ABSTRACT
Objective To observe the effects of Yiqi Huoxue Tuodu Prescription on Keap1Nrf2/HO-1 signaling pathway in rats with chronic nonbacterial prostatitis(CNP);To explore its mechanism for the treatment of CNP.Methods CNP rat model was prepared using castration combined with estrogen induction method.Totally 48 SD rats were divided into blank group,model group,celecoxib group and Yiqi Huoxue Tuodu Prescription group according to the random number table method,with 12 rats in each group.In the celecoxib group,celecoxib suspension was instilled 0.035 g/kg,and in the Yiqi Huoxue Tuodu Prescription group,Yiqi Huoxue Tuodu Prescription water decoction was instilled 8.64 g/kg,and the blank group and the model group were instilled with equal volume of normal saline for 28 days.Mechanical pain threshold in rats was measured using Von Frey fiber optic pain gauge,HE staining was used to observe pathological changes in prostate tissue and pathological scoring,the content of reactive oxygen species(ROS)in prostate tissue were detected by chemical fluorescence method and the glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)content in prostate tissue were detected by colorimetric method,Western blot was used to detect the expressions of Kelch like ECH related protein 1(Keap1),nuclear factor E2 related factor 2(Nrf2),and heme oxygenase-1(HO-1)protein in prostate tissue.Results Compared with the blank group,rats in the model group had significantly lower mechanical pain threshold and significantly decreased prostate index(P<0.01);the size of the glandular cavity in prostate tissue varied,with the disappearance of secretions in the cavity,interstitial looseness and edema,a large amount of fibrous tissue hyperplasia and inflammatory cell infiltration,and a significant increase in pathological scores(P<0.01);the contents of ROS and MDA in prostate tissue significantly increased,the activity of GSH-Px significantly decreased(P<0.01),the expression of Keap1 and Nrf2 proteins significantly decreased,and the expression of HO-1 protein significantly increased(P<0.01,P<0.05).Compared with the model group,the mechanical pain threshold of the rats in the Yiqi Huoxue Tuodu Prescription group was significantly higher(P<0.01);there was mild damage to prostate tissue,with a small amount of fibrous hyperplasia and inflammatory cell infiltration,and a significant decrease in pathological scores(P<0.01,P<0.05);the contents of ROS and MDA in prostate tissue significantly decreased,and the GSH-Px activity significantly increased(P<0.01),the Keap1 and Nrf2 protein expressions significantly increased and HO-1 protein expression significantly decreased in prostate tissue(P<0.01,P<0.05).Conclusion Yiqi Huoxue Tuodu Prescription can effectively improve the histopathological morphology and increase the pain threshold of the prostate gland in CNP rats,and its mechanism of action may be related to the regulation of Keap1/Nrf2/HO-1 signaling pathway and reduction of oxidative stress damage in prostate tissue of rats.
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Objective To analyze the relevant risk factors for Chinese adult urinary lithiasis.Methods We searched the PubMed,Embase,Cochrane Library databases,the Chinese Biomedical Literature Database,VIP Database,China Knowledge Network (CNKI) database,Wanfang database by computer.The search was set for all case-control studies on urinary lithiasis with Chinese patients.Two authors independently screened the literature,extracted literature data,and evaluated the quality of the literature.The software Revman 5.3 was used to perform meta-analysis in this study.Results A total of 25 studies in this study were case-control studies,with a total of 8 524 cases of urinary lithiasis and 42 239 cases of non-urinary lithiasis.A total of 9 risk factors of urinary lithiasis were screened:low intake of water [OR =2.64(95％CI 2.00-3.48),P ＜0.001],overweight [OR =2.36(95％ CI 1.31-4.23),P =0.004],tobacco consumption[OR =2.10(95％ CI 1.26-3.48),P =0.004],high salt diet [OR =1.88 (95％ CI 1.21-2.91),P =0.005],alcohol consumption [OR =1.85 (95％ CI 1.57-2.17),P ＜ 0.001],low vegetable consumption [OR =1.64 (95 ％ CI 1.05-2.54),P =0.03]、high protein diet [OR =1.49(95％ CI 1.23-1.82),P ＜0.001],low fruit consumption [OR =1.39(95％ CI 1.26-1.53),P ＜0.001] and tea consumption [OR=1.23(95％CI1.10-1.37),P＜0.001].Conclusions Lowintake of water,overweight,tobacco consumption,high salt diet,alcohol consumption,low vegetable consumption,high protein diet,low fruit consumption and tea consumption could be the risk factors for chinese adult urinary lithiasis.
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Objective To investigate the surgical management of atrophic gallbladder.Methods The clinical data of 85 patients with atrophic gallbladder surgically treated in our hospital from October 2002 to October 2005 were retrospectively analyzed.Results There were 3 cases of iatrogenic bile duct damage,2 of enteral damage,1of post operative remaining small gall bladder,1 of remain ingstonesinthe bile duct and 1 of gallstone ileum.Two 2 patients died after operation.Conclusion Atrophic cholecystitis is always accompanied by many complex clinical problems.Adoption of proper surgical methods can lower the incidence of postoperative complications.