Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add filters








Language
Year range
1.
Chinese Journal of Applied Physiology ; (6): 74-79, 2009.
Article in Chinese | WPRIM | ID: wpr-252705

ABSTRACT

<p><b>AIM</b>The mechanisms of cytokines in regulating oocyte maturation is still little known. The present study attempt to investigate whether the protooncogene of c-erbB2, c-myb are involved in introducing of cytokines to regulate oocyte maturation.</p><p><b>METHODS</b>This research used mouse GV stage oocyte culture model in vitro and RT-PCR, Western blotting method to explore the effect of EGF, TNFalpha, ET-1 and NO on oocyte maturation; to analyze the c-erbB2 mRNA and c-myb mRNA expression and the phosphorylation of MAPK and cyclinB1 expression in oocytes affected by above cytokines.</p><p><b>RESULTS</b>EGF(10 microg/L) stimulated meiosis of oocytes significantly, the level of c-erbB2 mRNA, c-myb mRNA were increased, and promoted the phosphorylation of MAPK and cyclinB1 expression; TNFalpha (1 microg/L) and ET-1 ((10(-1) mol/L) had the results to EGF. Low dose of SNP (10(-5)mol/L) had no effect on oocyte maturation, but could significantly reverse the suppression of dbcAMP on oocyte maturation.</p><p><b>CONCLUSION</b>c-erbB2 and c-myb were involved in introducing of cytokines to regulate oocyte maturation, might be the middle link in connection of the cytokines with MAPK and MPF in regulation oocyte maturation.</p>


Subject(s)
Animals , Female , Mice , Cells, Cultured , Cytokines , Physiology , Epidermal Growth Factor , Physiology , Intercellular Signaling Peptides and Proteins , Physiology , Maturation-Promoting Factor , Genetics , Metabolism , Mitogen-Activated Protein Kinases , Metabolism , Oocytes , Cell Biology , Physiology , Oogenesis , Physiology , Proto-Oncogene Proteins c-myb , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , Receptor, ErbB-2 , Genetics , Metabolism , Tumor Necrosis Factor-alpha , Physiology
SELECTION OF CITATIONS
SEARCH DETAIL