ABSTRACT
Objective To identify a myeloid differentiation factor 88(MyD88)in Oncomelania hupensis,and characterize the role of MyD88 against Schistosoma japonicum infection. Methods The complete cDNA of MyD88 in O. hupensis was ob-tained by using rapid amplification of cDNA ends(RACE),and homologues sequences and conserved domains were aligned and the structure of MyD88 was predicted either. A phylogenetic tree of MyD88 was further constructed with other species. In ad-dition,the mRNA expression level of O. hupensis MyD88 before and after S. japonicum infection was investigated by real-time quantitative PCR(RT-qPCR). Results The cDNA of O. hupensis MyD88 consisted of 1406 bp open reading frame(ORF),en-coding 468 amino acid residues,which contained death domain and Toll/interlrukin-1 receptor(TIR)domain,the typical fea-tures of MyD88 family proteins. The predicted amino acid sequence of O. hupensis MyD88 shared 38%-52%identity with other mollusc. O. hupensis MyD88 was phylogenetically closeted to Biomphalaria glabrata MyD88. The O. hupensis MyD88 existed in all selected tissues and expressed highly in hemocyte,up-regulated after S. japonicum infection in all selected tissues except cephalopodium,especially higher in whole snail and hemocyte. Conclusion MyD88-dependent signaling pathway is present in O. hupensis and plays an important role in innate immune response against S. japonicum infection.
ABSTRACT
Objective To understand the immigrants' recognition of schistosomiasis and their relevant behaviors in the South-North Water-Diversion Middle-line Project areas in Hubei Province.Methods The investigation objects were selected by the method of stratified sampling among immigrants in the project areas and were surveyed through oral interview and questionnaire.Results A total of 1 010 immigrants were investigated and 1 005 questionnaires were indentified as effective ones.The awareness rates of schistosomiasis and the correct rates of related behaviors among the immigrants were still not satisfying.Conclusions The immigrants' recognition of schistosomiasis and their relevant behaviors in South-North Water-Diversion Middle-line Project areas in Hubei Province still need to be improved through health education and other measures.
ABSTRACT
Proteomics is an important high throughout method in modern life science.In this paper,the definition,background and methods used in proteomics were introduced,and the last part was focused on its application in parasitology.