ABSTRACT
Objective To study the anticoagulant effects of Pheretima extracts and analyze the effective constituents. Methods Antithrombotic effects of Pheretima extracts were observed by determining the coagulation time, bleeding time of mice and the recalcification time of rabbit plasma in vitro, meanwhile the content of free amino acid in the extracts were determined. Results Pheretima extracts could prolong the coagulation time, bleeding time of mice, and the recalcification time of rabbit plasma in vitro. The content of free amino acid was rich in Pheretima extracts. Conclusion Free amino acid in Pheretima extracts may be the main anticoagulant effective constituents.
ABSTRACT
Objective To study the effect of oral Cefradine on pharmacokinetics of baicalin in rats in vivo. Methods The rats were divided into two groups: one was supplied with baicalin individually and the other was supplied with the combination of Cefradine and baicalin. Plasma concentrations of two groups were detected by HPLC with electrochemical detection (ECD). The pharmacokinetic parameters were calculated by statistical analysis and compared. Results The pharmacokinetic parameters of combination administration group were C_ max (782.63?469.37) ng/mL, AUC_ 0-24 h (8 407.86?3 476.14) ng/mL?h; while the individual administration group of baicalin was C_ max (2 645.62?601.42) ng/mL, AUC_ 0-24 h (28 952.90 ?5 731.42) ng/mL?h. Conclusion The main pharmacokinetic parameters show significant difference between the two groups (P