ABSTRACT
Objective To investigate the effect of heat treatment on the proliferation of, melanin synthesis and tyrosinase activity in cultured normal human melanocytes. Methods Normal human foreskin tissue was obtained by sterile circumcision and melanocytes were harvested by using methods for epidermal cell culture. Basic fibroblast growth factor (bFGF) was utilized as the primary mitogen to establish the culture system of normal human epidermal melanocytes. Masson-Fontana staining was proformed to identify melanocytes.Third-passage melanocytes were treated with hyperthermia at various temperatures (39 ℃, 41 ℃, 42 ℃, 43 ℃ and 45℃) for 1 hour a day for consecutive 3 days followed by the measurement of cell viability with MTT assay. The hyperthermia at optimized temperature was used to treat fourth-passage melanocytes for 1 hour a day for consecutive 3 days; subsequently, the tyrosinase activity were detected with L-Dopa as the substrate, and melanin content was determined in heat-treated and untreated (control) melanocytes. Results The hyperthermia at 42 ℃ exhibited the strongest promotive effect on the proliferation of melanocytes among these 5 hyperthermia conditions. After treatment with hyperthermia at 42 ℃ for 1 hour a day for consecutive 3 days, melanocytes showed an increment in tyrosinase activity by 36.4% and melanin synthesis by 78% compared with the untreated melanocytes (both P＜0.05). Conclusions Heat treatment can enhance the proliferation of cultured human melanocytes, promote their melanin synthesis and tyrosinase activity.
ABSTRACT
Objective To investigate the prevalence of dermatoses affecting officers and soldiers garrisoned southeast China,and to provide enough scientific basis for prevention and treatment of those dermatoses.Methods Epidemiological investigations were carried out on 324 officers and soldiers in both Zhejiang and Fujian military Regions,respectively.In order to get the dermatoses diagnosed correctly and the number of infected men/times calculated accurately,a large scale of clinical examination was performed by 3 senior dermatologists from the authors' hospital.Some medication and management were applied in therapy for those skin disorders.The elementary medical personnel in the troops were trained with a short time-training course to raise their vocational level.Results Tinea manuum and tinea pedis,acne,pigmented nevus,tinea corporis and tinea cruris,seborrheic dermatitis,eczema,pityriasis versicolor and lichen simplex chronicus were the eight common dermatoses affecting officers and soldiers garrisoned southeast China.The prevalence of tinea manuum and tinea pedis was highest one in all the 8 common dermatoses.158 out of 324 soldiers(48.8%)suffered from skin disorder.The prevalence of acne,pigmented nevus,tinea corporis and tinea cruris,seborrheic dermatitis,eczema,pityriasis versicolor and lichen simplex chronicus were 45.4%,19.3%,10.8%,12.3%,10.2%,7.4% and 7.1%,respectively.Conclusions Skin disorder is one of the most common diseases affecting officers and soldiers.There are different occurrences in different garrisoned region.The prevelence of tinea manuum and tinea pedis in Fujian military region is higher than that in Zhejiang military region,and the prevelence of tinea corporis and tinea cruris,eczema in Fujian military region is lower than that in Zhejiang military region.No significant difference was found in the prevalence of the eight common dermatoses between the two parts of soldiers originated separately from north or south regions of China.