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Objective:To investigate the effects of repetitive transcranial magnetic stimulation (rTMS) on learning-memory and the expression of synaptic plasticity proteins in hippocampus of rats with post-stroke sleep deprivation.Methods:A total of 28 SPF grade healthy male Wistar rats with 8-week old were randomly divided into four groups (control group, sham operation group, model group and rTMS group) according to random number table method, with 7 rats in each group. The rats in the model group and the rTMS group were treated with middle cerebral artery occlusion and p-chlorophenylalanine intraperitoneal injection to establish the post-stroke sleep deprivation model. The rats in the rTMS group were treated with rTMS intervention for consecutive 14 days after modeling. The rats in the sham operation group were only separated arteries but not ligated and inserted. The rats in control group were fed normally. The open field test (OFT) was used to observe the autonomous behavior of rats.The water maze test(WMT) was used to observe the spatial learning and memory ability of rats.The content of tyrosine kinase receptor type B(TrkB) in hippocampus was detected by Western blot.The expressions of brain-derived neurotrophic factor(BDNF) and immediate early gene c-fos in hippocampus were detected by immunofluorescence.The morphology and structure of neurons in hippocampus were observed by optical microscopy and transmission electron microscopy. SPSS 21.0 software was used for statistical analysis, and repeated measurement ANOVA was used for the escape latency data, one-way ANOVA was used for the comparison of other data among multiple groups, and LSD test was used for further pairwise comparison.Results:(1) The OFT results showed that there were statistical differences in the numbers of crossing squares, upright times and total points of rats in the four groups after intervention ( F=27.638, 10.425, 30.690, all P<0.001). The numbers of crossing squares ((72.71±10.10)), upright times ((6.57±0.87)times) and total points ((79.29±10.03) points) of rats in rTMS group were all higher than those in model group after intervention ((43.71±6.96), (3.43±0.65)times, (47.14±6.82)points) (all P<0.05). As for the escape latency of WMT among the four groups of rats, the interaction effect was not significant( F=1.108, P=0.37), and the time main effect( Ftime=27.295, Ptime<0.01) and group main effect ( Fgroup=8.691, Pgroup<0.01) were significant after rTMS intervention.On the 3rd and 4th day, the escape latency of rTMS group rats was lower than that of the model group (both P<0.01). There were statistically significant differences in the numbers of crossing platform, swimming distance and residence time in target quadrant of rats in the four groups after intervention( F=8.569, 3.308, 3.547, all P<0.05). The numbers of crossing platform ((2.00±0.31)times), swimming distance in target quadrant ((196.95±24.57) cm) and residence time ((17.72±1.36)s) of rats in rTMS group were all higher than those in model group after intervention ((1.57±0.30)times, (146.61±4.79) cm, (13.58±0.98)s)(all P<0.05). (2)Optical microscopy and transmission electron microscopy showed that the hippocampal cells arranged irregularly, the organelles' integrity was destroyed in the model group compared with the normal control group. In rTMS group the arrangement and structure of nerve cells in the hippocampus were improved after rTMS intervention. (3) The immunofluorescence results showed that c-fos (1.49±0.09) and BDNF (0.84±0.06) in the hippocampus of rats in rTMS group were both higher than those in model group ((1.24±0.12), (0.48±0.08))(both P<0.05). The Western blot results showed that the expression level of TrkB (1.81±0.03) in the hippocampus of rats in rTMS group was higher than that in model group (0.96±0.02) ( P<0.05). Conclusion:The rTMS can improve the learning-memory ability and autonomous capacity of rats with post-stroke sleep deprivation, which may be related to promoting the expression of c-fos, BDNF and TrkB in hippocampus tissue.
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Objective To prepare a liquid composite quality control material of D-dimer,and then to inves-tigate its stability.Methods Pooled human serum without infection[negative for the antibodies to human im-munodeficiency virus(HIV),hepatitis C virus(HCV)and hepatitis B surface antigen(HBsAg),TP-IgG]was used as the medium for the composite quality control material preparation to investigate its precision and sta-bility.Results The within-run precision of our product was 1.83% which was < 1/4 CLIA'88 acceptable per-formance range.The between-run precision was 3.02% which was < 1/3 CLIA'88 acceptable performance range.The quality control material was stable for 12 months at -20 ℃.The stability[coefficient of variation (CV%)of each item of the quality control material was<1/2 CLIA'88 acceptable performance range.Conclu-sion T he liquid composite lipid quality control material has good homogeneity and stability,and it can be used for D-dimer testing the internal quality control.
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Objective T he effects of different intensity aerobic exercise on the coagulation function of stroke were investigated by detection coagulation index of stroke patients before and after different intensity of aerobic training.Methods 120 patients with neurological initial stroke were enrolled from January to Decem-ber in 2016.According to the cardiopulmonary exercise test,the patients were divided into medium-intensity aerobic exercise group,low-intensity aerobic exercise group and control group.The control group maintained the basic medical treatment of neurological primary stroke.The medium-intensity aerobic exercise group and low-intensity aerobic exercise group were on the basis of the control group with different intensity by stampe-ding power bike training.The plasma fibrinogen(FIB),D-Dimer(D-D),activated partial thromboplastin time (APTT),prothrombin time(PT),international normalized ratio(INR)and thrombin time(TT)were detected and analyzed before and after exercise training.Results The results showed there were no significant differ-ence in coagulation index between the three groups before exercise training(P>0.05).The levels of FIB and D-D in medium-intensity aerobic exercise group after the training were significantly lower than before the training(P<0.05).The levels of FIB in low-intensity aerobic exercise group after the training were signifi-cantly lower than before the training(P<0.05).There were no significant difference between in others coagu-lation index between two groups(P>0.05).There were no significant difference in coagulation index between before and after the basic treatment in control group(P>0.05).After exercise training,the levels of FIB in the moderate intensity group was significantly lower than the low intensity group(P<0.05),and then the low intensity group was significantly lower than control group(P<0.05).Conclusion The medium and low-intensity aerobic exercise could influence the coagulation function of stroke patients,particularly in the medi-um-intensity.It provided the scientific basis for individual aerobic exercise combined with drugs to prevent stroke recurrence.
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Objective To investigate the apply value of the Visual FoxPro database management system in statistic analysis of blood lipid .Methods The blood lipid detection data of 2397 cases undergoing physical examination in the Chongqing Tumor Insti-tute were selected .The Visual FoxPro 6 .0 database management system was adopted to conduct the statistical analysis on total cho-lesterol(TC) ,triglyceride(TG) ,low density lipoprotein cholesterol(LDL) and high density lipoprotein cholesterol(HDL) in various age groups .The blood lipid situation in various ages periods was analyzed .Results The overall dyslipidemia rate in the research subjects was 45 .44% ,in which 57 .62% for male and 33 .80% for female .Serum TC ,TG ,HDL-C and LDL-C mean levels were (4 .94 ± 0 .96) ,(1 .05 ± 2 .35) ,(2 .83 ± 0 .79)and(1 .17 ± 0 .27)mmol/L in male cases ,and(4 .86 ± 0 .91) ,(0 .83 ± 1 .57) ,(2 .64 ± 0 .80) and (1 .35 ± 0 .29)mmol/L in female cases respectively .Conclusion The Visual FoxPro 6 .0 database management system can be very convenient to screen and statistically analyze the blood lipid levels in different age groups and has the advantages of high efficiency ,rapidness and simpleness .
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Objective To explore the clinical value of laparoscopy combined with radionuclide imaging in the diagnosis and treatment of pediatric gastrointestinal hemorrhage, especially for Meckel’s diverticulum and double intestine. Methods ~ 99m Tc~-pertechnetate abdominal scintigraphy was performed in 22 children with a history of recurrent hemafecia or melena in this hospital from December 1998 to December 2005. All the children were given a laparoscopic exploration. Results Among the 22 patients, scintigraphy showed positive findings in 18 patients and negative, 4 patients. The positive patients were all confirmatively diagnosed by laparoscopic surgery and pathological examinations, including Meckel’s diverticulum in 14 patients and duplications of alimentary tract in 4 patients. In the other 4 patients with negative results, no organic pathologic changes was identified by laparoscopy in 3 patients and Meckel’s diverticulum was found after laparoscopic exploration in 1 patient. Conclusions Radionuclide imaging is an important method in the diagnosis of gastrointestinal tract hemorrhage in children and provides scientific basis for surgical intervention. The combination of laparoscopy and radionuclide imaging not only has values in the diagnosis and treatment of pediatric gastrointestinal hemorrhage, but also minimizes the blindness and trauma of exploratory laparotomy.
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Objective To study a new operative procedure for hydrocele in children under mini-lapascope.Methods 103 children with hydrocele aged from 1 to 9 years old were performed by suture around internal rings under mini-laparoscope from November 2000 to March 2002. Results The operative time was (5～8) minutes and hospital stay (4～5) days. The incision didn't need suture and there was no obvious scar after operation. All cases were followed up at 1st month, 6th month and 1st year postoperatively. All cases recovered except for 2 cases recurred at 1st month postoperatively. Conclusions Suture around internal rings under mini-laparoscope is a minimally invasive and simple method for the treatment of hydrocele in children. It can find and deal with hidden patent internal rings.
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Objective To explore the clinical effects of laparoscopic hernioplasty in patient over 70 years old. Methods We utilized the modified laparoscopic intraperitoneal onlay mesh (IPOM) to treat the inguinal hernia in 69 elderly patients. Results The laparoscopic operations were completed in all the 69 patients. The operative time was 20～30 min (mean, 25 min). No operative complications took place and the intraoperative blood loss was hardly seen. The duration of hospitalization was 5～7 days. The patients recovered smoothly, without wound infection or scrotum hematoma. Follow-up observations for 6～24 months in the 69 patients found no recurrence. Conclusions Modified laparoscopic IPOM treating inguinal hernia in elderly patients is feasible, on the premise of proper perioperative management.