ABSTRACT
Objective To investigate the inhibitory effect of melatonin on the migration and invasion of gastric cancer cell and the underlying molecular mechanism. Methods Human gastric cancer SGC-7901 cells were treated with different concentrations of melatonin ( 0. 1, 1, 2 and 4 mmol/L ) for 24 hours, and the changes in the migration and invasion of gastric cancer cells were detected by scratch test and Transwell assay. The expressions of matrix metalloproteinase ( MMP) -2 and MMP-9 in the supernatant were detected by ELISA kit. The changes of MMP-2, MMP-9, intercellular cell adhesion molecule-1 ( ICAM-1 ) and CD44 expressions were detected by using Real-time PCR. The protein expressions of ICAM-1, CD44, p-P38, P38 and phosphorylated mitogen-activated protein kinase kinase ( p-MKK) 3/6 were detected by Western blotting. Results Melatonin inhibited the migration and invasion of gastric cancer cells in a dose- dependent manner. Compared with the blank control group, melatonin reduced the expression of MMP-2, MMP-9, ICAM-1 and CD44, and inhibited the expressions of p-P38, P38 and p-MKK3/6 in gastric cancer cells. Conclusion Melatonin inhibits the migration and invasion of gastric cancer cells by inhibiting the expressions of MMP-2, MMP-9, ICAM-1 and CD44. Inhibition may be related to the p38MAPK signaling pathway.
ABSTRACT
In order to investigate the role of melatonin in inhibiting the proliferation of murine gastric cancer and the underlying molecular mechanism, we performed an in vivo study by inoculating murine foregastric carcinoma (MFC) cells in mice, and then tumor-bearing mice were treated with different concentrations of melatonin (i.p.). The changes of Bcl-2, Bax, p21 and p53 expressions in tumor tissue were detected by using real-time fluorescence quantitative RT-PCR and Western blot. We found that: (1) melatonin resulted in reductions of tumor's volume and weight in the gastric cancer-bearing mice and thus showed anti-cancer effect; (2) melatonin reduced Bcl-2 expression, but increased the expression of Bax, p53 and p21 in tumor tissue. Our results suggest that melatonin could inhibit the growth of tumors in gastric cancer-bearing mice through accelerating the apoptosis of tumor cells.
Subject(s)
Animals , Mice , Apoptosis , Melatonin , Pharmacology , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Stomach Neoplasms , Drug Therapy , Metabolism , Tumor Suppressor Protein p53 , Metabolism , bcl-2-Associated X Protein , MetabolismABSTRACT
Objective To evaluate the curative effect of CT-guided stereotactic and neuroendoscope-assisted operation on cerebral hemorrhage caused by hypertension. Methods Twenty-five cases of cerebral hemorrhage caused by hypertension treated in our department in 2005-2006 were selected. ASA602A type position finder was installed under local anesthesia, and operation planning system was imported after CT scanning. The target point was selected at 1-1.5 cm posterior the central point of the largest hematoma, then the coordinate of the target point was calculated, and incisal opening and operation approach were programmed. Brain operation with trephine was performed under general anesthesia. After hematoma evacuating cannula were placed in the skull hole of 3 cm in diameter to remove partial hematoma, neuroendoscope was inserted to get rid of hematoma and stop bleeding.Results Of 25 patients with cerebral hemorrhage, 24 h after operation, 10 received evacuation of 60%-70% of hematomas, 11 did 80%-90%, 4 did more than 90%; 72 h after operation, all patients received evacuation of more than 90%. During the follow-up for 3 months after the operation, activities of daily living (ADL) scoring showed excellent improvement in 14 cases and amelioration in 9; 2 patients died from lung infection 10, 14 d after operation, respectively. Conclusions CT-guided stereotactic and neuroendoscope-assisted surgery is a safe, effective and minimally invasive method to treat hypertensive cerebral hemorrhage.
ABSTRACT
Objective To study the clinical outcomes of diffuse axonal injury(DAI) Methods A retrospective analysis was conducted involving 105 patients with DAI treated with comprehensive therapies.Results In the follow-up for 6 to 30 months,23 patients died,and among the 82 patients survived,6 wefe in vegetative state,19 had severe disabilities,37 had moderate disabilities,and 20 showed good recovery. Conclusion Comprehensive treatments including early use of hormones,hypothcrmic therapy,and application of hyperbaric oxygen and nimodipine can improve the prognosis of DAI.