentration showed increase in the percent inhibition except for soybean. This is the first report on COX-2 inhibitory potential of food legumes