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Objective:To compare the clinical outcomes between modified percutaneous kyphoplasty (PKP) and conventional PKP in the treatment of Kümmell disease.Methods:A retrospective cohort study was conducted of the 57 patients with single level kummell disease who had undergone PKP between January 2015 and December 2017 at Department of Spinal Surgery, Jiangmen Hospital Affiliated to Sun Yat-sen Uni-versity.Of them, 36 received modified PKP and 21 conventional PKP.In the modified PKP group, there were 24 males and 12 females with an age of 73.9 years±9.4 years and a course of disease of 2.2 months±1.5 months; the disease was located at a thorocic vertebra in 20 cases and at a lumbar vertebra in 16.In the conventional PKP group, there were 15 males and 6 females with an age of 72.6 years±11.9 years and a course of disease of 1.8 months±1.1 months; the disease was located at a thorocic vertebra in 10 cases and at a lumbar vertebra in 11.The 2 groups were compared in terms of operation time, bone cement volume, cement leakage, visual analogue scale (VAS), Oswestry disability index (ODI), anterior and middle heights and cobb angle of injured vertebra at postoperative 3 days, 1 and 3 months, and final follow-up.Results:There were no significant differences in the general clinical data between the 2 groups, showing compatibility between groups ( P>0.05).All the operations were accomplished uneventfully with no such serious complications as spinal cord nerve injury, wound infection, pulmonary embolism, bone cement poisoning reaction or death. There was no significant difference between the 2 groups in operation time or bone cement volume ( P>0.05).The rate of cement leakage in the modified PKP group [33.3%(9/27)] was significantly lower than that [52.4%(11/21)] in the conventional PKP group ( P<0.05).There were no significant differences be-tween the 2 groups in VAS, ODI, anterior or middle height of injured vertebra or cobb angle at postoperative 3 days or one month ( P>0.05), but the postoperative values were improved compared to the preoperative ones.At postoperative 3 months and final follow-up, the modified PKP group was significantly better in all the indexes than the conventional PKP group ( P<0.05). Conclusion:Compared with conventional PKP, the modified PKP may lead to better outcomes for Kümmell disease.
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Objective To briefly retrospect the production and quality control of 64 Cu(n = 9) in Department of Nuclear Medicine of Peking University Cancer Hospital in order to provide useful information for the further production and application of this novel radionuclide. Methods 64 Ni(p, n) 64 Cu nuclide re-action was used for the 64 Cu production. Firstly, a new electro-planting device for 64 Ni planting was de-signed. HM-20 cyclotron was applied to irradiate the slice for 5-8 h. 64 CuCl2 was purified, collected and in-jected into normal mice. MicroPET was conducted to monitor the metabolism in vivo. Results A new type of electro-planting device was designed and assembled. The enriched 64 Ni target showed smooth, even, dense surface and without obvious pits and cracks. High specific 64 Cu (1.3-4.1 GBq) can be collected after radio-chemical purification. 64 Cu was finally dissolved in 0.01 mol/ L HCl with high radionuclide purity (over 99.97％). MicroPET of 64 CuCl2 in normal mice showed that the radioactivity was mainly accumulated in the liver. Conclusion A new and real-time observable device for 64 Ni electro-plating has been designed and successfully used in the production of 64 Cu with high specific activity.
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Objective To prepare 68Ga-PSMA-617 and perform its microPET imaging on both normal BALB/c mice and BGC-823 (PSMA expression) tumor bearing mice.Methods 68GaCl3 was eluted from 68Ge-68Ga generator by 0.05 mol/L HCl,then added to the DKFZ-PSMA-617 and heated at 85 ℃ for 5 min.The labeling efficiency and in vitro stability of 68Ga-PSMA-617 in sodium chloride solution and HAS were analyzed by radio-HPLC.Water partition coefficient and plasma protein binding rate were also evaluated.MicroPET imaging was performed in normal female BALB/c mice and human gastric tumor (BGC-823) bearing mice at 60 min post-injection of 68Ga-PSMA-617.18F-FDG was also injected to BGC-823 tumor bearing mice to acquire microPET imaging for contrast.Results The labeling yield of 68Ga-PSMA-617 was 97.9％,and it could be used directly without purification.68Ga-PSMA-617 showed good in vitro stability in sodium chloride solution and 5％ HAS,the radiochemical purities were 94.9％ and 81.0％ respectively at 80 min post-incubation.68Ga-PSMA-617 was water-solubility substance,and it cleared mainly through the kidneys.MicroPET imaging showed that 68Ga-PSMA-617 could be accumulated in tumor (T/NT=2.28),which was better than 18F-FDG.Conclusions Preparation of 68Ga-PSMA-617 is convenient and has a high labeling yield.It can specifically target to PSMA expression tumors and has a promising prospect in clinical application.
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The combination of biochemistry analyzer and medical expert system was proposed in this report. Biochemistry analyzer is one of the most important analytical instrument used in clinical detection. It could take immunological examination and biochemical analysis for blood, urine, pleural effusion and cerebrospinal fluid and other body fluids. Medical expert system is an intelligent program system with knowledge and experience of a large number of medical specialists. It could use the knowledge and method of medical experts to solve and deal with problems in the field. This system mainly includes human interface, inference engine, interpreter, knowledge acquisition procedures, integrated database and knowledge base. Some parts of system design, such as the expression and design, and interpretation mechanism of the knowledge base, have been interpreted in details. It adopts production as an expression of knowledge. Generally, knowledge was expressed as if the conditions, then the conclusion form. Interpretation mechanism use the error counter propagation of neural network to train the algorithm (BP algorithm for short).The combination could automatically conduct comprehensive analysis of various data generated by the instruments, and then obtain the science theoretical foundation and the most reasonable specialist conclusions. This report provides an overview of the system design of medical expert system.
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The erythropoietin-producing hepatocellular receptor (Eph)B4 receptor is closely associa-ted with tumor growth and angiogenesis,which is over-expressed in a wide variety of tumors.Molecular probes targeted for EphB4 receptor can improve the accuracy and specificity of tumor diagnosis.A lot of molecular probes targeted for EphB4 receptor have been designed,which are expected to provide new means for the early diagnosis and therapeutics of tumors.
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Objective:To design the liquid level detection system by ultrasonic method to meet the requirements of automatic enzyme immunoassay workstations and have a precise detection of liquid level. Methods:Because the existing sample needle should move down, liquid surface and the poor conductivity of liquid were invalid. So a method of ultrasonic liquid level detection system was designed. The control signal was launched by a microprocessor to control ultrasonic signal generator chip and the ultrasonic signal reflected when the liquid level in a test tube or microplate. Results: By receiving the reflection echo signal sent to the microprocessor for signal processing, the liquid level was detected. Conclusion: The ultrasonic method applied to the liquid level detection system to overcome the above shortcomings, improve pipetting accuracy and avoid cross pollution, so as to meet the requirement of the automatic enzyme immunoassay workstation test.
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Objective To evaluate the influence of the single and multiple intervertebral space radiofrequency ablation nucleoplasty on the sheep cervical spine stability.Methods Twenty healthy adult sheep were randomly divided into single intervertebral space operation group (A group )and multiple intervertebral space operation group ( B group ),each group was subdivided into postoperative 24 hours group ( A1,B1 group ) and post- operative 3 months group ( A2,B2 group),each group had five sheep.Radiofrequency ablation nucleoplasty on sheep C3/4 in the single intervertebral space operation group or C3/4,C4/5 in multiple intervertebral space operation group.Preoperative cervical vertebrae roentgenograms from all samples in neutral,lateral,hyperextension and hyperflexion positions were collected,and 5F pipe was used as the survey mark of ieonography.For the postoperative 24 hours group( A1,B1 group ),the roentgenograms were collected postoperatively 24 hours later,while for post- operative 3 months group (A2,B2 group),they were collected postoperatively 3 months later.The height of operative intervertebral space,horizontal and angular displacement of neighboring vertebral body were measured respectively.Results The roentgenograms showed no any obvious decrease in the height of intervertebral disc and no any increase in horizontal and angular displacement of neighboring vertebral body were observed in single intervertebral space operation group (A group) and multiple intervertebral space operation group (B group ).There was no significant difference between pre - operation and post - operation in them ( P ＞ 0.05 ).Conclusions The stability of the sheep cervical spine had not been affected by the single or multiple intervertebral space radiofrequency ablation nucleoplasty on the basis of X-ray results.The radiofrequency ablation nucleoplasty had no influences on the stability of sheep cervical vertebrae.