ABSTRACT
Objective:A questionnaire for the compliance with moxibustion was designed based on the 4-item Morisky scale, and the reliability and validity of the questionnaire was investigated.Methods:A modified Morisky scale was designed based on the 4-item Morisky scale for the compliance with moxibustion, and 146 patients having received moxibustion for over 2 weeks were investigated using this scale to evaluate their compliance with moxibustion; the internal equity and the construct validity of the scale were statistically analyzed.Results:The analysis of reliability showed that in item of internal consistency, the Cronbach's α was 0.72 and the split-half coefficient was 0.71, the correlation coefficients between the 4 component scores and the total score ranged 0.67-0.80, and the between-component correlation coefficients ranged 0.24-0.56; the exploratory factor analysis (EFA) totally extracted 1 common factor, and the explicable variation was 55.02%, and the loads of the 4 items were respectively 0.82, 0.81, 0.74 and 0.58.Conclusion:The reliability and validity of the modified Morisky scale for the compliance with moxibustion are acceptable, while several items need further modification and improvement in the expression and content.
ABSTRACT
Apoptosis refers to the programmed death process of cells modulated by genes. It plays an important role in promoting the evolution of organisms, regulating the development of multiple systems, and maintaining the stability of internal environment. The major apoptosis pathways include mitochondrial pathways, death receptor pathways, and endoplasmic reticulum pathways. With the rapid development of the scientific research on acupuncture, it’s revealed that cell apoptosis should be closely related to the action of acupuncture. So far, the major apoptosis factors involved in relevant researches are P53, Bcl-2 family, Cyt-C, Caspase, Fas/Fasl, TNFR1/TNF-?, and FADD-Caspase8, and PERK, etc. Via influencing these apoptosis factors or genes, acupuncture can produce various effects in improving tissue ischemia, protecting neural cells, reducing inflammation, and correcting endocrine disorders. This article summarized and reviewed the apoptosis pathways and key factors related to the action of acupuncture, to provide some beneficial references for future studies.
ABSTRACT
Objective] Metabolomics is a genomics research methods, has good prospects in the study of mechanisms of acupuncture. In this paper, the progress of the research on the mechanism of the action of metabolism in the mechanism of acupuncture and moxibustion were reviewed. [Methods] Retrieve acupuncture mechanism studies,which use metabolic technology in nearly 15 years, and to summarize. [Results]Nearly 15 years metabolomics research techniques and tools developed rapidly. But the acupuncture metabomoic studies were still based on the research of animal experiments mainly, relative clinical research less. [Conclusion] With the deepening development of metabolomics studies, the metabolic characteristics of various diseases will gradually clear. Acupuncture clinical mechanisms research combined with metabolomics technology will have broader prospects. But the research strategy of metabololic technology on acupuncture clinical efficacy is yet to be further explored and improved.
ABSTRACT
The development of TCM acupuncture represents a internationalized and modern trend. A recent study with the title of "Acupuncture for chronic knee pain: a randomized clinical trial" published in Journal of the American Medical Association on October 1st, 2014, which raised doubts on acupuncture efficacy as well as traditional manipulation and acupoint theory, makes some negative impact and challenges on the development of acupuncture. From the view of future development of acupuncture, the potential influence of this research on acupuncture development is proposed, and by combining acupuncture theory, some discussions and doubts on the research design and outcome explanations are made. Additionally, enlightenments of this research on further clinical research are summarized.
Subject(s)
Female , Humans , Male , Acupuncture Therapy , Chronic Pain , Therapeutics , Low-Level Light TherapyABSTRACT
This paper reviews metabolomics/metabonomics technologies used in digestive disease clinical diagnosis, summarizes mainly two aspects for using metabolomic methods in current diagnosis for gastrointestinal disease, i.e. the one used for diagnosis of gastrointestinal malignancy, the other improving diagnosis of disease which can not be easily distinguished by traditional clinical methods. The future use of the metabonomics in clinic is also analyzed in the present paper.
Subject(s)
Humans , Gastrointestinal Diseases , Diagnosis , Liver Function Tests , Methods , Metabolome , Physiology , Metabolomics , Methods , Stomach Neoplasms , DiagnosisABSTRACT
This study was carried out to defect the effects of acupuncture and moxibustion therapy on the proliferation and differentiation of neural stem cells in the olfactory bulb of senescence-accelerated mouse P8. Immunofluorescence double staining methods(BrdU/Nestin, BrdU/MAP-2 and BrdU/beta-tubulin III) were used. The results show that both acupuncture and moxibustion could improve proliferation of neural stem cells, but only moxibustion could induce differentiation of neural stem cells into neuron.
Subject(s)
Animals , Male , Mice , Acupuncture Therapy , Cell Differentiation , Cell Proliferation , Cells, Cultured , Moxibustion , Neural Stem Cells , Cell Biology , Olfactory Bulb , Cell BiologyABSTRACT
Metabonomic approaches are believed to be one of the powerful methods for disease diagnosis and assessment of patients' conditions. However, many publications have been providing the evidences of the biases caused by many factors such as diet, physical exercises, environment and individual differences in the clinical researches. For the purpose of ensuring the comparability of metabonomic 1H NMR data from different studies, it is necessary to control those nonexperimental factors in the study design and the data analysis.
Subject(s)
Humans , Data Interpretation, Statistical , Magnetic Resonance Spectroscopy , Methods , Metabolomics , Methods , Research Design , Reference StandardsABSTRACT
In term of innovating in teaching,the text proposes some rectification and improvement measures according to the weak point existing in the chemical experiment in the past.We have improved the teaching quality of the chemical experiment through improving teacher's innovative quality,adjusting the experiment content rationally,changing the teaching mode of the experiment and examining method,and optimizing the experiment means,etc.
ABSTRACT
To investigate the growth and osteogenesis characteristics of cultured canine mesenchymal stem cells (cMSCs) under osteogenic induction. We found the cMSCs were isolated from adult canine using density gradient separation method. The cMSCs attachment formed soon after seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic induction compound of Dexamethasone (Dex), beta-sodium glycerphosphate (beta-GP), ascorbic acid (AA) was added to passaged cMSCs and the proliferation and osteogenic differentiation of them was studied. The morphology of cells was observed by light micrograph and transmission electron microscope. The proliferation and growth characteristics of cMSCs were observed during primary and passage cultures through MTT. The differentiation were assayed by alkaline phophatase (ALP) and osteocalcin (OCN). We found the cMSCs have an active proliferative ability in primary and passage culture, and cMSCs under osteogenic induction have the typical characteristic of a secretory cell; the osteogenic induction compound may induce cMSCs to differentiate to osteoblasts. There are higher expression of ALP and OCN in passage 3 cMSCs under osteogenic induction than that of the osteoblasts osteogenic induction condition. Our research suggest the cMSCs in our culture system are mainly undifferentiated osteoprogenitors and can differentiate to osteoblast under osteogenic induction.
Subject(s)
Animals , Dogs , Bone Marrow Cells , Cell Biology , Cell Differentiation , Physiology , Cells, Cultured , Mesenchymal Stem Cells , Cell Biology , Osteoblasts , Cell Biology , Osteogenesis , Tissue Engineering , MethodsABSTRACT
[Objective]To study the inhibitory effect of the extract of Rhizoma Amorphophallus,we set up the Kunming mices which were vaccinated with H22 tumor as experimental model,and explore the possibility mechanism of them.[Method]We adopted the mices vaccinated with H22 tumor as experimental model to observe the influence of different extracts on the antitumor ratio,and the cooperating antitumor effect together with 5-Fu.Using flow cytometry to detect the effect of petroleum ether extract on apoptotic cells.Using immunohistochemical method to observe the influence of this extract to the expression of Survivin and Bax(Bcl-2 associated protein x).[Result]The extract of petroleum ether of Rhizoma Amorphophalli had inhibitory effect of the growth on mice vaccinated with H22 tumor,and the possible mechanism was the downregulation of Survivin expression and upregulation of Bax expression to induce apoptosis of tumor cell.
ABSTRACT
[Objective]To determine the content of Polygonum multiflorum thunb. Polysaccharide and the refined. [Methods] The sulfuric acid-phenol method was used. [Results] ?_ max = 486nm. The linear rang was 10.5～94.5?g?L -1 .And the average recovery was 101.23%(n=5,RSD=3.03%). The content of Polygonum multiflorum thunb. Polysaccharide was 14.28% and the refined was 19.03%. [Conclusion] The method was simple,rapid and reliable.
ABSTRACT
Metabonomics is a new method for identifing the relationship between organism metabolites and the effect of external stimulates.It provides a new strategy and platform for the TCM study and the modernization of TCM since the main idea of metabonomics is in accordance with that of TCM.
ABSTRACT
To establish and observe rabbit hyperlipemia and atherosclerosis model, we combined the method of high-lipid diet and immuoreactive injure. We divided 45 New Zealand rabbit into control group and high-lipid group. According to the time (4, 8, 12 weeks) of established model, the control group and high-lipid group were divided into 3 groups respectively. The blood-lipid, the hemodynamics parameter and vascular intima were determined and observed. The results showed: (1) After feeding 4, 8, 12 weeks, TC and LDL-C of the high-lipid group in the serum increased obviously. After feeding 8 week, TG of the high-lipid group began to increase obviously. HDL-C of the high-lipid group was higher than control group, but with a descendent trend. (2) Blood viscosity of the high-lipid group increased obviously under the 0.512 S(-1) and 5.96 S(-1) at 12 week. Blood flow increased obviously at 8 and 12 week. SBP increased evidently at 8 and 12 week. Alteration of the plasma viscosity and vascular diameter were not obvious. (3) By observing with optical microscope the intima of the control group is smooth. The intima of the high-lipid group has a light incrassation at 4 week. The intima of the high-lipid group has a obvious incrassation at 8 and 12 week. By observing with through transmission electron microscopy (TEM), the lipid vacuole is under the endothelium cell. (4) By adopting the immunohistochemistry, the foam cell that derived from smooth muscle cell were determined. We concluded that the blood lipid can have a prefigurative role in atherosclerosis; blood flow and blood pressure and blood viscosity increase at low shear rate in the course of the atherosclerosis; vascular intima is incrassate and the composition of the AS plaque is variational continually.
Subject(s)
Animals , Male , Rabbits , Atherosclerosis , Blood , Pathology , Endothelium, Vascular , Pathology , Hemodynamics , Hyperlipidemias , Blood , Pathology , Lipids , BloodABSTRACT
There are three key factors in tissue engineering: seeding cells, scaffold and their interaction. Although mesenchymal stem cells (MSCs) are potential seeding cells, the problem of what phase MSCs should be used is not yet solved. On the other hand, degradable porous scaffolds which have good mechanics and good biocompatibility are preferred. To choose the optimum seeding cells and the suitable ratio of beta-TCP/PLLA porous scaffold, we observed the phenotype of the male SD rat's osteoblastic MSCs and detected the amount of alkaline phosphatase, osteocalcin and type I collagen secreted by the osteoblastic rMSCs in different phase. About 10, 14 and 20 days after induction, the induced cells came into proliferative phase, matrix synthesis phase and mineralization phase, respectively. Then we chose the suitable cells and seeded them on beta-TCP/PLLA composite scaffolds with different ratios (beta-TCP/PLLA = 1:1; beta-TCP/PLLA = 1:2; and beta-TCP/PLLA = 2:1). Fluorescence microscope, scanning electron microscope and MTT assay were used to observe and to detect the biocompatibility of the scaffolds. The results indicated that all of these materials have biocompatibility to some extent. Cells can grow well on all of the scaffolds. However, scaffold beta-TCP/PLLA = 2:1 seems to be a more suitable tissue engineering scaffold on account of its minimal influence on cell growth and differentiation.
Subject(s)
Animals , Male , Rats , Biocompatible Materials , Calcium Phosphates , Pharmacology , Cell Differentiation , Cells, Cultured , Coculture Techniques , Lactic Acid , Pharmacology , Mesenchymal Stem Cells , Cell Biology , Osteoblasts , Cell Biology , Polyesters , Polymers , Pharmacology , Porosity , Rats, Sprague-Dawley , Tissue EngineeringABSTRACT
Objective:To study the extraction method of total flavonoids in Mosla hangzhowensis Matsuda.Methods:Orthogonal experiment design was used,with the content of the total flavonoids as the index,to study the effect of ethanol concentration,solvent quantity and extraction time on recovery rate of total flavonoids.Results:The best extraction condition was:extract two times;adding 20 fold 65% ethanol and to extract an hour each time.Conclusion:This extraction method is convenient and feasible.