ABSTRACT
At present, the medical mode has changed from bio-psycho-social medical model to "4 P" medical mode. Therefore, it is necessary to fully consider the individual needs of patients, treat patients equally, adheres to the principle of faith and compliance, and adhere to the humanistic nursing concept. To prevent nurse-patient disputes, we should start from three aspects. Firstly, we should reform the current model of nurse education and training, reform the content of education and attach importance to moral sentiment education. Secondly, we should strengthen the cooperation between doctors and nurses, improve the level of health education, and build a harmonious nursing environment and a social atmosphere of respecting medical staff. Finally, we call for strengthening medical legislation and protecting the legitimate rights and interests of nurses with legal weapons.
ABSTRACT
Objective To compare the antitumor effects and chemical components of the extracts in cultivated Cordyceps sinensis (Cs) fungus HK-1 and natural Cs. Methods The cultivated Cs fungus HK-1 and natural Cs were extracted with petroleum ether (PE), ethyl acetate (EtOAc), ethanol (EtOH), and water. The cytotoxicity of all the extracts was observed with MTT assay on breast cancer cell line MCF-7, mouse melanoma cell line B16, human premyelocytic leukemia cell line HL-60, and human hepatocellular carcinoma cell line Hep G2. The antitumor activity in vivo of the extract was further studied with animal model. The chemical constituents in the extracts were analyzed by HPLC. Results All of the extracts from the cultivated Cs fungus HK-1 had much stronger cytotoxicity on B16 than those from natural Cs, and the EtOAc extract had the most potent activity. The activity of EtOAc extract from the cultivated Cs fungus HK-1 was more potent than that from natural Cs on all the four cancer cell lines. EtOAc extract from the cultivated Cs fungus HK-1 also can inhibit the growth of tumor in vivo. Chemical components of all extracts were analyzed and ergosterol and adenosine were found to be potential compounds. Conclusion The cultivated Cs fungus HK-1 has stronger antitumor activity than natural Cs and is a potential source of antitumor compounds.