ABSTRACT
Objective To investigate the chemical composition from the flowers of Callerya speciosa,and reveal the metabolites difference at different flowering periods based on metabolomics technology.Methods The primary and secondary metabolites,volatile chemical components in flowers of C.speciosa were analyzed combined by GC-MS and UPLC-Q-Exactive MS.Principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),and hierarchical cluster analysis(HCA)were performed to identify differential metabolites.Results A total of 332 compounds were identified by UPLC-Q-Exactive MS,mainly including secondary metabolites such as flavonoids,triterpenoids,phenylpropanoids.A total of 297 compounds were identified by GC-MS,mainly including primary metabolites and volatile chemical components,such as organic acids,amino acids,saccharides,heterocycles,alcohols.The PCA analysis demonstrated that the metabolites of the four flowering periods were divided into two groups:bud,initial bloom and blooming periods clustered into one group,while wilting period clustered into the other group,the main differences were filtered and identified as flavonoids and triterpenoids,organic acids,respectively.Compared to the upright type,the flowers of vine type contained more characteristic flavonoids as differential metabolites during the bud,initial bloom and blooming periods,and some flavonoids decrease gradually with the development of flowering.Conclusion The results indicated that the flowers of C.speciosa possessed abundant active flavonoid metabolites for further utilization,and the best harvest stage is initial bloom,the best harvest plant is vine type.This study provides a scientific basis for the scientific development and rational use of the flowers of C.speciosa.
ABSTRACT
Objective To compare the efficacy of laparoscopic surgery with combined medial and caudal approach and cephalic medial-to-lateral approach for right hemicolon cancer patients combined with incomplete ileus. Methods The clinical data of 98 right hemicolon cancer (T1- 4 M0) patients combined with incomplete ileus in Fukuang General Hospital of Liaoning Health Industry Group from January 2014 to December 2018 were retrospectively analyzed. All patients underwent laparoscopic right hemicolectomy, complete mesocolic excision (CME) and D3 lymphadenectomy. Among them, the combined medial and caudal approach was used in 50 patients (observation group), and the cephalic medial-to-lateral approach was used in 48 patients (control group). The operation time, bleeding volume, condition of lymph nodes dissected, length of hospital stay after operation and complication after operation were compared between 2 groups. Results Both groups successfully completed the operation, with no conversion to laparotomy and perioperative death. The bleeding volume and operation time in observation group were significantly lower than those in control group: (105.3 ± 22.6) ml vs. (309.6 ± 28.0) ml and (165.2 ± 17.9) min vs. (219.5 ± 21.5) min, and there were statistical differences (P﹤0.01 or ﹤0.05). There were no statistical differences in the number of lymph nodes dissected, proportion of lymph nodes dissected ≥ 12, number of positive lymph, length of hospital stay after operation and complication after operation between 2 groups (P＞0.05). Conclusions Laparoscopic surgery for right hemicolon cancer patients combined with incomplete ileus is safe and feasible. Compared with the cephalic medial-to-lateral approach, the combined medial and caudal approach can reduce the amount of intraoperative bleeding and shorten the operation time significantly.
ABSTRACT
Objective@#To compare the efficacy of laparoscopic surgery with combined medial and caudal approach and cephalic medial-to-lateral approach for right hemicolon cancer patients combined with incomplete ileus.@*Methods@#The clinical data of 98 right hemicolon cancer (T1-4 M0) patients combined with incomplete ileus in Fukuang General Hospital of Liaoning Health Industry Group from January 2014 to December 2018 were retrospectively analyzed. All patients underwent laparoscopic right hemicolectomy, complete mesocolic excision (CME) and D3 lymphadenectomy. Among them, the combined medial and caudal approach was used in 50 patients (observation group), and the cephalic medial-to-lateral approach was used in 48 patients (control group). The operation time, bleeding volume, condition of lymph nodes dissected, length of hospital stay after operation and complication after operation were compared between 2 groups.@*Results@#Both groups successfully completed the operation, with no conversion to laparotomy and perioperative death. The bleeding volume and operation time in observation group were significantly lower than those in control group: (105.3 ± 22.6) ml vs. (309.6 ± 28.0) ml and (165.2 ± 17.9) min vs. (219.5 ± 21.5) min, and there were statistical differences (P<0.01 or <0.05). There were no statistical differences in the number of lymph nodes dissected, proportion of lymph nodes dissected ≥ 12, number of positive lymph, length of hospital stay after operation and complication after operation between 2 groups (P>0.05).@*Conclusions@#Laparoscopic surgery for right hemicolon cancer patients combined with incomplete ileus is safe and feasible. Compared with the cephalic medial-to-lateral approach, the combined medial and caudal approach can reduce the amount of intraoperative bleeding and shorten the operation time significantly.