ABSTRACT
Objective To evaluate the rapid diagnosis of bacterial and (or) fungal endophthalmitis by multiplex polymerase chain reaction (MPCR). Methods MPCR was performed to detect the DNA segment of bacteria and (or) fungi from standard strains and 41 samples of intraocular fluid or vitreous from 38 patients (3 with double eyes and 35 with single), and the results were compared with the cultured bacteria and fungi. Results Five hours after detected by MPCR, bacteria and (or) fungi in 34 out of 41 samples (82.9%) from patients were detected,including bacteria in 26,fungi in 6,and both bacteria and fungi in 2. The positive rate of MPCR was obviously higher than the cultured ones( ? 2 =9.60, P
ABSTRACT
The platelet aggregation and release reaction, the haemostatic factors and fibrinolytic activity were measured in 36 patients with early retinal vein occlusion (RVO) and 20 control subjects. The mean levels of platelet aggregation, plasma fibronection, factor Ⅷ antigen and plasminogen were significantly higher in the patients than in the controls. These findings showed that the blood hypercoagulant state was present in patients with RVO, and these abnormalities in blood coagulation could play an important role in the onset and development of RVO and its ischaemic complications.