A multicenter study of reference intervals for 15 laboratory parameters in Chinese children / 中华儿科杂志

Objective@#To establish comprehensive laboratory reference intervals for Chinese children.@*Methods@#This was a cross-sectional multicenter study. From June 2013 to December 2014, eligible healthy children aged from 6-month to 17-year were enrolled from 20 medical centers with informed consent. They were assessed by physical examination, questionnaire survey and abdominal ultrasound for eligibility. Fasting blood samples were collected and delivered to central laboratory. Measurements of 15 clinical laboratory parameters were performed, including estradiol (E2), testosterone(T), luteinizing hormone(LH), follicle-stimulating hormone(FSH), alanine transaminase(ALT), serum creatinine(Scr), cystatin C, immunoglobulin A(IgA), immunoglobulin G(IgG), immunoglobulin M(IgM), complement (C3, C4), alkaline phosphatase(ALP), uric acid(UA) and creatine kinase(CK). Reference intervals were established according to central 95% confidence intervals for reference population, stratified by age and sex.@*Results@#In total, 2 259 children were enrolled. Finally, 1 648 children were eligible for this study, including 830 boys and 818 girls, at a mean age of 7.4 years. Age- and sex- specific reference intervals have been established for the parameters. Reference intervals of sex hormones increased gradually with age. Concentrations of ALT, cystatin C, ALP and CK were higher in children under 2 years old. Serum levels of sex hormones, creatinine, immunoglobin, CK, ALP and urea increased rapidly in adolescence, with significant sex difference. In addition, reference intervals were variable depending on assay methods. Concentrations of ALT detected by reagents with pyridoxal 5'-phosphate(PLP) were higher than those detected by reagents without PLP. Compared with enzymatic method, Jaffe assay always got higher results of serum creatinine, especially in children younger than 9 years old.@*Conclusion@#This study established age- and sex- specific reference intervals, for 15 clinical laboratory parameters based on defined healthy children.

Research progress in fusion expression of antimicrobial peptides / 生物工程学报

Antimicrobial peptides (AMPs) are of great significance in the field of food, feed and medicine due to their wide spectrum of antimicrobial activity and new mechanism of action different from conventional antibiotics. AMPs production from natural sources is usually limited, and chemical synthesis is not economically practical, especially for the production of long peptides. Therefore, heterologous expression of AMPs has been widely studied as an alternative, and fusion expression plays an important role in increasing production. The present review mainly focuses on the types and bioactivities of AMPs. In addition, the recent strategies to the most commonly used carrier proteins for fusion expression of AMPs and prospects for future research were also discussed.

The expression of β_2 AR and IL-12 and their interaction with childhood asthma / 临床儿科杂志

Objective To study the relationship between asthma and mRNA expression of β_2AR and IL-12 gene;to determine the expression level of β_2AR, IL-12 related to CysLT_1-receptor;to analyze the correlation between the severity of asthma and the level of the genes expression. Methods White blood cells was drawn from peripheral blood mononuelear cells of asthmatic children. DNA was taken and related genes were analyzed for half quantitative analysis by D-congeal glue analyzer software. Results The level of IL-12. β_2AR mRNA expression in the asthma group is obviously lower than healthy controls (P < 0.01) ;when asthma attacks, the expression of IL-12 is positively correlated to that of β_2AR (r = 0.34, P = 0.001) and negatively correlated to that of CysLT_1-receptor (r = -0.92, P = 0.001), the expression of β_2AR is negatively correlated to the expression of CysLT_1-receptor (r = -0.85, P = 0.003). The express of IL-12 is not related to the severity of asthma (P = 0.16), while there was a negative correlation between the expression of β_2AR and the severity of asthma (P = 0.003). Conclusions The expression level of IL-12, β_22AR in the asthmatic children is obviously lower than normal;The expression level of IL-12 is positively correlated to β_2AR in PBMC of asthmatic children;The expression level of CysLT_1-receptor is negatively correlated to IL-12 and β_2AR in the PBMC of asthmatic children;The expression of β_2AR is negatively correlated to the severity of asthma.

Changes of serum IL-1、IL-6 and IL-8 in children with bronchial asthma / 临床儿科杂志

Serum IL-1,IL-6 and IL-8 were determined by subjects-using enzyme linked immunosorbent assay (ELISA technique) in 40 children with bronchial asthma consisting of 20 cases in the acute stage and 20 cases in the remission stage,and another 20 health children as normal control,respectively.The results showed that the levels of serum IL-1(11.9pg/ml±2.84pg/ml),IL-6(0.75ng/ml±0.3ng/ml)and IL-8(0.08ng/ml±0.05ng/ml)in the acute stage were significantly higher than those in the remission stage(8.41pg/ml±0.05pg/ml(IL-1),0.49ng/ml±0.08ng/ml(IL-6)and 0.02ng/ml±0.01ng/ml(IL-8)) and in the normal children (8.4pg/ml±0.32pg/ml(IL-1),0.4ng/ml±0.09ng/ml(IL-6)and 0.02ng/ml±0.02ng/ml(IL-8)),respectively.Further more,there were a significant positive correlation between IL-1 and IL-6 or IL-8(P＜0.01).It is concluded that IL-1 may involve in the pathologic process of asthma like IL-6 and IL-8,and an inflammatory reaction may still exist in the airway during the remission stage of asthma. The imbalances of cytokine network may probably be important reason as a molecular basis in the asthma pathogenesis.It is suggested that it can be valuable to predict an acute attack of asthma when the levels of these cytokines increase.

Intravenous immunoglobulin in adjuvantly treatment of severe pneumonia of infant / 中国新药与临床杂志

AIM: To study the clinical efficacy and safety of intravenous immunoglobulin (IVIG) for infant with severe pneumonia.　METHODS: Seventy patients of infant with severe pneumonia were divided into 2 groups. Patinets (n=50) of the trea- tment group (M 30, F 20; age 6 mo± s 5 mo) were treated with IVIG and conventional drugs, IVIG 400 mg*kg-1*d-1 for 3 d. Patinets (n=20) of the control group (M 12, F 8; age 7 mo±7 mo) were treated with conventional drugs.　RESULTS: The duration of fever, tachypnea, high heart rate shortened. The gasp and rales disappeared earlier. The cardiac failure recovered faster than the control group. There was significantly diffference statistically (P<0.05). The level of IgG, IgA raised after treatment. There was significantly difference statistically (P<0.01). The level of CD+3, CD+4, CD+8 after treatment also raised significantly compared with that before treatment (P<0.05). IL-6 and IL-8 after treatment reduced. There was significantly difference statistically (P<0.05).　CONCLUSION: IVIG is safe and effective drug in adjuvant treating severe pneumonia of infant, specially RSV pneumonia.