ABSTRACT
<p><b>OBJECTIVE</b>Matrix metalloproteinase 1 (MMP1) plays an important role in the development of lung cancer. This study was to investigate the relation to associate the single nucleotide polymorphism(SNP)in MMP1 gene with the susceptibility to lung cancer in Northwestern Chinese population of Han nationality.</p><p><b>METHODS</b>By using the methods of polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), MMP1 -1607(1G>2G) polymorphisms in 150 patients with lung cancer, and 200 healthy controls were detected to evaluate the relationship between different genotypes and susceptibility of lung cancer.</p><p><b>RESULTS</b>Individuals with 2G/2G genotype had 1.77 fold risk suffering from lung cancer, when compared with ones with 1G/2G and 1G/1G genotypes. Smokers with 2G/2G genotype exhibited 3.20 fold elevated risk for lung cancer (OR 3.20; 95% CI 1.50-6.82).</p><p><b>CONCLUSION</b>The -1607(1G>2G) in promoter region of MMP1 is associated with susceptibility to lung cancer in Northwestern Chinese population of Han nationality. The genotype 2G/2G enhances the susceptibility to lung cancer.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Asian People , Genetics , Base Sequence , China , Genetic Predisposition to Disease , Genetics , Lung Neoplasms , Genetics , Matrix Metalloproteinase 1 , Genetics , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Genetics , Sequence Analysis, DNAABSTRACT
<p><b>OBJECTIVE</b>To detect the factors relevant to stenosis of tracheal graft and to find feasible methods to solve this problem.</p><p><b>METHODS</b>Sixteen mongrel dogs were divided into groups A and B randomly and equally. Five-ring-length tracheal segments were allotransplanted. All grafts and anastomotic sites were covered with omental pedicles. In group A, no immunosuppressant was given and in group B, the recipients were treated with cyclosporine. The animals were sacrificed 4 weeks after operation, and their postmortem specimens were examined grossly and histologically. All allografts were assessed by percent patency. Epithelial regeneration and morphology of the cartilage were semiquantitatively evaluated.</p><p><b>RESULTS</b>Structural integrity of the allografts were maintained better in group B than in group A. Tracheal stenosis was found to be more serious in group A. The scores of epithelial regeneration and cartilage morphology were higher in group B than in group A, and in each group positive correlation was found between the percent patency and the score of epithelial regeneration or cartilage morphology.</p><p><b>CONCLUSIONS</b>Immunosuppressive drugs are necessary to maintain the structure of allografts. Tracheal stenosis is correlated closely with epithelial regeneration and morphological maintenance of the cartilage.</p>