ABSTRACT
The mesencephalic astrocyte-derived neurotrophic factor (MANF) has been recently identified as a neurotrophic factor, but its role in hepatic fibrosis is unknown. Here, we found that MANF was upregulated in the fibrotic liver tissues of the patients with chronic liver diseases and of mice treated with CCl4. MANF deficiency in either hepatocytes or hepatic mono-macrophages, particularly in hepatic mono-macrophages, clearly exacerbated hepatic fibrosis. Myeloid-specific MANF knockout increased the population of hepatic Ly6Chigh macrophages and promoted HSCs activation. Furthermore, MANF-sufficient macrophages (from WT mice) transfusion ameliorated CCl4-induced hepatic fibrosis in myeloid cells-specific MANF knockout (MKO) mice. Mechanistically, MANF interacted with S100A8 to competitively block S100A8/A9 heterodimer formation and inhibited S100A8/A9-mediated TLR4-NF-κB signal activation. Pharmacologically, systemic administration of recombinant human MANF significantly alleviated CCl4-induced hepatic fibrosis in both WT and hepatocytes-specific MANF knockout (HKO) mice. This study reveals a mechanism by which MANF targets S100A8/A9-TLR4 as a "brake" on the upstream of NF-κB pathway, which exerts an impact on macrophage differentiation and shed light on hepatic fibrosis treatment.
ABSTRACT
OBJECTIVE@#To investigate the effects of human amniotic mesenchymal stem cell（AMSC） on acute graft-versus-host disease (aGVHD) in xenotransplatation.@*METHODS@#NPG mice were injected with human PBMNC via tail vein to establish a xenografted aGVHD model. The mice in the experimental group were divided into PBMNC infusion group and PBMNC+AMSC co-infusion group, the general condition, survival time and manifestations of aGVHD were observed, the body weight and blood routine indicators were detected, the pathological changes of aGVHD target organs (lung, liver, spleen, small intestine) were observed by HE staining, and the levels of human T cells in peripheral blood, tissues and organs of mice was detected by flow cytometry.@*RESULTS@#The manifestations of aGVHD (lassitude hunchback, shrub, weight reduction, etc.) and the pathological damage of the target organs (lung, liver, spleen, intestine) in PBMNC+AMSC co-infusion group were lighter than those in PBMNC infusion group. Moreover, the PBMNC and AMSC co-infusion significantly reduced the implantion proportion of human T lymphocytes (CD3, CD45) in mice and increased the ratio of CD4/CD8.@*CONCLUSION@#Infusion of human-derived AMSC can attenuate the manifestations of aGVHD in mouse xenografts to a certain level, and improve the pathological damage of receptor target organs.
ABSTRACT
Objective To investigate the effects of motivational interview on compliance,negative emo-tions and quality of life in elderly patients with biliary acute pancreatitis. Methods 56 patients with senile biliary acute pancreatitis who underwent routine interventions from September 2015 to August 2016 were selected as the control group. Patients in the interventional intervention interviews between September 2016 and August 2017 56 cases of elderly patients with gallstone acute pancreatitis were selected as the observation group. Comparisons of treatment adherence,negative emotions and quality of life before and after intervention for one month were com-pared between the two groups. Results After 1 months'intervention,the treatment compliance of the observation group was better than that of the control group. The difference was statistically significant(P < 0.05). After 1 months' intervention,the negative emotion scores of the observation group were all lower than those of the control group,and the scores of all quality of life were significantly higher than those of the control group,the difference was statistically significant(P < 0.05). Conclusions The motivational interview is effective in elderly patients with biliary acute pancreatitis,which can effectively improve the treatment compliance,reduce the negative emo-tions and improve the quality of life,which is worth promoting.
ABSTRACT
<p><b>OBJECTIVE</b>To observe therapeutic effect of aponeurotic system penetration needling on peripheral facial paralysis.</p><p><b>METHODS</b>One hundred and ten cases of peripheral facial paralysis were randomly divided into a Jingjin group (n=68) and a control group (n=42). The therapeutic effects of acute stage, resting stage and sequela stage, and the relation between the facial nerve lesion degree and the therapeutic effect were investigated.</p><p><b>RESULTS</b>The effective rates of the two needling methods were respectively 98.5% and 90.5%, the Jing1in group being better than the control group (P < 0.05); at the acute stage, the therapeutic effect of acupuncture was obvious, and the therapeutic effect at the sequela stage and for the patient of nerve faulty type in the Jingjin group were better than that of the control group (P < 0.05).</p><p><b>CONCLUSION</b>Acupuncture and moxibustion has definite therapeutic effect on facial paralysis at the acute stage and in the patient of nerve active type, and aponeurotic system penetration needling can be used for the patient of facial paralysis at the sequela stage or with nerve faulty type.</p>