ABSTRACT
Purpose Liver fibrosis,liver cirrhosis and liver cancerseriously threaten human health.To detect liver diseases by specific nanoprobes,and to provide reference for the formulation of treatment strategies.Materials and Methods In this study,bovine serum albumin was used as a skeleton and modified with galactose groups for targeting asialoglycoproteinreceptors on liver.The albumin was labeled with radioactive iodine by chloramines T method,and indocyanine green molecules was encapsulated to form liver-targeting nanoparticles.Physical and chemical characterization (particle size and spectral characterization),bio-distribution,SPECT imaging and photoacoustic imaging were carried out respectively.Results The size of the nanoparticles was about 86.4 nm and there were two obvious absorption peaks at 705 nm and 780 nm.Bio-distribution showed that the radiolabeled nanoparticles had a high distribution in liver at 60 min [(55.52 ± 5.39)％ID/g],while after the receptor was inhibited,the uptake in liver was reduced to (37.01 ± 7.38)％ID/g,indicating a significant inhibitory effect (P＜0.05);the uptake of this material at 240nm still remained (34.22±4.44)％ID/g,while in other organs,the detected uptake was quite low,with a statistically significant difference (P＜0.05).The results of SPECT and photoacoustic imaging were consistent with the data of bio-distribution,and images showed highest signal on liver.Thus,the probe was suitable for liver imaging.Conclusion 131I-labeled albumin nanoparticle is an excellent liver-targeting dual modal imaging probe,which can be used for the detection of liver diseases.