ABSTRACT
Objective @#To study the association between single nucleotide polymorphism (SNP) in transcriptional coactivator containing PDZ binding motif (TAZ) gene and the risk of non⁃cardia gastric cancer. @*Methods @#Among 460 patients with non⁃cardia gastric cancer and 470 normal controls , TAZ rs16861985 , rs1055153 , rs6783790, rs12490239 were genotyped by polymerase chain reaction⁃restriction fragment length polymorphism (PCR⁃RFLP) . Unconditional Logistic regression was used to evaluate the association of studied SNPs with the risk of non⁃cardia gastric cancer. @*Results @#TAZ rs16861985 was associated with the risk of non⁃cardia gastric cancer, compared with the CC genotype carriers , the carriers of GC genotype had an increased risk of noncardia gastric cancer ( OR =1. 547 , 95% CI: 1. 145 - 2. 089) . TAZ rs1055153 was associated with the risk of non-cardia gastric cancer, compared with the GG genotype carriers , the carriers of GT + TT genotypes had a decreased risk of non-cardia gastric cancer ( OR = 0. 570 , 95% CI: 0. 400 - 0. 814) . TAZ rs6783790 and rs12490239 were not associated with the risk of non⁃cardia gastric cancer. Among the haplotypes constructed by TAZ rs16861985 , rs1055153 , rs6783790 and s12490239 , C ⁃G ⁃A ⁃A haplotype had a decreased risk of non⁃cardia gastric cancer ( OR = 0. 761 , 95% CI: 0. 612- 0. 945) , while G ⁃G ⁃A ⁃G haplotype had an increased risk of non⁃cardia gastric cancer ( OR = 1. 894 , 95% CI:1. 314 - 2. 730) . The fourth⁃order interaction of TAZ rs16861985 , rs1055153 , rs6783790 and rs12490239 was associated with non⁃cardia gastric cancer risk(P < 0. 05) .@*Conclusion @#TAZ rs16861985 and rs1055153 may play a role in the risk of non⁃cardia gastric cancer. The C ⁃G ⁃A ⁃A haplotype constructed by TAZ rs16861985 , rs1055153, rs6783790 and rs12490239 can reduce the risk of non⁃cardia gastric cancer, and G ⁃G ⁃A ⁃G haplotype can increase the risk of non⁃cardia gastric cancer. The interaction of TAZ rs16861985 , rs1055153 , rs6783790 and rs12490239 has a synergistic effect innon⁃cardia gastric cancer.
ABSTRACT
Objective To investigate whether the aberrantly expressed HLA class Ⅱ molecules on the follicular epithelial cells of Graves' disease and Hashimoto's thyroiditis present autoantigens. Methods Expressions of HLA class Ⅱ, invariant (Ii) chain and B7 on follicular epithelial cells were detected by labeled streptavidin binding (LSAB) and expression of Ii chain at mRNA level was assayed by in situ hybridization (ISH). Results The expression pattern of HLA class Ⅱ, Ii chain and B7 on epithelial follicular cells from Graves' disease and Hashimoto's thyroiditis is highly heterogeneous. Conclusion Follicular epithelial cells possess the necessary molecular basis for presenting autoantigens and activating T cells, at least in a part of the patients.