ABSTRACT
Objective To study the effect of optimized atropine administration regimen on myopia in guinea pigs. Methods Forty six 21-day old guinea pigs were used for this study. Six were randomly selected as blank control, and the remaining 40 were randomly divided into 5 intervention groups: 1% atropine group, 0.01% atropine group, optimized group 1, optimized group 2, and saline group. One eye of the guinea pig in the intervention groups was randomly selected as the model eye and given form deprivation, and the contralateral eye was the self-control. The duration of intervention was 4 weeks. The diopter and axial length of guinea pig eyes were measured before the experiment and at each weekend. Choroid and sclera were measured after the experiment. Results The diopter of the model eyes in the 0.01% atropine group decreased rapidly. There was a significant difference before and after the experiment [(2.82±1.35)D vs (−0.64±0.20)D, P<0.01]. The diopter of model eyes decreased in 1% atropine group and optimized group 1, and the difference was statistically significant [(3. 50±1.14)D vs (1.38±1.15)D, P<0.05; (3.55±1.85)D vs (0.95±1.90)D, P<0.01]. In optimized group 2, the diopter of model eyes decreased, and there was no significant difference before and after the experiment [(1.36±1.61)D vs (2.93±1.42)D, P>0.05). After form deprivation, the axial length in 1% atropine group did not change significantly (P>0.05). The axial length in other intervention groups was extended to varying degrees. The thickness of choroid and sclera in 1% atropine group, optimized group 1 and optimized group 2 were greater than that in 0.01% atropine group. Conclusion The two optimized dosing regimens worked better than 0.01% atropine in inhibiting myopia in guinea pigs with form deprivation, and were similar to 1% atropine.
ABSTRACT
Glaucocalyxin A (GLA) is a kind of diterpenoid enantiomers with organic chemical structure of en-15-oxo-16-kaurene.It has various pharmacological activities,such as cardiovascular and endothelium cellular protection,anti-blood coagu-lation,anti-Hepatitis B Virus (HBV),anti-tumor,anti-bacteria,anti-inflammation,hypoxic tolerance,immunomodulation and Ca2+ concentration regulation effect,where as its in vivo safety is quite good.As a folk medicine,it is conventionally used to treat hepatitis,gastritis,mastitis,stomachache and arthralgia.It is also used for ischemic and/or hypoxic cardio-cerebrovas-cular diseases such as coronary heart diseases,stenocardia and chronic cerebral circulation insufficiency in clinic.This review gives a summary of the pharmacological effects,biological mechanism,and toxicity of Glaucocalyxin A.