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Journal of Medical Postgraduates ; (12)2003.
Article in Chinese | WPRIM | ID: wpr-584268

ABSTRACT

Objectine:To study the cytotoxicity of extracts of dominant indoor airborne fungal culture. Methods:Chinese hamster lung(CHL) cell culture was carried out in vitro.Cells were exposed to extracts of dominant indoor airborne fungal culture,then MTT method and CHL colonies assay were performed to detect their cytotoxicity.The release of cytosolic enzyme Lactate Dehydrogenase(LDH) and the fluidity of K~+,Ca~(2+) were analysed. Results:With the increase of the dose,the LDH levels in culture medium increased and the Ca~(2+) concentration inside cells increased while the K~+ concentration inside cells decreased accordingly. Conclusion:The extracts of dominant indoor airborne fungal culture could damage cell membrane,and have cytotoxicity.

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