ABSTRACT
Objective To investigate the expressions of PGE2 and COX-2 between colorectal cancer tissue and normal colorectal mucosa tissue,and its relation to clinical pathology and significance.Methods 115 colon cancerous tissues and 69 normal colon tissues were collected in our hospital from Jan 2010 to Sept 2011.Immunohistochemistry was used to detect the PGE2 and COX-2 expression in both of the colorectal cancer and normal tissues.Results Positive expressions of PGE2 and COX-2 were 87.8％ (101/115) and 80.9％ (93/115) in the colorectal cancer tissue,and 8.7％ (6/69),21.7％ (15/69) in normal colorectal mucosa tissue respectively (x2 =110.96,62.194,all P ＜ 0.05).Expressions of PGE2 and COX-2 were closely associated with,tumor invasion,differentiation,lymph node metastasis and TNM stage (P ＜ 0.05).The Spearman rank correlation analysis indicated that the PGE2 and COX-2 expressions in tissues were closely correlated (r =0.987,P ＜ 0.05).Survival rate of the patients in both negative expressions of PGE2 and COX-2 (63.6％) were higher than that of both positive expressions (37.8％,P ＜ 0.05).Conclusion High PGE2 and COX-2 expressions in colorectal cancer tissues predicts poor patients' survival.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of colon cancer cell-derived interleukin-1α on the migration and proliferation of human umbilical vein endothelial cells as well as the role of IL-1α and IL-1ra in the angiogenesis process.</p><p><b>METHODS</b>Western blot was used to detect the expression of IL-1α and IL-1R1 protein in the colon cancer cell lines with different liver metastatic potential. We also examined how IL-1α and IL-1ra influence the proliferation and migration of umbilical vascular endothelial cells assessed by PreMix WST-1 assay and migration assay, respectively. Double layer culture technique was used to detect the effect of IL-1α on the proliferation and migration of vascular endothelial cells and the effect of IL-1ra on the vascular endothelial cells.</p><p><b>RESULTS</b>Western blot analysis showed that IL-1α protein was only detected in highly metastatic colon cancer HT-29 and WiDr cells, but not in the lowly metastatic CaCo-2 and CoLo320 cells.Migration assay showed that there were significant differences in the number of penetrated cells between the control (17.9±3.6) and 1 ng/ml rIL-1α group (23.2±4.2), 10 ng/ml rIL-1α group (31.7±4.5), and 100 ng/ml rIL-1α group (38.6±4.9), showing that it was positively correlated with the increasing concentration of rIL-1α (P<0.01 for all). The proliferation assay showed that the absorbance values were 1.37±0.18 in the control group, and 1.79±0.14 in the 1 ng/ml rIL-1α group, 2.14±0.17 in the 10 ng/ml rIL-1α group, and 2.21±0.23 in the 100 ng/ml rIL-1α group, showing a positive correlation with the increasing concentration of rIL-1α(P<0.01 for all). IL-1ra significantly inhibited the proliferation and migration of vascular endothelial cells (P<0.01). The levels of VEGF protein were (1.697±0.072) ng/ml, (3.507±0.064)ng/ml and (4.139±0.039)ng/ml in the control, HUVECs+ IL-1α and HUVECs+ HT-29 co-culture system groups, respectively, showing a significant difference between the control and HUVECs+ 10 pg/ml rIL-1α groups and between the control and HUVECs+ HT-29 groups (P<0.01 for both).</p><p><b>CONCLUSIONS</b>Our findings indicate that colon cancer cell-derived IL-1α plays an important role in the liver metastasis of colon cancer through increased VEGF level of the colon cancer cells and enhanced vascular endothelial cells proliferation, migration and angiogenesis, while IL-1ra can suppress the effect of IL-1α and inhibit the angiogenesis in colon cancer.</p>