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@#AIM: To investigate the effects of Aflibercept on the K+ channel of retinal Müller cell membrane cultured <i>in vitro</i>.<p>METHODS: Human Müller cells were divided into 3 groups(control group, high glucose group and experimental group). The control group were cultured in conventional DMEM medium; the high glucose group were cultured in high glucose DMEM medium; the experimental group were cultured with high glucose DMEM medium and 100μmol/L Aflibercept, and the K+ concentration of the cells were detected by MQAE, and the cell survival were detected by MTT assay, the flow cytometry were used to detect apoptosis rates, Western blot analysis were used to detect the Müller cell caspase-3 protein levels.<p>RESULTS: The Müller cells were positive for glutamine synthetase(GS)after 48h of culture, and the purification degree were above 90%. The relative concentrations of K+ in the control group, high glucose group and experimental group were(2.14±0.44)%,(23.11±4.39)%,(5.20±0.92)%, and cell viability were(100.00±0.00)%, respectively(73.24±4.13)%,(85.22±5.33)%, the apoptosis rates were(5.03±1.91)%,(26.73±3.14)%,(16.63±2.73)%, and compared the differences between two groups were statistically significant(<i>P</i><0.05). Compared with the control group, the level of caspase-3 protein in the high glucose group Müller cells were increased significantly(<i>P</i><0.05); compared with the high glucose group Müller cells, the caspase-3 protein level in the experimental group Müller cells were decreased significantly(<i>P</i><0.05).<p>CONCLUSION: Aflibercept can inhibit the K+ channel of retinal Müller cells <i>in vitro</i>, inhibit the apoptosis of Müller cells induced by high glucose, decrease the expression of caspase-3 protein, and promote cell proliferation.
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@#AIM: To investigate the effect of aflibercept on the expression of signal transducers and activators of transcription 3(STAT3)in rat retinal Müller cells. <p>METHODS: The rat retinal Müller cells treated with different concentrations of Aflibercept 100 μL(diluted concentrations of 400, 200, 100 pg/mL, respectively). MTT assay were used to detect cell proliferation, flow cytometry. Apoptosis were detected by the instrument, cell invasion were detected by transwell chamber method, and protein(AKT, STAT3, GAPDH)expression were detected by Western-blot method.<p>RESULTS: The proliferation activity of Müller cells were decreased with the increased of aflibercept concentration, and compared the difference were statistically significant(<i>P</i><0.05). After treatment for 48h, the apoptotic rate of Müller cells were gradually increased with the increased of aflibercept concentration, and the invasion and penetration index of Müller cells gradually were decreased, and compared the difference were statistically significant(<i>P</i><0.05). After 48 h of transfection, the relative expression of AKT protein in Müller cells were not change significantly with the increased of Aflibercept concentration(<i>P</i>>0.05), and the relative expression of STAT3 protein decreased gradually, and compared the difference were statistically significant(<i>P</i><0.05).<p>CONCLUSION: Aflibercept can inhibit the expression of STAT3 protein in rat retinal Müller cells, thereby inhibit cell proliferation and invasion and promote apoptosis.
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@#AIM: To analyze the influence factors of high intraocular pressure after laser epithelial keratomileusis(LASEK), and to discuss the clinical treatment and prognosis of the high intraocular pressure patients. <p>METHODS: A retrospective study was used. From August 2013 to February 2016, 160 LASEK patients(160 eyes)in our hospital were selected. Visual acuity after operation was recorded. The incidence and influence factors of high intraocular pressure were investigated. The treatment method and prognosis of high intraocular pressure were recorded. <p>RESULTS: In the 160 patients, all patients had complete epithelial flap and different degree of photophobia. There were 19 patients with postoperative photophobia, conjunctival hyperemia and foreign body sensation significantly. At postoperative 3mo, average intraocular pressure were 18.40±4.98mmHg, which was higher than that of preoperative, 16.27±2.24mmHg(<i>P</i><0.05), and at postoperative 3mo, visual acuity was significantly higher than preoperative(<i>P</i><0.05). There were 9 patients with postoperative steroid induced ocular hypertension, the incidence rate was 5.6%; Multiple linear regression analysis showed that the cutting depth(<i>OR</i>=3.209), maximum diameter of curvature(<i>OR</i>=3.071)and fundus C/D(<i>OR</i>=9.224)value were the main risk factors leading to postoperative steroid induced ocular hypertension(<i>P</i><0.05). <p>CONCLUSION: The postoperative steroid induced ocular hypertension in LASEK is common, but has no obvious effect on visual acuity. The cutting depth, maximum diameter, curvature fundus C/D value were the main influencing factors, so we should careful use the hormone drugs in treatment and prevention. Periodic review and detection for change of intraocular pressure are needed.
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AlM: To observe the clinical results of pterygium excision combined with conjunctival autograft transplantation using fibrin glue.METHODS:A total of 60 patients (60 eyes) with primary nasal pterygium were randomly divided into two groups:the fibrin glue group ( experimental group, 30 eyes) and suture group ( control group, 30 eyes ) . All patients underwent pterygium excision combined with conjunctival autograft transplantation. ln the experimental group autograft was attached to sclera with fibrin glue while in control group 10-0 polyamide was used. The patients were followed up for 6mo. The time of operation, post operation comfort, complications and recurrence were evaluated.RESULTS:The average surgical time was 24. 5 ± 6. 5min with fibrin glue group while 35. 2 ± 5. 4min with suture group, with statistically significant difference between two groups (P<0. 05). Pain and foreign body sensation was significantly less with fibrin glue group than that in the control group ( P < 0. 05 ). No severe postoperative complication occurred both in two groups. The incidence of subconjunctival hemorrhage in the experimental group was lower than that in the control group (P<0. 05). 1 case (3%) of fibrin glue group, 3 cases (10%) of suture group had recurrence at the end of follow up 6mo.CONCLUSlON:lt's a safe and effective way to attach conjunctival autograft during pterygium surgery by fibrin glue. lt can reduce surgical time, postoperative complications and relieve postoperative discomfort.