ABSTRACT
N-[2-Hydroxybenzyl]-2-amino-2-deoxy-D-glucose [NHADG] was synthesized by conjugation of salicylaldehyde to glucosamine. The obtained compound was well characterized via different analytical techniques. Labeling of the synthesized compound with technetium-99m [[99m]Tc] in pertechnetate form [[99m]TcO4[-]] was carried out via chelation reaction in the presence of stannous chloride dihydrate. Maximum radiochemical yield of [99m]Tc-NHADG complex [99%] was obtained by using 1 mg NHADG, 200 micro g SnCl[2].2H[2]O, at pH 9.5 and reaction time of 15 min. The radiochemical purity of the [99m]Tc-NHADG complex was measured by Instant Thin Layer Chromatography [ITLC] and Paper Chromatography [PC], without any notable decomposition at room temperature over a period of 4h. The biological evaluation results show that the [99m]Tc labeled NHADG conjugate is able to specifically target mammary carcinoma in mice models, thus highlighting its potential as an effective [99m]Tc labeled glucose-derived agent for tumor imaging