Analysis of motivating factors for eye donation among families of eye donors in South India – A questionnaire-based study

Purpose: To report the factors influencing eye donation among families of successful eye donors in India. Methods: The consenting family members of 434 deceased individuals who donated eyes between April 2019 and March 2020 were retrospectively interviewed via a telephonic questionnaire survey. Details regarding the donors and their families, motivating factors for eye donation, and time taken for tissue recovery were collected and analyzed. Results: The mean age of donors was 66.8 years, and only 13.9% of them had pledged to donate their eyes before death. For 62.3% of donations, children of donors were the primary consenters for eye donation. In 18.8% of donors, there was a previous history of eye donation in the family. Many donations were motivated by a non?governmental organization volunteer (40.5%) or by a grief counselor at the hospital (27.4%). Hospital?based corneal retrieval programs and donations where the first eye bank contact was made through hospital personnel had the greatest percentage of rapid enucleations (performed within 3 h after death) (48.7% and 49.1%, respectively; P = 0.001 and P = 0.02, respectively). Conclusion: Children of donors, typically in the working?age group, most often make the decision for donation, and hence, future awareness programs should focus on this specific population. All types of hospitals should advocate for eye donation as they are a common point of contact for a grieving family, and health?care professionals at all levels should be considered for training as motivators for eye donation.

Synthesis and electrochemical properties of environmental free L-glutathione grafted graphene oxide/ZnO nanocomposite for highly selective piroxicam sensing / 药物分析学报

A simple and reliable strategy was proposed to engineer the glutathione grafted graphene oxide/ZnO nanocomposite(glutathione-GO/ZnO)as electrode material for the high-performance piroxicam sensor.The prepared glutathione-GO/ZnO nanocomposite was well characterized by X-ray diffraction(XRD),Fourier transform infrared spectrum(FTIR),X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscopy(FE-SEM),cyclic voltammetry(CV),electrochemical impedance spectros-copy(EIS)and differential pulse voltammetry(DPV).The novel nanocomposite modified electrode showed the highest electrocatalytic activity towards piroxicam(oxidation potential is 0.52 V).Under controlled experimental parameters,the proposed sensor exhibited good linear responses to piroxicam concentrations ranging from 0.1 to 500 μM.The detection limit and sensitivity were calculated as 1.8 nM and 0.2 μA/μM·cm2,respectively.Moreover,it offered excellent selectivity,reproducibility,and long-term stability and can effectively ignore the interfering candidates commonly existing in the pharmaceutical tablets and human fluids even at a higher concentration.Finally,the reported sensor was successfully employed to the direct determination of piroxicam in practical samples.

Perioperative Hypersensitive Reaction to Hyaluronidase.

Hypersensitive reactions are one of the problems faced by Anaesthesist.In this case report we present a case who developed hypersensitive reaction following a peribulbar block with hyaluronidase.In this study we stress to perform test dose for hyaluronidose especially if non recombinant preparations are used.

Effects of alcohol on micronucleus in human exfoliated buccal cells.

Background: Oral cancer is the most common malignancy in nearly half of Indian population. The main causes of oral carcinoma are tobacco, alcohol, poor diet and infective agents. These agents damage the chromosomes to form several secondary nuclei known as micronuclei. This study identifies the occurrence of micronuclei and also evaluates the frequency of micronuclei in stained smears of oral exfoliative cells from healthy subjects and alcoholic subjectsMaterials and methods: A total number of 60 alcoholic subjects were referred to the Department of Anatomy, SRM Medical College and Research Center, SRM Nagar for micronucleus assay from the Department of Dentistry. Equal numbers of controls were included with normal looking oral cavities. Results: Out of 60 alcoholic subjects 43 showed presence of micronuclei and out of 60 control subjects, only 6 showed micronuclei. With these observations, alcohol is one of the predisposing factors of oral carcinoma.Conclusion: It is evident from our present study, it is clear that in alcohol consumption, the buccal mucosa, which are at high risk for development of oral cancer, show an increase in the MN frequencies.

A Computerized Tomographic Study of Uncinate Process of Ethmoid Bone.

Background: The uncinate process is an important landmark in the anatomy of osteo-meatal complex of frontal recess which also plays a vital role in the ventilation of middle meatus and sinuses. Its superior attachment shows great anatomic variability. The aim of this study was to observe and classify superior attachment and presence of pneumatisation in uncinate process. Materials and methods: Computed tomographic images of paranasal region from 100 patients were studied retrospectively. In 100 patients, 54 belonged tomale and 46 female and were in the age group of 11 to 75 years with an average of 32.7 years. The superior attachment of uncinate process was observed and tabulated according to Landsberg and Friedman classification and pneumatisation of uncinate process was also noted. The results were analysed statistically. Results: The superior attachment of uncinate process was observed in 200 sides out of 100 patients and its attachment to the agger nasi cells (type - 2) was found in 36% while its attachment to lamina papyracea (type – 1) and to middle turbinate (type – 6) were found in 19% and 20% respectively. Uncinate process ending at the junction of middle turbinate with cribriform plate (type – 4), at the ethmoid skull base (type – 5), bifurcating towards lamina papyracea and junction of middle turbinate with cribriform plate (type – 3) were seen in 2%, 8% and 5% respectively. In 11%, the superior end showed no attachment to surrounding structures. The uncinate process was pneumatised in 34 of 200 sides (17%), among which 45.5% was unilateral and 54.5% bilateral. Conclusion: Preoperatively evaluating variations of uncinate process and its pneumatisation helps to avoid intraoperative damage to surrounding structures. The detailed knowledge of extent of uncinate process may also help to deduce the reason for refractory chronic sinusitis.

Anomalous Unfused Pancreatic Duct Pattern.

Pancreas divisum is a congenital anomaly of the duct system of pancreas that occurs due to the failure of fusion of dorsal and ventral pancreatic ducts. This anomaly has been hypothesized as the predisposing factor for chronic, recurrent and idiopathic pancreatitis. During routine dissection, we observed 2 cases of complete type of pancreas divisum. In the observed cases, the patent dorsal pancreatic ducts terminated onto minor duodenal papilla and measured 15.6 cm and 17.3 cm respectively. The patent ventral pancreatic ducts measured 3.2 cm and 2.7 cm respectively and terminated onto the major duodenal papilla after joining with common bile duct. The major papillae were antero-superiorly related to minor papillae and the distance between them measured 4.3cm and 1.4 cm respectively. Awareness and timely detection of this commonly occurring pancreatic anatomical anomaly helps the clinicians to prevent or manage potential recurrent pancreatitis.

Infant with Type A Niemann Pick Disease and Undetectable Niemann Pick Cells in Bone Marrow.

Bone marrow aspiration is the preliminary investigation in Niemann Pick disease type A when enzyme assays and mutation studies are unavailable. We report an infant with typical phenotype and enzyme deficiency, but undetectable Niemann Pick cells in the bone marrow. A new mutation R542X in SMPD gene was also detected.

Polymerase chain reaction confirmation of diagnosis of intestinal amebiasis in Puducherry.

Background Stool microscopy, the commonly used test for diagnosis of intestinal amebiasis, is unreliable as it does not differentiate Entamoeba histolytica, the causative agent for amebiasis, from non-pathogenic Entamoeba dispar and Entamoeba moshkovskii. Methods Two hundred and forty-six stool samples collected between January to February 2009, were examined microscopically for E. histolytica/E. dispar/E. moshkovskii complex cysts and trophozoites. Nested multiplex PCR targeting a 16 S-like rRNA gene for differential detection of all the three morphologically similar forms of E. histolytica, E. moshkovskii and E. dispar simultaneously was done on all microscopy positive stool samples. Results Forty-nine stool samples were positive for Entamoeba spp. by microscopy. Nested multiplex PCR for E. histolytica, E. moshkovskii and E. dispar was positive in only 19 of these samples, and only 6 of the 19 samples were positive for E. histolytica. Conclusion Stool microscopy grossly over-diagnosed intestinal amebiasis and is likely to result in unnecessary treatment with anti-protozoal drugs.