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Objective@#To analyze the epidemiological characteristics and response process of an acute poisoning event caused by carbofuran in buttered tea and provide scientific evidence for the investigation of similar events in the future.@*Methods@#Field epidemiological survey, animal experiments and laboratory tests were conducted for an acute poisoning event occurred in Suopo township of Danba county of Sichuan province in 2018. Descriptive epidemiological method was used to analyze the epidemiological characteristics of the acute poisoning event.@*Results@#A total of 26 poisoning cases occurred in 3 villages. The total attack rate was 41.27%. No death cases were reported. The 26 cases occurred in a few minutes after drinking buttered tea, the main symptoms were vomit, dizziness, miosis and nausea. A dog showed the same symptoms after drinking a sample of buttered tea. Carbofuran was detected in buttered tea, vomitus and zanba samples.@*Conclusions@#The acute poisoning was caused by carbofuran in buttered tea, the transmission mode was point source spread. Effective epidemiological investigation and simple animal experiment can provide evidence for the rapid sample detection and clinical treatment of cases in emergency response. Timely case treatment and strict poisoning source control are the key measures to reduce casualty and prevent the spread of poisoning.
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Objective To explore and compare the clinical curative effect of tocilizumab and leflunomide in the treatment of rheumatoid arthritis and to evaluate the safety. Methods From March 2015 to March 2016,70 cases of rheumatoid arthritis treated in the department of rheumatism in this hospital were divided into treatment group and control group by using a random number table method,35 cases in each group.The patients in the observation group were intravenously treated with tocilizumab,at dosage of 8 mg·kg-1,once every four weeks.The control group was treated by oral administration of leflunomide tablets,at 50 mg·d-1from the 1st to 3rd day,and at 20 mg·d-1from the fourth day to the end of the treatment.The treatment period was 24 weeks in the two groups.Joint swelling,joint pain,morning stiffness,ESR,CRP,IL-6 and IL-8 were recorded and compared before and during the treatment.Total effective rate of treatment was compared between the two groups.Adverse drug reaction was recorded and the incidence of adverse drug reactions was compared. Results After the treatment,joint swelling, joint pain,morning stiffness,ESR,CRP,IL-6 and IL-8 were significantly lower in the treatment group than those in the control group (P<0.05).After the treatment,the total effective rate of the treatment was significantly higher than that of the control group (P<0.05).After the treatment,incidence of adverse reaction was significantly lower in the treatment group than that in the control group(P<0.05). Conclusion Tocilizumab and leflunomide has certain curative effect in the treatment of rheumatoid arthritis, but tocilizumab is more effective,with low incidence of adverse reaction and a high clinical value.
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Objective To investigate the effects on proliferation of bone marrow mesenchymal stem cells (MSCs) by recombinant human granulocyte colony-stimulating factor in mice. Methods Kunming mice were randomly divided into G-CSF and control groups (n=15). The mice were subjected to subcutaneous injections of rhG-CSF at a dose of 80 ?g/kg per day and control of saline for 5 days. The bone marrow was obtained on 6th, 12th and 168th h respectively after the final administration. The MSCs were separated and cultured, and the colony-forming unit-fibroblast (CFU-F) was evaluated. The cell cycle and the surface antigens were analyzed by flow cytometry. Results The number of CFU-F was increased after administration of the rhG-CSF (P 0.05). Flow cytometic detection of MSCs surface marks in fibroblast colony showed CD34~ -, CD133~ -, CD90~ + and CD105~ +, with the percentage of 2.5 %, 3.1 %, 67.0 % and 78.0 %, respectively. After mobilization with rhG-CSF, the percentage of G_0/G_1 phases in bone marrow MNCs was decreased (P