ABSTRACT
Objective To research the mechanism of TX0201 abstracted from Heart-Regulating Formula in treatment of rats with Alzheimer's disease (AD). Methods The rat model of AD was induced by A?25-35 injected into the bilateral amygdale. To observe the spatial learning and memory ability, by means of RT-PCR and immunohistochemistry analysis, the expression of ?-amyloid precursor protein (APP), glial filament acid protein (GFAP) in the brain of the model animal were examined. Results In AD model group, the spatial learning and memory ability was damaged significantly, the expression of APP mRNA increased in its cortex and hippocampus. GFAP immunopositive signal, and IL-6 mRNA in cortex and hippocampus and the expression of IL-1? mRNA in hippocampus were upregulated. TX0201 improved the spatial learning and memory disturbance, decreased the expression of APP mRNA and IL-1? mRNA in hippocampus, downregulated GFAP and IL-6 mRNA in cortex and hippocampus. Conclusions The model showed the characteristic of AD such as memory hypofunction. TX0201 could partially ameliorate memory function, decrease the expression of APP mRNA in hippocampus, alleviate inflammation-immunity reaction, that is one of the effect mechanisms of TX0201.
ABSTRACT
Many investigations showed that abnormal blood insulin level is associated with the pathogenesis of Alzheimer′s disease and its clinical manifestation, suggesting that increasing blood insulin level and/or improving insulin sensitivity seem to be helpful for patients with Alzheimer′s disease.