ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of 2 different ways of storage bag placement on some biochemical indexes of leukodepleted red blood cells (LD-RBC) to as to ensure the efficacy and safety of clinical blood transfusion.</p><p><b>METHODS</b>The whole blood samples of 20 donors (400 ml/donor) were selected for preparating the LP-RBC, which were divided evenly into 10 bags. The 10 bags were randomly divided into 2 groups; the bags in 1 group were placed uprightly, while the bags in another group were placed horizontally. The bags of 2 groups were stored in the same conditions. One storage bag from each group was taken randomly on day 7, 14, 21, 28, 35 respectively, and then the biochemical indexes of samples were detected and analyzed.</p><p><b>RESULTS</b>The values of K(+) and LAC on day 14, the value of LDH on day 28 in the uprightly placed group were higher than those in the horizontally placed group (P < 0.05), the value of Na(+) on day 28, and the value of Glu on day 35 in the uprightly placed group were lower than those in horizontally placed group (P < 0.05), but there was no significant difference in Cl(-) level between 2 groups (P > 0.05).</p><p><b>CONCLUSION</b>The storage bags placed by different ways during the storage show different influence on some biochemical indexes of LD-RBC in the storage period.</p>
Subject(s)
Humans , Blood Specimen Collection , Methods , Blood Transfusion , Erythrocytes , Random AllocationABSTRACT
We isolated a high efficient antifungal strain A02 from forest soil in a suburb of Beijing. The result of polyphasic taxonomy confirmed that strain A02 belongs to Streptomyces lydicus. The fermented broth of the strain presented a stable and strong inhibiting activity against many plant pathogenic fungi. The purpose of this study was to ascertain the substance base of the antifungal activity of strain A02. We extracted the antifungal metabolite of A02 by using column chromatography with X-5 macroporous resin and 100-200 mesh silica gel respectively, and then purified it by LC-9101 recycling preparative HPLC with a SP-120-15 column (JAIGEL-ODS-AP). An active compound with purity over 99.845% was finally obtained. The chemical structure of the active compound was determined with spectroscopy methods, including ultraviolet spectrometry, infrared spectrometry, high resolution mass spectrometry and nuclear magnetic resonance. According to the analysis results, we identified the active compound as a tetraene macrolide antibiotic with the molecular weight of 665, the molecular formula C33H47No3 and the same chemical structure as natamycin. Our research revealed a new biosynthetic function for S. lydicus to produce natamycin, and an expanding application field for natamycin to be used for the control of fungal plant diseases.
Subject(s)
Antifungal Agents , Chemistry , Natamycin , Soil Microbiology , Streptomyces , Chemistry , MetabolismABSTRACT
0);while the gene frequency of HLA-DQA1 * 0401 allele in children FC was 0.9 %,which was lower than that of the control group(8.5 %,P = 0.0350).Conclusion HLA-DQA1 0101 allele maybe a susceptible gene and HLA-DQA1 * 0401 allele maybe a protective gene of FC in children FC in Han nationality in Baotou.There was no correlation between HLA-DQB1 and FC.