Clinical values of using platelet rich plasma with autologous fat injection in facial rejuvenation / 中华医学美学美容杂志

Objective To explore the clinical value of autologous fat injection with platelet rich plasma in facial rejuvenation.Methods 35 female beauty seekers were collected in this study.They were divided into two groups:sample autologous fat group (FAT group,n=20) and combined autologous fat and platelet-rich plasma group (PRP group,n =15).The average level of injection was 34 ml.The fat was collected at the abdominal or thigh sites using 18 G needle connecting with negative pressure suction-pump.The fat was centrifugated 2 min to get rid of the lower layer of water and the upper layer of oil.Weigao PRP extract kit was used to obtain the PRP.The ratio of PRP to fat was 1:5.The injection technique was according to Colemen method.The survival rate,satisfaction and complication were compared between the 2 groups.Results There were no infection,hematoma,fat liquefaction necrosis occurred in the 2 groups.Compared with the FAT group,the skin texture and facial contour of the PRP group was improved.Conclusions The platelet rich plasma can improve the survival rate of fat and has a stable effect,which is one of the safe and ideal methods of facial rejuvenation.

Beneficial effects of silver foam dressing on healing of wounds with ulcers and infection control of burn patients

Objective: To assess the beneficial effects of silver foam dressing on the healing of wounds with ulcers and infection control of burn patients

Methods: Eighty-four second-degree burn patients were selected and divided into a study group and a control group [n=42]. After disinfection and cleaning, wound beds of the study group were covered with silver-containing soft-silicone foam dressing, and wound surfaces of the control group were wiped with 1% silver sulfadiazine cream [60 g/100 cm2]. The two groups were checked weekly to observe wound healing progress and adverse reactions of the skin around wounds. Wound secretions were collected and subjected to bacterial culture. Related indices were recorded and quantified

Results: Thirty seven cases of the study group [88.1%] and 36 cases of the control group [85.7%] recovered to normal, and 3 [7.1%] and 2 cases [4.8%] in the two groups failed to recover. The recovery rates of the two groups were similar [P>0.05], but unrecovered patients in the study group had significantly higher proportions of repaired wounds [P<0.05]. Wounds of the study group were healed significantly more rapidly than those of the control group [22.3+/-3.1 vs. 25.1+/-4.4, P<0.05]. The study group had significantly higher proportions of repaired wounds from Day 7 to Day 21 [P<0.05], but the difference became less obvious with extended time to Day 28. The bacterial culture-positive [exceeding 10[5] organisms per gram of tissue] rates of both groups significantly reduced after treatment [Day 7 for the study group and Day 14 for the control group], and the rate of the study group was significantly lower at last [P<0.05]. The study and control groups were observed 134 and 149 person-times respectively, with the normal wound-surrounding skin rates of 96.3% [129/134] and 88.6% [132/149] [P>0.05 except for on Day 14]. Except for on Day 28, the study group had significantly lower pain scores than those of the control group [P<0.05], especially on Day 7 and Day 14 [P<0.01]. From Day 7 to Day 28, the study group was significantly less prone to burning sensation than the control group [P<0.05], but both groups felt anxious during dressing change [P>0.05]. Dressing of the study group was changed significantly more easily [P<0.05], but the fixing outcomes were similar [P>0.05]

Conclusion: Silver foam dressing rapidly, easily and safely resisted wound bacteria, promoted wound healing and shortened recovery time, effectively relieving the pain of patients

Clinical efficacy of dual plane breast augmentation using perinipple incision / 中华医学美学美容杂志

Objective To study the technology and the result of dual plane breast augmentation using nipple margin vertical incision of areola.Methods Totally 60 cases of augmentation mammaplasty were involved in this study.The nipple margin vertical incision of areola was applied obliquely into the breast through the pectoralis major fascia.The rib starting point of pectoralis major were cut off,medial to the side of the sternum.Under the pectoralis major the cavity was peeled according to the preoperative design range.Based on the different situation of the breast types Ⅰ,Ⅱ,Ⅲ,dual plane breast augmentations were stripped respectively.After implanting the breast prosthesis,the upper part of the prosthesis was under the pectoralis major and the lower part was under the mammary gland.Results The 60 patients were all after childbearing,20 of whom underwent type 2 dual plane breast augmentation,4 underwent type 3 double plane and the rest underwent type 1 double plane.After 3 months to 2 years follow-up,all cases got satisfactory results,except 1 case of postoperative hematoma and 1 case appeared capsular contracture.Conclusions The nipple margin vertical incision of areola can complete types Ⅰ,Ⅱ,Ⅲ dual plane breast augmentation operation,at the same time it can correct mild-to-moderate mastoptosis.

Preliminary study of the role of canonical Wnt signalling pathway in non-Hodgkin's lymphoma cell lines / 肿瘤

Objective: To determine whether the canonical Wnt signalling pathway is abnormally activated in NHL (non-Hodgkin's lymphoma), and to explore its association with pathogenesis of NHL. Methods: The expression levels of β-catenin, GSK-3β (glycogen synthase kinase-3β) and its inactive form p-GSK-3β (Ser9) (phosphorylation of GSK-3β at Ser9 residue) in NHL cell lines including SUDHL-4, Raji and Namalwa were detected by Western blotting, and the normal human lymphocytes were served as the controls. The mRNA levels of CTNNB 1 gene (encoding β-catenin), canonical Wnt signaling pathwayrelevant gene LRP 5 (low-density lipoprotein receptor-related protein 5) and target gene c -Myc were detected by real-time fluorogenic quantitative PCR. Results: The expressions of total cellular proteins and β-catenin protein in cell nuclei were significantly up-regulated in three NHL cell lines as compared with in those normal human lymphocytes. The elevated expression levels of p-GSK-3β (Ser9) were observed in lymphoma cell lines, but the expression level of GSK-3β was not significantly different between the lymphoma cell lines and the normal human lymphocytes. The mRNA expression levels of CTNNB1, LRP5 and c-Myc were significantly higher in lymphoma cell lines than those in the normal human lymphocytes. Conclusion: Given the important role of β-catenin as an activation marker in canonical Wnt signaling pathway, the evidence of up-regulated expression of β-catenin and the changes of other relevant molecules in canonical Wnt signaling pathway suggests that the constitutional activation of canonical Wnt signaling pathway may contribute to the pathogenesis of NHL. Copyright © 2013 by TUMOR.

Advances in research on canonical Wnt signaling pathways in malignant lymphomas / 肿瘤

Wnt signaling pathway is an important pathway for regulating the development of embryo and organs. It has been shown to play a key role in proliferation, differentiation, polarity, adhesion and motility of cells. The constitutive activation of canonical Wnt pathway is involved in the pathogenesis of many carcinomas. In recent years, accumulating studies have suggested its association with the pathogenesis of malignant lymphomas. This review summarizes the latest progress in research on canonical Wnt pathway and its role in lymphocyte development and the occurrence of malignant lymphomas, in order to provide the new clues of mechanism for pathogenesis of lymphomas and then disclose new potential therapeutic targets which are based on this pathway. Copyright © 2012 by TUMOR.

Clinical application of intraoperative sustained skin and soft-tissue expansion in augmentation mammaplasty using transaxillary approach with endoscopic assistance / 中华医学美学美容杂志

Objective To evaluate the results of prosthesis augmentation,relieve suffering patient experience,improve shape and touch of breast.Methods According to design,in dual-plane mammaplasty transaxillary approach with endoscopic assistance,we used soft-tissue expansion before prosthesis,saline volume in expander was the same as prosthesis size at beginning; adjusting expander volume and position,then determined prosthesis size according to expander volume lastly.after intraoperative sustained expansion for 30 to 60 minutes twice prosthes volume,replace expander with prosthesis.Results This method was applied in 120 patients,showing that pain significantly reduced after expansive without use of analgesia pump; complications included that 3 patients appeared seroma and absorbed soon; 2 patients had poor prosthese location,which was adjusted in reoperation; one capsular contracture was repaired later.After 6-months to 2-years follow-up,the results showed that breast was nature and dynamic; satisfactory rate reached to 95 ％.Conclusions In augmentation mammaplastic operation,pre-adoption of an expander can make it easy to adjust the location of prosthesis,to avoid being injured and to determine prosthesis volume by expander injection of saline volume,especially for patient with different size of both breasts.