ABSTRACT
OBJECTIVE：To optimize the formulation of Curcumin （CUR）transethosomes（CUR-TEs）. METHODS ：The contents of CUR in CUR-TEs were determined by HPLC. CUR-TEs were prepared by injection method. Using comprehensive score of encapsulation efficiency and drug loading as index ，based on signal factor test ，Box-Behnken design-response surface method was used to optimize and validate the formulation. The property of CUR-TEs prepared by the optimal formulation was investigated. RESULTS：The optimal formulation of CUR-TEs was as follows as lecithin of 4％，CUR of 0.13％，1，2-propylene glycol of 25％，tween-80 of 1％. Results of validation test of optimal formulation showed that comprehensive score of encapsulation efficiency and drug loading of CUR-TEs was 93.04±2.16，relative error of which to predicted value （91.19）was 2.03％. The encapsulation efficiency of CUR-TEs prepared by optimal formulation was （91.17±1.35）％，and its drug loading was （0.94± 0.02）％. The particle size was （190.64±15.97）nm with polydispersity index of 0.086±0.007，and Zeta potential was （－12.74± 1.60）mV. CONCLUSIONS ：The optimized formulation of CUR-TEs is stable ，feasible and repeatable ，with good stability.
ABSTRACT
A novel teaching pattern of the experimental course of pharmaceutics has been carried out in undergraduate teaching since 2006.Under the experimental teaching pattern,students were activated to initiatively enjoy the interest of scientific research and exploration.The new teaching pattern provided students with good chance for the cultivation of their creative thinking and comprehensive quality.
ABSTRACT
OBJECTIVE:To study the preparation technique for oral vaccine microspheres.METHODS:Newcastle dis-ease virus(NDV)vaccine was used as model drug,and PLGA as carrying agent to prepare NDV-PLGA oral microspheres by W/O/W emulsifying-solvent evaporation technique.Orthogonal design experiment was carried out to determine the influence of NDV volume,PLGA concentration,stirring speed and protectants at inner aqueous phase on the shape,size,adhesion and activity of microspheres.The valence of drug release in microspheres was measured by hemagglutination.RESULTS:The best condition for preparing NDV microspheres was found to be200?l of NDV,4%PLGA,stirring speed at8000r/min,and BSA as protectant.CONCLUSION:The established technique is the optimal choice for preparation of oral vaccine micro-spheres.